Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies

Tojo, Shinichiro J.; Yokota, Shinichi; Koike, Haruhiko; Schultz, Jonathan; Hamazume, Yasuki; Emi, Mitsuru; Yamada, Kazuto; Hayashi, Mikio; Paulson, James C.; Morooka, Shigenori

doi:10.1093/glycob/6.4.463

Cited by 49 publications

(29 citation statements)

References 35 publications

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“…A single 10 mg/kg intravenous dose in these experiments significantly reduced myocardial necrosis as measured directly, or as reflected by postmyocardial ischemia/ reperfusion plasma creatine kinase measurements and preserved cardiac contractility and endothelial cell function. Similar results with sialyl Lewis x type oligosaccharides have been reported in dog (28) and rat (29) myocardial reperfusion injury models, where, like in the cat, P-and L-selectin-dependent neutrophil recruitment contributes substantially to injury. Reductions in tissue injury have also been achieved with this glycan in a rat model of E-selectin-dependent cerebral ischemia and reperfusion injury (30).…”

supporting

confidence: 86%

Therapeutic inhibition of carbohydrate-protein interactions in vivo.

Lowe

Ward²

1997

J. Clin. Invest.

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supporting

confidence: 86%

Therapeutic inhibition of carbohydrate-protein interactions in vivo.

Lowe

Ward²

1997

J. Clin. Invest.

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“…We chose 15 and 10 minutes of occlusion because in rats there is extensive infarction beyond 15 minutes 36 and we deemed Ͻ10 minutes of ischemia as not representative of ACS. An inherent limitation of our model was that the goal of simulating ischemia without infarction was not consistently achieved; all but 6 rats had some necrosis by triphenyl tetrazolium chloride staining, albeit relatively limited.…”

Section: Study Limitationsmentioning

confidence: 99%

Myocardial Ischemic Memory Imaging With Molecular Echocardiography

et al. 2007

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Background-Diagnosing acute coronary syndrome in patients presenting with chest discomfort is a challenge. Because acute myocardial ischemia/reperfusion is associated with endothelial upregulation of leukocyte adhesion molecules, which persist even after ischemia has resolved, we hypothesized that microbubbles designed to adhere to endothelial selectins would permit echocardiographic identification of recently ischemic myocardium. Methods and Results-Lipid microbubbles (diameter, 3.3Ϯ1.7 m) were synthesized. The selectin ligand sialyl Lewis

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“…25 To evaluate the involvement of P-selectin in leukocyte-or platelet-endothelial interactions, we used SHR and WKY 12 hours after LPS injection. ARP2-4 (2 mg/kg) was administered intravenously in each strain 5 minutes before the injection of AO solution or fluorescently labeled platelets (nϭ6 per each experiment).…”

Section: P-selectin Involvement In the Interactionsmentioning

confidence: 99%

Interactions Between Blood Cells and Retinal Endothelium in Endotoxic Sepsis

et al. 2000

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Abstract-Platelets and leukocytes are thought to play a leading role in the pathogenesis of many inflammatory conditions.To recruit flowing blood cells to the inflammatory region, it would be necessary for them to interact with vascular endothelial cells. Recently, many reports have indicated the resistance of spontaneous hypertensive rats (SHR) to endotoxic sepsis. Their resistance might be derived from suppressed interaction between these blood cells and endothelial cells. Therefore, SHR and age-matched Wistar-Kyoto rats (WKY) were induced with endotoxic sepsis by intravenous injection of lipopolysaccharide (LPS). At 4, 12, 24, and 48 hours after induction, leukocyte-endothelial interactions in the retina were evaluated in vivo with acridine orange digital fluorography. Fluorescently labeled platelets were also injected to investigate platelet-endothelial interactions in the retina in endotoxic sepsis. Leukocyte rolling in SHR after LPS injection was significantly suppressed; the maximum number of rolling leukocytes was reduced by 80.1% at 12 hours after LPS injection in SHR compared with WKY. Subsequent leukocyte infiltration into the vitreous cavity was significantly inhibited in SHR. Furthermore, platelet-endothelial interactions in the retina were also suppressed in SHR treated with LPS. The maximum numbers of rolling and adherent platelets were reduced by 59.5% and 62.6%, respectively, in SHR compared with WKY. In both strains, leukocyte-and platelet-endothelial interactions were substantially inhibited by the blocking of P-selectin. These suppressed interactions could contribute to the reduction of leukocyte-and platelet-mediated tissue injury in endotoxic sepsis in SHR, resulting in their resistance to endotoxemia. (Hypertension. 2000;36:250-258.)

show abstract

Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies

Cited by 49 publications

References 35 publications

Therapeutic inhibition of carbohydrate-protein interactions in vivo.

Therapeutic inhibition of carbohydrate-protein interactions in vivo.

Myocardial Ischemic Memory Imaging With Molecular Echocardiography

Interactions Between Blood Cells and Retinal Endothelium in Endotoxic Sepsis

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