Reduction of Salmonella Typhimurium Cecal Colonisation and Improvement of Intestinal Health in Broilers Supplemented with Fermented Defatted ‘Alperujo’, an Olive Oil By-Product

Abstract

Salmonella spp. contaminates egg and poultry meat leading to foodborne infections in humans. The emergence of antimicrobial-resistant strains has limited the use of antimicrobials. We aimed to determine the effects of the food supplement, fermented defatted ‘alperujo’ (FDA), a modified olive oil by-product, on Salmonella Typhimurium colonisation in broilers. One hundred and twenty 1-day-old broilers were divided into four experimental groups—two control groups and two treated groups, and challenged with S. Typhimurium at day 7 or 21. On days 7, 14, 21, 28, 35, and 42 of life, duodenum and cecum tissue samples were collected for histopathological and histomorphometric studies. Additionally, cecum content was collected for Salmonella spp. detection by culture and qPCR, and for metagenomic analysis. Our results showed a significant reduction of Salmonella spp. in the cecum of 42-day-old broilers, suggesting that fermented defatted ‘alperujo’ limits Salmonella Typhimurium colonization in that cecum and may contribute to diminishing the risk of carcass contamination at the time of slaughter. The improvement of the mucosal integrity, observed histologically and morphometrically, may contribute to enhancing intestinal health and to limiting Salmonella spp. colonisation in the host, mitigating production losses. These results could provide evidence that FDA would contribute to prophylactic and therapeutic measures to reduce salmonellosis prevalence in poultry farms.

Lactic Acid Bacteria from Gamecock and Goat Originating from Phitsanulok, Thailand: Isolation, Identification, Technological Properties and Probiotic Potential

“…The majority of human diseases are linked to the consumption of eggs and chicken meat, which are typically contaminated during the slaughtering process and spread throughout the food chain. Salmonellosis in chicken also causes significant costs in the poultry industry in terms of mortality, poor growth, and egg production reduction [ 55 - 57 ].…”

Effects of Hexane Extract of Garlic on Hematological, Biochemical and Histological Parameters in F1 Crossbred Chicks Non-Infected and Infected with Salmonella typhimurium

“…For this reason, it is essential to control salmonellosis by increasing the natural resistance of chickens, thereby reducing the socioeconomic damages worldwide in the one hand and fight against antibiotic resistance on the other hand. Several alternatives, such as probiotics, prebiotics, phytobiotics and feed additive have been used worldwide to combat the spread of salmonella infection (Rebollada-Merino et al 2020;Sugiharto et al 2020).…”