volume 107, issue 10, P2825-2837 1994
DOI: 10.1242/jcs.107.10.2825
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I. Lajoie-Mazenc, Y. Tollon, C. Detraves, M. Julian, A. Moisand, C. Gueth-Hallonet, A. Debec, I. Salles-Passador, A. Puget, H. Mazarguil

Abstract: It has been claimed repeatedly that gamma-tubulin is exclusively localized at the spindle poles in mitotic animal cells, where it plays a role in microtubule nucleation. In addition to this localization, we have observed a gamma-tubulin-specific staining of the mitotic spindle in several animal cells (human, kangaroo rat, mouse, Chinese hamster, Xenopus and Drosophila) using five polyclonal antibodies raised against unique gamma-tubulin sequences and four different fixation protocols. In HeLa and PtK2 cells, g…

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