Abstract:High-frequency recombination was obtained with temperature-sensitive, conditionally lethal mutants of the arenavirus Pichinde. Pichinde virus is a member of the Tacaribe complex of arenaviruses (3, 19, 22). The virus was isolated in 1965 from pooled liver, spleen, and kidney tissues obtained from a rodent, Oryzomys albigularis, which was trapped in a forest in the Pichinde Valley of Colombia, South
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