“…cytoplasm, cytoplasmic organelles, nuclear soluble and chromatin. Proteins were detected by Western blotting [14] using in house (anti-WRN, -RECQL4, -RECQL5), Abcam (anti-CtIP, -XLF), Millipore (histone 3), BD Pharmingen (anti-53BP1, -calretuculin, -Top1) and Santa Cruz (anti-RECQL1, -Ku80, -actin, -tubulin) antibodies and quantitated by using ImageJ 1.46r software (National Institutes of Health, USA).…”