Reduction of INTERLEUKIN-1.BETA. Expression in Rheumatoid Arthritis by Linear Polarized Near Infrared Light Irradiation

Abstract

Background: Rheumatoid arthritis (RA) is a systemic autoimmune disorder that involves inflammation and pain of joints. Low-level light therapy (LLLT) is being evaluated for treating RA. However, the molecular basis mechanism underlying the effectiveness of linear polarized near infrared light irradiation (LPIL) is unclear. It has been reported that interleukin 1ß (IL-1ß) plays a key role in the progression of RA. Aim: The objective of this study was to determine whether LPIL (Super Lizer TM ; SL) decrease gene expression and production IL-1ß in human RA synovial cells, MH7A, and RA joint tissue of collagen-induced-rheumatoid arthritis (CIA) rats. Materials and Methods: IL-1ß challenged MH7A and CIA rat joints were irradiated with SL. Total RNA was isolated from MH7A, and gene expression profiles were analyzed using DNA microarray. The mRNA levels were confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. The production of IL-1ß in rat knee joints was analyzed by immunohistochemistry. Results: DNA microarray analysis showed that IL-1ß increased IL-1ß gene expression in MH7A, and SL significantly reduced its IL-1ß mRNA level. The reduction of IL-1ß mRNA level by SL was successfully confirmed by reverse RT-PCR and real-time PCR. SL reduced the swelling of CIA rat knee joints, and the immunohistochemical study demonstrated that a strong IL-1ß staining in synovial membrane tissue of CIA rat joint, and the immuno-staining was significantly reduced by SL. Conclusion: Since autocrine IL-1ß production has been identified to be an important proinflammatory cytokine in the pathogenesis of RA, the reduction of IL-1ß expression in RA synovial cells is one of mechanisms in reduction of the inflammation in RA by SL.

Effects of Light-Emitting Diode (LED) Irradiation on Blood Serum Components and Inflammatory Cytokines in Various Experimental Models of Diabetic Rats

“…In conclusion, we suggest that abdominal LED irradiation can reduce body weight and LED irradiation could be applicable for use against diabetic-induced inflammatory factors. [8][9][10] ．ま た，低出力レーザー(LLL)および LED の光線照射が， ヒト関節リウマチ動物モデルである collagen 誘発性関節 炎モデルラットの大腿骨・踵骨海綿骨領域の炎症性サイ トカインの遺伝子の発現を抑制して，骨吸収・破壊を抑 制したことを報告した [11][12][13][14] ．さらに，ヒト歯肉線維芽細 胞を用いた in vitro の実験でも，LPS 刺激で上昇した炎症 性サイトカインである PGE 2 ，IL-1β および PA 産生量を いずれも LED 照射により抑制した [15][16][17]…”