Redox Parameters as Markers of the Rate of Aging and Predictors of Life Span

Toda, Irene Martínez de; Vida, Carmen; Garrido, António; Fuente, Mónica De la

doi:10.1093/gerona/glz033

Cited by 26 publications

(42 citation statements)

References 49 publications

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“…Then, they were sonicated and supernatants were used for the enzymatic reaction together with 14 mM H 2 O 2 as substrate. Decomposition of H 2 O 2 was measured at 240 nm as previously described [12]. The results were expressed as units (U) of catalase activity/mg protein.…”

Section: Methodsmentioning

confidence: 99%

“…Cell suspensions were adjusted to 10 6 total cells/mL in Hank's medium and centrifuged, and the cell pellets were resuspended in oxygen-free phosphate buffer 50 mM and EDTA 6.3 mM. Then, they were sonicated and supernatants were used for the enzymatic reaction together with oxidized glutathione (GSSG) 80 mM as substrate, as previously described [12]. Oxidation of NADPH was measured at 340 nm.…”

Section: Methodsmentioning

confidence: 99%

“…Cell suspensions were adjusted to 10 6 total cells/mL in Hank's medium and centrifuged, and cell pellets were resuspended in phosphate buffer 50 mM and EDTA 0.1 M, pH 8. Then, they were sonicated and supernatants were used for the quantification of both reduced (GSH) and oxidized (GSSG) glutathione by the reaction capacity that GSSG and GSH have with o-phthalaldehyde (OPT) at pH 12 and pH 8, respectively, resulting in the formation of a fluorescent compound, as previously described [12]. Fluorescence was measured at 350 nm excitation and 420 nm emission.…”

Section: Methodsmentioning

confidence: 99%

“…After 60 min incubation, the reaction was stopped with HCl 0.5 M; samples were centrifuged and supernatants discarded. Intracellular reduced NBT was extracted with dioxan and absorbance was measured at 525 nm, as previously described [12]. Results were expressed as nmol superoxide anion/10 6 macrophages.…”

Section: Methodsmentioning

confidence: 99%

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Function, Oxidative, and Inflammatory Stress Parameters in Immune Cells as Predictive Markers of Lifespan throughout Aging

Toda

Vida

Miguel

et al. 2019

Oxidative Medicine and Cellular Longevity

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According to the oxidative-inflammatory theory of aging, there is a link between the function, the oxidative-inflammatory stress state of immune cells, and longevity. However, it is unknown which immune cell parameters can predict lifespan and if there would be any changes in this prediction, depending on the age of the subject. Therefore, a longitudinal study in mice was performed analysing immune function (chemotaxis of macrophages and lymphocytes, phagocytosis of macrophages, natural killer (NK) activity, and lymphoproliferation capacity), antioxidant (catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) activities as well as reduced glutathione (GSH) concentrations), oxidant (oxidized glutathione (GSSG), superoxide anion, and malondialdehyde (MDA) concentrations), and inflammation-related markers (basal release of IL-1β, IL-6, TNF-α, and IL-10) in peritoneal leukocytes from mice at the adult, mature, old, very old, and long-lived ages (40, 56, 72, 96, and 120±4 weeks of age, respectively). The results reveal that some of the investigated parameters are determinants of longevity at the adult age (lymphoproliferative capacity, lymphocyte chemotaxis, macrophage chemotaxis and phagocytosis, GPx activity, and GSH, MDA, IL-6, TNF-α, and IL-10 concentrations), and therefore, they could be proposed as markers of the rate of aging. However, other parameters are predictive of extreme longevity only at the very old age (NK activity, CAT and GR activities, and IL-6 and IL-1β concentrations), and as such, they could reflect some of the adaptive mechanisms underlying the achievement of high longevity. Nevertheless, although preliminary, the results of the present study provide a new perspective on the use of function, redox, and inflammatory parameters in immune cells as prognostic tools in aging research and represent a novel benchmark for future work aimed at prediction of lifespan.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Function, Oxidative, and Inflammatory Stress Parameters in Immune Cells as Predictive Markers of Lifespan throughout Aging

Toda

Vida

Miguel

et al. 2019

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

show abstract

“…cross-sectional and mixed-longitudinal studies [69,70,86,[98][99][100][101][102], but not always [23,55,[103][104][105][106]. For example, urinary 8-OHdG increased with age in all patients with muscular dystrophy [86] and between certain age categories in a large cross-sectional sample of men and women [100], but did not increase with age in pollution-exposed silica miners [106].…”

Section: Plos Onementioning

confidence: 99%

Urinary markers of oxidative stress respond to infection and late-life in wild chimpanzees

et al. 2020

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Oxidative stress (OS) plays a marked role in aging and results from a variety of stressors, making it a powerful measure of health and a way to examine costs associated with life history investments within and across species. However, few urinary OS markers have been examined under field conditions, particularly in primates, and their utility to non-invasively monitor the costs of acute stressors versus the long-term damage associated with aging is poorly understood. In this study, we examined variation in 5 urinary markers of oxidative damage and protection under 5 validation paradigms for 37 wild, chimpanzees living in the Kibale National Park, Uganda. We used 924 urine samples to examine responses to acute immune challenge (respiratory illness or severe wounding), as well as mixed-longitudinal and intra-individual variation with age. DNA damage (8-OHdG) correlated positively with all other markers of damage (F-isoprostanes, MDA-TBARS, and neopterin) but did not correlate with protection (total antioxidant capacity). Within individuals, all markers of damage responded to at least one if not both types of acute infection. While OS is expected to increase with age, this was not generally true in chimpanzees. However, significant changes in oxidative damage were detected within past-prime individuals and those close to death. Our results indicate that OS can be measured using field-collected urine and integrates short-and long-term aspects of health. They further suggest that more data are needed from long-lived, wild animals to illuminate if common age-related increases in inflammation and OS damage are typical or recently aberrant in humans.

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The application prospect of metal/metal oxide nanoparticles in the treatment of osteoarthritis

Luo

Zhang

Zhu

et al. 2021

Naunyn-Schmiedeberg's Arch Pharmacol

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The current understanding of osteoarthritis is developing from a mechanical disease caused by cartilage wear to a complex biological response involving inflammation, oxidative stress and other aspects. Nanoparticles are widely used in drug delivery due to its good stability in vivo and cell uptake efficiency. In addition to the above advantages, metal/metal oxide NPs, such as cerium oxide and manganese dioxide, can also simulate the activity of antioxidant enzymes and catalyze the degradation of superoxide anions and hydrogen peroxide. Degrading of metal/metal oxide nanoparticles releases metal ions, which may slow down the progression of osteoarthritis by inhibiting inflammation, promoting cartilage repair and inhibiting cartilage ossification. In present review, we focused on recent research works concerning osteoarthritis treating with metal/metal oxide nanoparticles, and introduced some potential nanoparticles that may have therapeutic effects.

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Redox Parameters as Markers of the Rate of Aging and Predictors of Life Span

Cited by 26 publications

References 49 publications

Function, Oxidative, and Inflammatory Stress Parameters in Immune Cells as Predictive Markers of Lifespan throughout Aging

Function, Oxidative, and Inflammatory Stress Parameters in Immune Cells as Predictive Markers of Lifespan throughout Aging

Urinary markers of oxidative stress respond to infection and late-life in wild chimpanzees

The application prospect of metal/metal oxide nanoparticles in the treatment of osteoarthritis

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