Red fluorescent genetically encoded indicator for intracellular hydrogen peroxide

Ermakova, Yulia G.; Bilan, Dmitry S.; Matlashov, Mikhail E.; Mishina, Natalia M.; Markvicheva, Ksenia N.; Subach, Fedor V.; Bogeski, Ivan; Hoth, Markus; Enikolopov, Grigori; Belousov, Vsevolod V.

doi:10.1038/ncomms6222

Cited by 222 publications

(217 citation statements)

References 48 publications

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“…The change in fluorescence is induced by a redox-dependent conformational change within the protein [101]. Differently modified forms of HyPer are available such as HyPerRed, which emits in the red spectral region [104] and several are commercially available. HyPer allows for ratiometric measurements, it is fully reversible and can be modified to target specific subcellular compartments [55].…”

Section: Genetically Encoded Probes-fluorescent Proteinsmentioning

confidence: 99%

Possibilities and Challenges for Quantitative Optical Sensing of Hydrogen Peroxide

2017

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Abstract:Hydrogen peroxide (H 2 O 2 ) plays a key role in many biological processes spanning from coral bleaching, over cell signaling to aging. However, exact quantitative assessments of concentrations and dynamics of H 2 O 2 remain challenging due to methodological limitationsespecially at very low (sub µM) concentrations. Most published optical detection schemes for H 2 O 2 suffer from irreversibility, cross sensitivity to other analytes such as other reactive oxygen species (ROS) or pH, instability, temperature dependency or limitation to a specific medium. We review optical detection schemes for H 2 O 2 , compare their specific advantages and disadvantages, and discuss current challenges and new approaches for quantitative optical H 2 O 2 detection, with a special focus on luminescence-based measurements. We also review published concentration ranges for H 2 O 2 in natural habitats, and physiological concentrations in different biological samples to provide guidelines for future experiments and sensor development in biomedical and environmental science.

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Section: Genetically Encoded Probes-fluorescent Proteinsmentioning

confidence: 99%

Possibilities and Challenges for Quantitative Optical Sensing of Hydrogen Peroxide

2017

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“…Several chemical probes have been developed to investigate redox mechanisms, mainly composed of a fluorophore that emits or is liberated on oxidation (351). More recently, genetically encoded fluorescent redox sensors or injected exomarkers allow the detection and visualization of live variations of ROS or redox-regulated molecules (e.g., GSH, NAD) both in vitro and in vivo (upper right) (5,68,98,197). ROSinduced PTMs have recently been described due to the development of redox proteomics, thanks to the setup of cysteine residue labeling (bottom right).…”

Section: Fig 15mentioning

confidence: 99%

Redox Control of Skeletal Muscle Regeneration

Moal

Pialoux

Juban

et al. 2017

Antioxidants & Redox Signaling

148

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Skeletal muscle shows high plasticity in response to external demand. Moreover, adult skeletal muscle is capable of complete regeneration after injury, due to the properties of muscle stem cells (MuSCs), the satellite cells, which follow a tightly regulated myogenic program to generate both new myofibers and new MuSCs for further needs. Although reactive oxygen species (ROS) and reactive nitrogen species (RNS) have long been associated with skeletal muscle physiology, their implication in the cell and molecular processes at work during muscle regeneration is more recent. This review focuses on redox regulation during skeletal muscle regeneration. An overview of the basics of ROS/RNS and antioxidant chemistry and biology occurring in skeletal muscle is first provided. Then, the comprehensive knowledge on redox regulation of MuSCs and their surrounding cell partners (macrophages, endothelial cells) during skeletal muscle regeneration is presented in normal muscle and in specific physiological (exercise-induced muscle damage, aging) and pathological (muscular dystrophies) contexts. Recent advances in the comprehension of these processes has led to the development of therapeutic assays using antioxidant supplementation, which result in inconsistent efficiency, underlying the need for new tools that are aimed at precisely deciphering and targeting ROS networks. This review should provide an overall insight of the redox regulation of skeletal muscle regeneration while highlighting the limits of the use of nonspecific antioxidants to improve muscle function. Antioxid. Redox Signal. 27, 276-310.

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“…These newer probes offer sensitive and more reliable readouts but are difficult to apply to some tissues like leaves, and their specificity is still disputed (Winterbourn, 2014). Genetically encoded sensors such as the ratiometric HyPer and HyPerRed were also used for ROS and redox detection in previous studies (Chiu et al, 2014;Ermakova et al, 2014). A major advantage for future plant ROS research would be to standardize protocols for in vivo cell imaging.…”

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confidence: 99%

Recent Progress in Understanding the Role of Reactive Oxygen Species in Plant Cell Signaling

2016

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Red fluorescent genetically encoded indicator for intracellular hydrogen peroxide

Cited by 222 publications

References 48 publications

Possibilities and Challenges for Quantitative Optical Sensing of Hydrogen Peroxide

Possibilities and Challenges for Quantitative Optical Sensing of Hydrogen Peroxide

Redox Control of Skeletal Muscle Regeneration

Recent Progress in Understanding the Role of Reactive Oxygen Species in Plant Cell Signaling

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