Redox potential of FAD-dependent glucose dehydrogenase

Schachinger, Franziska; Ma, Su; Ludwig, Roland

doi:10.1016/j.elecom.2022.107405

Cited by 2 publications

(2 citation statements)

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“…Gold electrodes were electrochemically cleaned following the procedure described in the previous study [44] . After that, the gold working electrodes were then used for the self‐assembly immobilization of 10 mM thioglycerol in absolute ethanol overnight.…”

Section: Methodsmentioning

confidence: 99%

“…Gold electrodes were electrochemically cleaned following the procedure described in the previous study. [44] After that, the gold working electrodes were then used for the self-assembly immobilization of 10 mM thioglycerol in absolute ethanol overnight. Branched-PEI (Mw = 750 kDa, pH~10) was prepared and dissolved in Milli-Q water to the concentration of 1 or 10 μM and contained 100 mM KCl as a supporting electrolyte.…”

Section: Preparation Of Cdh-pei Layers On Gold Electrodesmentioning

confidence: 99%

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Electron Transfer of Cellobiose Dehydrogenase in Polyethyleneimine Films

Karnpakdee,

Kracher,

Ludwig

2024

ChemElectroChem

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Cellobiose dehydrogenase (CDH) is applied as a bioelectrocatalyst in biosensors because its mobile cytochrome domain is capable of direct electron transfer. This study investigates the electron transfer mechanism of CDH molecules embedded in the polycation polyethyleneimine (PEI), which has been reported as a current‐boosting component of CDH‐based biosensors. By immobilizing different concentrations of CDH and its isolated cytochrome domain in PEI films, we found that increasing concentrations of cytochrome enhanced the film conductivity (up to 251±8 mS cm−1) through improved electron transfer between the protein redox centers. The increased electrical conductivity of the film contacts CDH molecules at a greater distance from the electrode. The cross‐linker poly(ethylene glycol) diglycidyl ether improves the packing and contacting of the cytochrome domains, whereas glutaraldehyde reduces the current obtained. Deglycosylation of CDH enhances the conductivity of enzyme‐polymer films by up to 34 %, implying a higher number of productive electron‐hopping events between cytochrome domains due to enhanced mobility or reduced shielding. By balancing negative charges on the CDH surface at neutral and alkaline pH, PEI increases the interdomain electron transfer and the electrical film conductivity. The resulting increased current output is relevant for in vivo bioanalytical applications.

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Section: Methodsmentioning

confidence: 99%

Section: Preparation Of Cdh-pei Layers On Gold Electrodesmentioning

confidence: 99%