Abstract:We reported previously that egg membrane rafts serve as a subcellular microdomain for sperm-dependent tyrosine kinase signaling in Xenopus fertilization. Moreover, we demonstrated that raft-associated Src tyrosine kinase was activated by sperm in vitro. Here we show that egg rafts incubated with sperm or hydrogen peroxide (H 2 O 2 ) can promote Src-dependent phosphorylation of phospholipase C␥ (PLC␥) and transient calcium release in the extracts of unfertilized Xenopus eggs. In vivo egg activation by sperm or … Show more
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