Reduced expression of kinase-associated phosphatase in cortical dendrites of MAP2-deficient mice

Iriuchijima, Nobuhisa; Sato-Harada, Reiko; Takano, Mutsumi; Fujio, Katsunori; Satō, Takashi; Goto, Fumio; Harada, Akira

doi:10.1016/j.bbrc.2005.10.077

Cited by 9 publications

(6 citation statements)

References 16 publications

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“…The absence of HMWMAP2 leads to a reduced amount of dendritic and total PKA and reduced activation of CREB, a transcription factor activated by PKA, in MAP2-deficient mice [Harada et al, 2002]. More recently, expression of the kinase-associated phosphatase (KAP) was shown to be reduced in the cortical dendrites as well as its amount bound to microtubules in MAP2-deficient brains [Iriuchijima et al, 2005]. This study suggested an indirect association between KAP and HMWMAP2 through its interaction with PKA [Iriuchijima et al, 2005].…”

Section: Stabilization and Organization Of Microtubules By Map2 Isoformsmentioning

confidence: 77%

“…More recently, expression of the kinase-associated phosphatase (KAP) was shown to be reduced in the cortical dendrites as well as its amount bound to microtubules in MAP2-deficient brains [Iriuchijima et al, 2005]. This study suggested an indirect association between KAP and HMWMAP2 through its interaction with PKA [Iriuchijima et al, 2005]. HWMMAP2 were also recently shown to link the signaling pathway of the small GTPase Ras to microtubules through an interaction with very-KIND (v-KIND), a novel brain-specific RasGEF preferentially localized in the somato-dendritic compartment [Huang et al, 2007].…”

Section: Stabilization and Organization Of Microtubules By Map2 Isoformsmentioning

confidence: 99%

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Untitled

2008

Cell Motil. Cytoskeleton

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Neuronal polarity is established by the differentiation of two types of cytoplasmic processes: dendrites and the axon. These processes can be distinguished by their composition in microtubule-associated proteins, the high molecular weight MAP2 proteins (HMWMAP2) being found in the dendrites and tau proteins in the axon. It is believed that the main contribution of HMWMAP2 to the acquisition and maintenance of dendrites is to promote microtubule assembly and stability. However, recent studies force us to enlarge our view on how HMWMAP2 might contribute to defining the role of the dendritic microtubules. The purpose of this article is to convey our view that HMWMAP2 are important players in defining the contribution of microtubules to dendritic identity by anchoring membranous organelles and signaling proteins to the dendritic microtubules and by being a receptor for neurosteroids. Cell Motil. Cytoskeleton 65: 515-527, 2008. '

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Section: Stabilization and Organization Of Microtubules By Map2 Isoformsmentioning

confidence: 77%

Section: Stabilization and Organization Of Microtubules By Map2 Isoformsmentioning

confidence: 99%

Untitled

2008

Cell Motil. Cytoskeleton

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“…Diabetes is often associated with increased oxidative stress and low energy levels that contribute to neuron microtubule meshwork degeneration, events that eventually leads to neuron apoptosis 30 – 32 . The up-regulation of several antioxidant and neural protection pathway proteins in the Akita.PlGF −/− compared to Akita conditions is an intriguing and potentially important finding.…”

Section: Discussionmentioning

confidence: 99%

Proteomics reveals ablation of PlGF increases antioxidant and neuroprotective proteins in the diabetic mouse retina

Saddala

Lennikov

Grab

et al. 2018

Sci Rep

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Placental growth factor (PlGF or PGF), a member of the vascular endothelial growth factor (VEGF) sub-family, plays a crucial role in pathological angiogenesis and inflammation. However, the underlying molecular mechanisms that PlGF mediates regarding the complications of non-proliferative diabetic retinopathy (DR) remain elusive. Using an LC-MS/MS-based label-free quantification proteomic approach we characterized the alterations in protein expression caused by PlGF ablation in the retinas obtained from C57BL6, Akita, PlGF−/− and Akita.PlGF−/− mice. After extraction and enzymatic digestion with Trypsin/LysC, the retinal proteins were analyzed by Q-Exactive hybrid Quadrupole-Orbitrap mass spectrometry. Differentially expressed proteins (DEPs) were identified in four comparisons based on Z-score normalization and reproducibility by Pearson’s correlation coefficient. The gene ontology (GO), functional pathways, and protein-protein network interaction analysis suggested that several proteins involved in insulin resistance pathways (Gnb1, Gnb2, Gnb4, Gnai2, Gnao1, Snap2, and Gngt1) were significantly down-regulated in PlGF ablated Akita diabetic mice (Akita.PlGF−/− vs. Akita) but up-regulated in Akita vs. C57 and PlGF−/− vs. C57 conditions. Two proteins involved in the antioxidant activity and neural protection pathways, Prdx6 and Map2 respectively, were up-regulated in the Akita.PlGF−/− vs. Akita condition. Overall, we predict that down-regulation of proteins essential for insulin resistance, together with the up-regulation of antioxidant and neuroprotection proteins highlight and epitomize the potential mechanisms important for future anti-PlGF therapies in the treatment of DR.

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“…Furthermore Map2 also has a binding domain for the regulatory subunit II of cAMP-dependent protein kinase (PKA). It is known that in diabetes increased oxidative stress and low energy levels contribute to neuron microtubule meshwork degeneration that eventually leads to neuron cell death (Harada et al, 2002; Iriuchijima et al, 2005; Teng et al, 2001). Our results revealed that Map2 protein is up-regulated in Akita.P1GF −/− compared to Akita mice retinas.…”

Section: Discussionmentioning

confidence: 99%

Proteomics reveals ablation of placental growth factor inhibits the insulin resistance pathways in diabetic mouse retina

Lennikov

Tang³

et al. 2018

Preprint

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The authors wish to acknowledge the contribution of: Lijuan Fan for technical assistance, Dmitry Rumyancev for 26 artwork design, and Jianjiang Hao for MS analysis. Abstract 41The underlying molecular mechanisms that placental growth factor (PlGF) mediates the early complications at non-42 proliferative diabetic retinopathy (DR) remain largely elusive. The objective of this study is to characterize 43 expression profile due to PlGF ablation in the retina of diabetic mice. The quantitative label-free proteomics was 44 carried out on retinal tissues collected from mouse strains (Akita; PlGF -/and Akita.PlGF -/-). We have identified 45 3176 total proteins, and 107 were significantly different between the experimental groups, followed by gene 46 ontology, functional pathways, and protein-protein network interaction analysis. Gnb1, Gnb2, Gnb4, Gnai2, Gnao1, 53 Total words: 175 54 55 56 57 58

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Reduced expression of kinase-associated phosphatase in cortical dendrites of MAP2-deficient mice

Cited by 9 publications

References 16 publications

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Proteomics reveals ablation of PlGF increases antioxidant and neuroprotective proteins in the diabetic mouse retina

Proteomics reveals ablation of placental growth factor inhibits the insulin resistance pathways in diabetic mouse retina

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