Recombinant lignin peroxidase-catalyzed decolorization of melanin using in-situ generated H2O2 for application in whitening cosmetics

Sung, Ho Joon; Khan, Mohd Faheem; Kim, Yong Hwan

doi:10.1016/j.ijbiomac.2019.06.026

Cited by 45 publications

(30 citation statements)

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“…We found 88% decolonization of lignin at pH 3 which indicated that low pH favors direct contact of the enzyme with VA. Similar findings were also reported by Sung et al (2019), where they found that low pH must be maintained for optimal catalytic efficiency. The decolorization increases slowly within the lower end of the tested temperature (20-40 °C) and decreases rapidly as the incubation temperature continues to increase, suggesting denaturation may occur at higher temperatures.…”

Section: Discussionsupporting

confidence: 89%

“…In addition, P. chrysosporium produces acid-stable LiP which causes melanin decolorization. The possible reason for improved catalysis of melanin by LiP could be attributed to the role of supramolecular interactions that help maintain appropriate protein conformation at higher proton concentrations 1 .…”

Section: Discussionmentioning

confidence: 99%

“…LiP is easily inactivated in their excess concentration which induces the rupture of the heme structure or formation of the inactive compound III. It is reported that this inhibitory effect can be subdued by oxidation of VA to VA cation radical 1 . LiP oxidizes VA to VA cation radicals, which acts as a redox mediator to decolorize melanin.…”

Section: Discussionmentioning

confidence: 99%

“…Melanin is a group of complex, recalcitrant, and pervasive polycyclic bio-polymeric skin pigments produced by specialized skin cells called melanocytes 1,2 . Its primary function is to protect skin against harmful UV radiations of sunlight by forming supranuclear caps around the DNA of the skin cells 3 .…”

mentioning

confidence: 99%

“…Microbial extracellular enzymes such as laccase, manganese peroxidase and lignin peroxidase (LiP) have been previously tested for melanin decolorization and considered as a potential eco-friendly alternative to the toxic chemicals 1 . Among these, LiP was found very effective for the decolorization of melanin because of its high redox potential to oxidize veratryl alcohol (VA) than the other related enzymes.…”

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confidence: 99%

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Enzymatic decolorization of melanin by lignin peroxidase from Phanerochaete chrysosporium

Sadaqat

Khatoon

Malik

et al. 2020

Sci Rep

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Skin darkening results as a consequence of the accumulation of skin pigment melanin. To combat this, the amplitude of skin lightening agents are commercially available, most of which inhibit melanin synthesis. Decolorization of melanin is an alternative method of skin lightening. In this study, we show that lignin peroxidase (LiP), an extracellular enzyme purified from Phanerochaete chrysosporium NK-1 isolated from a forest soil can effectively degrade and decolorize melanin in vitro. Decolorization conditions including pH, temperature, incubation time, enzyme concentration, and mediator addition were investigated to optimize the reaction conditions. The results indicate that pH 3, 40 °C, 15 IU/ml, and 10 h incubation were the optimal conditions for the decolorization of the melanin. The use of the mediator, veratryl alcohol was also found effective to enhance the efficacy of the melanin decolonization, with up to 92% decolorization. The scanning electron microscopy results showed void spaces on the treated melanin granules as compared to the untreated sample, indicating the degradation of melanin. Changes in the fingerprint region of the melanin were observed. Between wavenumbers 1500–500 cm−1, for example, the presence of new peaks in the treated melanin at 1513, 1464, and 1139 cm−1 CH2, CH3 bend and C–O–C stretch represented structural changes. A new peak at 2144 cm−1 (alkynyl C≡C stretch) was also detected in the decolorized melanin. The cytotoxicity study has shown that the treated melanin and LiP have low cytotoxic effects; however, the mediator of veratryl alcohol could result in high mortality which suggests that its use should be meticulously tested in formulating health and skincare products. The findings of the study suggest that LiP produced by Phanerochaete chrysosporium has the potential to be used in the medical and cosmetic industries, particularly for the development of biobased cosmetic whitening agents.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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