Recommendations for calculating growth parameters by optical density measurements

Biological Toxicities and Aggregation Effects of ʟ-Glycine and ʟ-Alanine Capped ZnS:Mn Nanocrystals in Aqueous Solution

“…For these experiments, the optical density (OD) 600 method was employed, which measures the degree of turbidity of the nutrient broth by filling with growing bacteria. 30 The more turbid solution scatters more scanned light, so that the intensities of the passing light decreases with the bacterial growth. Usually, spectroscopic bacterial growth monitoring is performed at a working range of 550-600 nm, because the nutrient broth has a very low OD value at this wavelength region.…”

Ability of a Lactobacillus rhamnosus strain cultured in milk whey based medium to bind aflatoxin B1

“…The lyophilized strain was reactivated in MRS broth (Acumedia  , Lansing, MI, USA), composition shown in Table 1, at 37 °C for 24 hours and grown under these conditions until achieving high cell concentration (> 10 9 cells/ml). Estimation of bacterial cell concentration was determined by the turbidimetric method (Begot et al, 1996). A bacterial cell concentration curve was constructed using the absorbance measured at 600 nm (Spectrumlab 22PC -Shanghai Lengguang Technology Co. Ltd, Shanghai, China) and the logarithmic value of bacterial cell concentration obtained by dilution and pour plate counting (Wehr & Frank, 2004) after incubation in MRS agar (Acumedia  , Lansing, MI, USA) at 37 °C for 48 hours under anaerobic conditions.…”

“…Its sterilization (121 °C, 15 minutes), proteins were hydrolyzed with a commercial protease, and to prevent Maillard reaction, peptone and yeast crude extract were added subsequently under aseptic conditions. Another bacterial cell concentration curve was constructed correlating the bacterial growth in MMW and the absorbance measured spectrophotometrically at 600 nm (R 2 = 0.999; Begot et al, 1996). The assays were conducted in 1 L Erlenmeyer flasks at 37 °C for 24 hours.…”

Syntheses of Biologically Non-Toxic ZnS:Mn Nanocrystals by Surface Capping with O-(2-aminoethyl)polyethylene Glycol and O-(2-carboxyethyl)polyethylene Glycol Molecules

“…For these experiments, the optical density (OD) 600 method was employed, which measures the degree of turbidity of the nutrient broth by filling with growing bacteria. 31 The more turbid solution scatters more scanned light, so that the intensities of the passing light decreases with the bacterial growth. Usually, spectroscopic bacterial growth monitoring is performed at a working range of 550-600 nm, because the nutrient broth has a very low OD value at this wavelength region.…”