Reducing systemic absorption and macrophages clearance of genistein by lipid-coated nanocrystals for pulmonary delivery

He, Yuan; Liu, Chang; Han, Run; Liang, Yingmin; Mak, Jcw; Zhu, Yu-Xian; Li, Haifeng; Zheng, Ying

doi:10.1016/j.cclet.2022.04.082

Cited by 5 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After 28-day differentiation, the cells were treated with 1 μg/mL LPS for 12 h to obtain LPS-activated HBECs . After replacing the medium in the lower chamber with 1 mL of HBSS, the LPS-activated cells were treated with 100 μL of DiI labeled lipos, exos, SELs, and D-SELs for 2 h. The FI of different formulations penetrating into the basolateral chamber was detected with a microplate reader (excitation at 549 nm, emission at 565 nm; PerkinElmer, USA) to calculate their corresponding P app values according to a previously developed algorithm . The Transwell chambers were then washed and stained with Hoechst 33342 for the visualization by a CLSM (Leica SP8, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…63 After replacing the medium in the lower chamber with 1 mL of HBSS, the LPS-activated cells were treated with 100 μL of DiI labeled lipos, exos, SELs, and D-SELs for 2 h. The FI of different formulations penetrating into the basolateral chamber was detected with a microplate reader (excitation at 549 nm, emission at 565 nm; PerkinElmer, USA) to calculate their corresponding P app values according to a previously developed algorithm. 64 The Transwell chambers were then washed and stained with Hoechst 33342 for the visualization by a CLSM (Leica SP8, Germany). On the other hand, IL-6, IL-8, and MUC5AC levels in the apical washes from the HBECs were detected by ELISA kits (BioLegend, San Diego, CA), as per the manufacturer's protocol.…”

Section: = × ×mentioning

confidence: 99%

See 1 more Smart Citation

An Inhalable Hybrid Biomimetic Nanoplatform for Sequential Drug Release and Remodeling Lung Immune Homeostasis in Acute Lung Injury Treatment

Liu

et al. 2023

ACS Nano

Self Cite

View full text Add to dashboard Cite

Interactions of lung macrophages and recruited neutrophils with the lung microenvironment continuously aggravate the dysregulation of lung inflammation in the pathogenesis of acute lung injury (ALI) or acute respiratory distress syndrome (ARDS). Either modulating macrophages or destroying neutrophil counts cannot guarantee a satisfactory outcome in ARDS treatment. Aimed at inhibiting the coordinated action of neutrophils and macrophages and modulating the hyper-inflammatory condition, an inhalable biomimetic sequential drug-releasing nanoplatform was developed for the combinatorial treatment of ALI. The nanoplatform (termed D-SEL) was made by conjugating DNase I, as outer cleavable arms, to a serum exosomal and liposomal hybrid nanocarrier (termed SEL) via a matrix metalloproteinase 9 (MMP-9)-cleavable peptide and then encapsulating methylprednisolone sodium succinate (MPS). In lipopolysaccharide (LPS) induced ALI in mice, the MPS/D-SEL moved through muco-obstructive airways and was retained in the alveoli for over 24 h postinhalation. DNase I was then released from the nanocarrier first after responding to MMP-9, resulting in inner SEL core exposure, which precisely delivered MPS into macrophages for promoting M2 macrophage polarization. Local and sustained DNase I release degraded dysregulated neutrophil extracellular traps (NETs) and suppressed neutrophil activation and the mucus plugging microenvironment, which in turn amplified M2 macrophage polarization efficiency. Such dual-stage drug release behavior facilitated down-regulation of pro-inflammatory cytokines in the lung but anti-inflammatory cytokine production through remodeling lung immune homeostasis, ultimately promoting lung tissue repair. This work presents a versatile hybrid biomimetic nanoplatform for the local pulmonary delivery of dual-drug therapeutics and displays potential in the treatment of acute inflammation.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: = × ×mentioning

confidence: 99%

An Inhalable Hybrid Biomimetic Nanoplatform for Sequential Drug Release and Remodeling Lung Immune Homeostasis in Acute Lung Injury Treatment

Liu

et al. 2023

ACS Nano

Self Cite

View full text Add to dashboard Cite

show abstract

“…Inhalation formulations are a class of ultrafine pharmaceutical powders with particle size requirements ranging from 0.5 to 10 μm . As an advanced formulation, it possesses exceptional properties including rapid onset of action, precise targeting, effective dosage, and high activity . These attributes significantly enhance the dissolution and bioavailability of insoluble pharmaceuticals while improving patient compliance and reducing toxicity and side effects to the respiratory tract in treating chronic respiratory diseases and systemic diseases. , The rate of drug deposition in the lungs is a critical factor influencing the efficacy of inhalation formulations.…”

Section: Introductionmentioning

confidence: 99%

A Scalable Freeze-Dissolving Approach to Prepare Ultrafine Crystals for Inhalation: Mechanism and Validation

Guo,

Cao,

Zhang

et al. 2024

Crystal Growth & Design

View full text Add to dashboard Cite

Inhaled drug delivery has garnered increasing attention due to its inherent advantages including rapid onset of action, targeted delivery, high bioavailability, and reduced side effects, which holds promise for the treatment of localized lung diseases and systemic diseases. The currently dominant technologies for the preparation of inhalants are air flow pulverization, media milling, and spray drying, which encounter challenges such as inaccurate particle size control, inefficiency, instability, and difficulties in scaling up. The present study proposes a freezedissolving technique (FDT) for the preparation of inhalable microcrystals, comprising two essential steps: rapid freezing of microdroplets into iced pellets within seconds and subsequent dissolution of a solvent in the iced pellets to release the microcrystals in a suspension. By employing the FDT technique, griseofulvin, a representative inhalant, was successfully engineered into a bipyramidal morphology with a targeted particle size (<5 μm), minimal agglomeration (<3%), and an aspect ratio below 2, in which the solvent and stabilizer systems played pivotal roles in facilitating or impeding nucleation and crystal growth processes. To verify the universality of the FDT, six other typical inhalant preparation processes were successfully developed based on the FDT with a controllable crystal size between 1 and 10 μm. Furthermore, three out of the aforementioned seven model substances were randomly selected, and their preparation was scaled up by a factor of 100, yielding consistent results with those obtained at a smaller test scale. This study outlines the freeze-dissolving mechanism in ultrafine crystal formation and demonstrates that the FDT is a robust and scalable approach for the production of inhalants.

show abstract

Biological Significance of Polyphenols as Functional Molecules in Biomaterial Preparations

Vinayagamurthy,

Kumar,

Yalavarthi

et al. 2024

Science and Engineering of Polyphenols

View full text Add to dashboard Cite

Reducing systemic absorption and macrophages clearance of genistein by lipid-coated nanocrystals for pulmonary delivery

Cited by 5 publications

References 28 publications

An Inhalable Hybrid Biomimetic Nanoplatform for Sequential Drug Release and Remodeling Lung Immune Homeostasis in Acute Lung Injury Treatment

An Inhalable Hybrid Biomimetic Nanoplatform for Sequential Drug Release and Remodeling Lung Immune Homeostasis in Acute Lung Injury Treatment

A Scalable Freeze-Dissolving Approach to Prepare Ultrafine Crystals for Inhalation: Mechanism and Validation

Biological Significance of Polyphenols as Functional Molecules in Biomaterial Preparations

Contact Info

Product

Resources

About