Red cell alloimmunization in multitransfused patients and multiparous women

Abstract

Purpose To determine the incidence of alloimmunization against red cell antigens and the thermal amplitude and specifi city of antibodies in multitransfused patients and multiparous women.Methods Antibody screening was performed in 30 nontransfused orthopedic surgery cases (control), 504 multitransfused patients and 325 multiparous women. Antibody screening at 4°C, 22°C and 37°C was carried out in a saline phase, by indirect antiglobulin technique (IAT), using papain cystein, low ionic strength solution (LISS) and polyethylene glycol (PEG). ResultsIn multitransfused patients IgM antibodies were more frequently detected at 4°C and the IgG antibody incidence was 7.1% by enzyme method, 7.7% IAT, 9.4% LISS, 10.2% using PEG & 10.2% multiparous women. Bad obstetric history cases had signifi cantly higher incidence of alloimmunization. The antibody specifi city of antibodies was mainly in Lewis, Rh, Kidd and MN systems.Conclusion Antibody screening before transfusion, at set time intervals after transfusion and during antenatal period is recommended.

Red Cell Alloimmunization in Repeatedly Transfused Chronic Renal Failure Patients in Jaipur.

“…Thereby, antibodies arise in the chronic renal failure patients after exposure to multiple of red blood cell transfusions. 4 We observed of alloimmunization prevalence was 18 % detected in 7 out of 39 CRF patients, this finding is higher than the frequency of previous studies have done in Sudan country that they reported of prevalence 13.1 % 4 , Similarly study by Domen and Ramirez showed the rate 6.1 % 10 and frequency of 9.9 % reported by Shukla 2 in CRF patients undergoing dialysis while study by Patel et al 11 had been shown that the alloimmunization rate 8.2 % in CRF patients.…”

Frequency of Alloantibodies Among Chronic Renal Failure Patients in Red Sea State

“…Other study from Sharkia reported alloimmunization rate of 9.5 % in multiple transfused patients [14] while study by Patel et al [15] had been shown that the alloimmunization rate 8.2 % in CRF patients. The differences in the alloinummization rate were attributed to: Heterogeneity among the population, in pre-transfusion test blood were matched only for ABO and Rh(D)antigens, sample size of study population and sensitivity of the test method .…”

Red cell alloimmunization in multitransfused chronic renal patients on haemodialysis in a tertiary care centre of Jammu region

“…10 There have been a few studies from different parts of world that have demonstrated the presence of alloantibodies in thalassemia patients, Sickle cell disease patients, and also chronic renal disease patients. 7,8,14 This study was undertaken to determine the frequency and specificity of RBC alloantibodies among the chronic renal failure patients of Jammu region which have not been previously described.…”

“…Red blood cell alloantibodies can develop in approximately 6-36% of patients who receive repeated transfusion. 7,8 Various factors influence alloantibody formation in our body including the number and frequency of the transfusions as well as the recipient's sex, age, immune status and underlying disease. [9][10][11] The antibodies thus formed result in difficulty in cross matching, various types of transfusion reactions, haemolysis and other potentially life-threatening events.…”

“…The reports have demonstrated varying percentages in different population groups and different diseases. 7,8,13,14 Keeping in view this disparity this study was planned and conducted to identify alloantibodies in patients of chronic renal failure undergoing dialysis at a tertiary care center in northern India.…”