Redox compounds influence on the NAD(P)H:FMN—oxidoreductase—luciferase bioluminescent system

Vetrova, E. V.; Kudryasheva, Nadezhda S.; Kratasyuk, Valentina A.

doi:10.1039/b608152e

Cited by 37 publications

(46 citation statements)

References 20 publications

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“…NADH, organic reducer, is a component of many biochemical reactions including those of the bacterial bioluminescent enzyme system [44]. Change of rate of NADH oxidation under influence of exogenous compounds serves as an indicator of intensification (or slowdown) of redox processes in the assay system.…”

Section: Effects Of Radionuclides On Endogenous Reducer Nadhmentioning

confidence: 99%

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Rozhko

Devyatlovskaya

Kudryasheva³

2014

Open Life Sciences

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Effects of Americium-241 (241Am), alpha-emitting radionuclide of high specific radioactivity, and tritium (3H), beta-emitting radionuclide, on luminous bacteria Photobacterium phosphoreum were compared. Bioluminescence intensity served as a marker of bacterial physiological activity. Three successive stages in the bioluminescence response to 241Am and 3H were found under conditions of lowdose irradiation: (1) absence of effects, (2) activation, and (3) inhibition. They were interpreted in terms of bacterial response to stressfactor as stress recognition, adaptive response/syndrome, and suppression of physiological function (i.e. radiation toxicity). Times of bioluminescence activation (TBA) and inhibition (TBI) were suggested as parameters to characterize hormesis and toxic stages in a course of chronic low-dose irradiation of the microorganisms. Values of TBA and TBI of 241Am were shorter than those of 3H, revealing higher impact of alpha-irradiation (as compared to beta-irradiation) under comparable radiation doses. Increases of peroxide concentration and NADH oxidation rates in 241Am aquatic solutions were demonstrated; these were not found in tritiated water. The results reveal a biological role of reactive oxygen species generated in water solutions as secondary products of the radioactive decay. The study provides a scientific basis for elaboration of bioluminescence-based assay to monitor radiotoxicity of alpha- and beta-emitting radionuclides in aquatic solutions.

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Section: Effects Of Radionuclides On Endogenous Reducer Nadhmentioning

confidence: 99%

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Rozhko

Devyatlovskaya

Kudryasheva³

2014

Open Life Sciences

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“…In this case, the delay period appears in bioluminescent kinetics. In Kudryasheva et al [18] et al [26], the bioluminescent enzymatic assay system was already used to monitor oxidative toxicity of organic oxidizers, quinones. Figure 1 presents schematically the bioluminescence kinetics in the presence and absence of the organic oxidizers according Kudryasheva et al [18].…”

Section: Introductionmentioning

confidence: 99%

Effect of humic substances on toxicity of inorganic oxidizer bioluminescent monitoring

Tarasova

Стом

Kudryasheva

2011

Enviro Toxic and Chemistry

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The current study deals with the effect of humic substances (HS) on toxicity of solutions of a model inorganic oxidizer, potassium ferricyanide. Chemical reactions responsible for toxicity changes are under consideration. The bioluminescent system of coupled enzymatic reactions catalyzed by bacterial luciferase and oxidoreductase was used as a bioassay. General and oxidative toxicity of ferricyanide solutions were evaluated. Ability of HS to decrease or increase general and oxidative toxicity of the solutions was revealed. Two types of chemical processes are supposed to be responsible for detoxification by HS: ferricyanide-HS complex formation and acceleration of endogenous redox reactions in the bioluminescent assay system. Decrease of oxidative toxicity of ferricyanide solution was observed under incubation with HS at all concentrations of HS used. Conditions for general toxicity decrease were prior incubation of ferricyanide with HS and low HS concentrations (< 10⁻⁴g/L). Acceleration of NADH auto-oxidation under higher HS concentrations was supposed to result in a toxicity increase.

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“…Oxidative toxicity is attributed to redox activity of toxic compounds, while the general toxicity (GT) integrates, in a nonadditive way, all the interactions of toxic compounds with components of the bioluminescent enzymatic system, that is redox, hydrophobic and polar interactions. Using the bioluminescent enzymatic assay, the general and oxidative toxicities of organic and inorganic oxidizer' solutions, quinones and polyvalent metals, have been previously studied (25)(26)(27). A decrease of both general and oxidative toxicities under exposure to humic substances, the products of natural decomposition of organic matter in soil and bottom sediments, has been previously demonstrated (28)(29)(30).…”

Section: Introductionmentioning

confidence: 99%

“…The possibility of studying the physicochemical and molecular mechanisms of toxic effects is a benefit of the enzymatic assay. The physicochemical classification of the effects of toxic compounds on bioluminescent enzyme system was suggested by Kudryasheva (21) and developed in (22)(23)(24)(25).…”

Section: Introductionmentioning

confidence: 99%

Bioluminescent Enzymatic Assay as a Tool for Studying Antioxidant Activity and Toxicity of Bioactive Compounds

Kudryasheva

Kovel

Sachkova

et al. 2016

Photochem & Photobiology

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A bioluminescent assay based on a system of coupled enzymatic reactions catalyzed by bacterial luciferase and NADH: FMN-oxidoreductase was developed to monitor toxicity and antioxidant activity of bioactive compounds. The assay enables studying toxic effects at the level of biomolecules and physicochemical processes, as well as determining the toxicity of general and oxidative types. Toxic and detoxifying effects of bioactive compounds were studied. Fullerenols, perspective pharmaceutical agents, nanosized particles, water-soluble polyhydroxylated fullerene-60 derivatives were chosen as bioactive compounds. Two homologous fullerenols with different number and type of substituents, C 60 O 2-4 (OH) [20][21][22][23][24] and Fe 0.5 C 60 (OH) x O y (x + y = 40-42), were used. They suppressed bioluminescent intensity at concentrations >0.01 g L À1 and >0.001 g L À1 for C 60 O 2-4 (OH) 20-24 and Fe 0.5 C 60 (OH) x O y , respectively; hence, a lower toxicity of C 60 O 2-4 (OH) 20-24 was demonstrated. Antioxidant activity of fullerenols was studied in model solutions of organic and inorganic oxidizers; changes in toxicities of general and oxidative type were determined; detoxification coefficients were calculated. Fullerenol C 60 O 2-4 (OH) 20-24 revealed higher antioxidant ability at concentrations 10 À17 À10 À5 g L À1 . The difference in the toxicity and antioxidant activity of fullerenols was explained through their electron donor/acceptor properties and different catalytic activity. Principles of bioluminescent enzyme assay application for evaluating the toxic effect and antioxidant activity of bioactive compounds were summarized.

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Redox compounds influence on the NAD(P)H:FMN—oxidoreductase—luciferase bioluminescent system

Cited by 37 publications

References 20 publications

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria

Effect of humic substances on toxicity of inorganic oxidizer bioluminescent monitoring

Bioluminescent Enzymatic Assay as a Tool for Studying Antioxidant Activity and Toxicity of Bioactive Compounds

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