Red Blood Cells Protein Profile Is Modified in Breast Cancer Patients

Pereira-Veiga, Thais; Bravo, Susana B.; Gómez-Tato, Antonio; Yáñez-Gómez, Celso; Abuín, Carmen; Pose, V. Varela; Cueva, Juan; Palacios, Patricia; Dávila-Ibáñez, Ana B.; Piñeiro, Roberto; Vilar, Ana; Chantada-Vázquez, María Del Pilar; López‐López, Rafael; Costa, Clotilde

doi:10.1016/j.mcpro.2022.100435

Cited by 9 publications

(9 citation statements)

References 78 publications

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“…In clinics, RDW is often used for the auxiliary diagnosis of anemia. In recent years, more and more studies have found that RDW plays an essential role in the treatment of cardiovascular diseases, 14 venous thromboembolism, 15 cancer, 16 diabetes, 17 and lung disease. 18 Studies have shown that a combination of baseline RDW values and increased RDW can predict adverse outcomes in patients with sepsis, 19 supporting this study's results.…”

Section: Discussionmentioning

confidence: 99%

Immune Dysfunction-Associated Elevated RDW, APACHE-II, and SOFA Scores Were a Possible Cause of 28-Day Mortality in Sepsis Patients

Wang,

He,

Jin

et al. 2024

IDR

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To explore the early predictors and their predicting value of 28-day mortality in sepsis patients and to investigate the possible causes of death. Methods: 127 sepsis patients were included, including 79 cases in the survival group and 48 cases in the death group. The results of all patients on admission were recorded. After screening the risk factors of 28-day mortality, the receiver operating characteristic curve (ROC) was used to determine their predictive value for the 28-day mortality rate on admission, and the Kaplan-Meier curve was drawn to compare the 28-day mortality rate between groups. Finally, patients with cytokine and lymphocyte subsets results were included for investigating the possible causes of death through correlation analysis. Results: APACHE II (acute physiology and chronic health evaluation II), SOFA (Sequential Organ Failure Assessment) and red blood cell distribution width (RDW) were the risk factors for 28-day mortality in sepsis patients (OR: 1.130 vs.1.160 vs.1.530, P < 0.05). The area under the curve (AUC), sensitivity and specificity of APACHE II, SOFA and RDW in predicting the mortality rate at 28 days after admission in sepsis patients were 0.763 vs 0.806 vs 0.723, 79.2% vs 68.8% vs 75.0%, 65.8% vs 89.9% vs 68.4%. The combined predicted AUC was 0.873, the sensitivity was 89.6%, and the specificity was 82.3%. The Kaplan-Meier survival curve showed that the 28-day mortality rates of sepsis patients with APACHE II≥18.5, SOFA≥11.5 and RDW≥13.8 were 58.5%, 80.5% and 59.0%, respectively. In the death group, APACHE II was positively correlated with SOFA, IL-2, and IL-10, and RDW was positively correlated with PLT, TNF-α, CD3 + lymphocyte count, and CD8 + lymphocyte count. Conclusion: Sepsis patients with high APACHE II, SOFA and RDW levels at admission have an increased 28-day mortality rate. The elevation of these indicators in dead patients are related to immune dysfunction.

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Section: Discussionmentioning

confidence: 99%

Immune Dysfunction-Associated Elevated RDW, APACHE-II, and SOFA Scores Were a Possible Cause of 28-Day Mortality in Sepsis Patients

Wang,

He,

Jin

et al. 2024

IDR

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“…Protein Digestion: For protein identification, equal amounts of protein from each femur sample (n = 4 per group) were loaded onto a 10% SDS-PAGE gel. The resulting condensed protein bands [ 76 , 77 ] underwent gel digestion using Trypsin [ 78 ] and were processed as described in the Supplementary Materials and Methods and previously by our group [ 79 ].…”

Section: Methodsmentioning

confidence: 99%

“…LC-MSMS in Data-Dependent Acquisition Mode, Shotgun Analysis, and Spectral Count Transformation: Digested peptides from each mouse femur sample were separated using reverse phase chromatography as described previously [ 79 ] and in the Supplementary Materials and Methods . Semi-quantification by spectral counting was performed using Scaffold software (version 5.0.1; Proteome Software Inc., Portland, OR, USA), as described previously [ 80 ].…”

Section: Methodsmentioning

confidence: 99%

Morquio A Syndrome: Identification of Differential Patterns of Molecular Pathway Interactions in Bone Lesions

Álvarez,

Bravo,

Chantada-Vázquez

et al. 2024

IJMS

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Mucopolysaccharidosis type IVA (MPS IVA; Morquio A syndrome) is a rare autosomal recessive lysosomal storage disease (LSD) caused by deficiency of a hydrolase enzyme, N-acetylgalactosamine-6-sulfate sulfatase, and characterized clinically by mainly musculoskeletal manifestations. The mechanisms underlying bone involvement in humans are typically explored using invasive techniques such as bone biopsy, which complicates analysis in humans. We compared bone proteomes using DDA and SWATH-MS in wild-type and MPS IVA knockout mice (UNT) to obtain mechanistic information about the disease. Our findings reveal over 1000 dysregulated proteins in knockout mice, including those implicated in oxidative phosphorylation, oxidative stress (reactive oxygen species), DNA damage, and iron transport, and suggest that lactate dehydrogenase may constitute a useful prognostic and follow-up biomarker. Identifying biomarkers that reflect MPS IVA clinical course, severity, and progression have important implications for disease management.

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“…Although these associated cells lack carcinogenic mutations, their phenotype undergoes changes that contribute to promoting tumor progression ( 116 ). It has been recently reported that red blood cells (RBCs) that accompany CTCs have prognostic value in MBC patients ( 117 ) and acquire modifications in their proteomic composition that were not observed among healthy donors ( 118 ). The lysosome-associated membrane glycoprotein 2 (LAMP2) was overexpressed in RBCs and high levels were associated with worse outcomes in MBC patients, suggesting that alterations in non-malignant components may provide additional information next to CTCs, though these are preliminary results and further studies are needed.…”

Section: Ctcs and Circulating Non-malignant Cellsmentioning

confidence: 99%

Circulating tumor cells et al.: towards a comprehensive liquid biopsy approach in breast cancer

Nicolò,

Gianni,

Pontolillo

et al. 2024

Transl Breast Cancer Res

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Liquid biopsy has emerged as a crucial tool in managing breast cancer (BC) patients, offering a minimally invasive approach to detect circulating tumor biomarkers. Until recently, the majority of the studies in BC focused on evaluating a single liquid biopsy analyte, primarily circulating tumor DNA and circulating tumor cells (CTCs). Despite the proven prognostic and predictive value of CTCs, their low abundance when detected using enrichment methods, especially in the early stages, poses a significant challenge. It is becoming evident that combining diverse circulating biomarkers, each representing different facets of tumor biology, has the potential to enhance the management of patients with BC. This article emphasizes the importance of considering these biomarkers as complementary/synergistic rather than competitive, recognizing their ability to contribute to a comprehensive disease profile. The review provides an overview of the clinical significance of simultaneously analyzing CTCs and other biomarkers, including cell-free circulating DNA, extracellular vesicles, non-canonical CTCs, cell-free RNAs, and non-malignant cells. Such a comprehensive liquid biopsy approach holds promise not only in BC but also in other cancer types, offering opportunities for early detection, prognostication, and therapy monitoring. However, addressing associated challenges, such as refining detection methods and establishing standardized protocols, is crucial for realizing the full potential of liquid biopsy in transforming our understanding and approach to BC. As the field evolves, collaborative efforts will be instrumental in unlocking the revolutionary impact of liquid biopsy in BC research and management.

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Red Blood Cells Protein Profile Is Modified in Breast Cancer Patients

Cited by 9 publications

References 78 publications

Immune Dysfunction-Associated Elevated RDW, APACHE-II, and SOFA Scores Were a Possible Cause of 28-Day Mortality in Sepsis Patients

Immune Dysfunction-Associated Elevated RDW, APACHE-II, and SOFA Scores Were a Possible Cause of 28-Day Mortality in Sepsis Patients

Morquio A Syndrome: Identification of Differential Patterns of Molecular Pathway Interactions in Bone Lesions

Circulating tumor cells et al.: towards a comprehensive liquid biopsy approach in breast cancer

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