2010
DOI: 10.1111/j.1365-3059.2009.02148.x
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Abstract: This work reports the evaluation of citrus canker resistance in transgenic lines of four sweet orange cultivars Hamlin, Natal, Pera and Valencia expressing the rice Xa21 R-gene. The Agrobacterium-mediated genetic transformation was performed and transgene integration was confirmed by Southern blot analyses. These analyses showed that the plants had undergone 1-3 independent transgene integration events. The genetic transformation efficiency was 18AE6% for Hamlin, 8AE3% for Natal, 3% for Pera and 11AE3% for Val… Show more

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Cited by 110 publications
(66 citation statements)
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“…Examples of transgenes or genes whose overexpression confer enhanced resistance are shown in Table 4 [124][125][126][127][128][129][130][131][132][133][134][135][136][137][138]. Nonetheless, a main limitation of the transgenic approach is that adoption of genetically modified plants takes many years because of the rigorous field testing required and the long regulation process.…”
Section: Plant Genetic Resistancementioning
confidence: 99%
“…Examples of transgenes or genes whose overexpression confer enhanced resistance are shown in Table 4 [124][125][126][127][128][129][130][131][132][133][134][135][136][137][138]. Nonetheless, a main limitation of the transgenic approach is that adoption of genetically modified plants takes many years because of the rigorous field testing required and the long regulation process.…”
Section: Plant Genetic Resistancementioning
confidence: 99%
“…Entre as cultivares de laranja doce cultivadas no Brasil, a laranja 'Hamlin' é a mais responsiva, com médias de até 18% de eficiência de transformação genética (BOSCARIOL et al, 2006;MENDES et al, 2010). A laranja 'Pera', principal cultivar destinada ao mercado interno de fruta fresca e para a industrialização , é a menos responsiva.…”
Section: Transformação Genéticaunclassified
“…A partir de então, outros trabalhos envolvendo transformação genética em citros tem sido realizados em diversas espécies e híbridos (PEÑA et al, 2001;YU et al, 2002;BOSCARIOL et al, 2003;AZEVEDO et al, 2006;MOORE, 2008;CARDOSO et al, 2010;MIYATA et al, 2011;MIYATA et al, 2012).…”
Section: Transformação Genética De Citrosunclassified
“…(CARDOSO et al, 2010;BOSCARIOL, et al 2006, MUNIZ et al, 2012. Por outro lado, o uso de promotores tecido-específicos também tem sido relatado, pois, evita uma expressão desnecessária do gene e reduz a possibilidade de interferência no desenvolvimento da planta (DUTT et al, 2012;DUTT et al, 2014;PORTO et al, 2014).…”
Section: Análise De Southern Blotunclassified
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