Recombinant Human Erythropoietin Stimulates Angiogenesis and Healing of Ischemic Skin Wounds

Buemi, Michele; Galeano, Mariarosaria; Sturiale, Alessio; Ientile, Riccardo; Crisafulli, C.; Parisi, Antonio; Catania, MariaAntonietta; Calapai, Gioacchino; Impala, P; Aloisi, Carmela; Squadrito, Francesco; Altavilla, Domenica; Bitto, Alessandra; Tuccari, Giovanni; Frisina, N

doi:10.1097/01.shk.0000133591.47776.bd

Cited by 92 publications

(76 citation statements)

References 32 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition to preserving existing capillaries, EPO has also been shown to accelerate healing in the ischemic flap model by amplifying reparative angiogenesis (10)(11)(12). It is known that in organ injury, such as a rodent myocardial infarction model (31), EPO mobilizes endothelial progenitor cells (CD34 + /flk-1) that subsequently travel via the circulation and migrate into injury sites to drive neoangiogenesis, ultimately reducing tissue hypoxia.…”

Section: Discussionmentioning

confidence: 99%

“…Among the pleiotropic effects of EPO reported, a number of investigators have reported significant activity of EPO in promoting the healing of ischemic raised cutaneous skin flaps (10)(11)(12)(13)(14), ischemiareperfusion in random musculocutaneous flaps (15), incisional wounds (10,13), and in colonic anastomoses (16,17). Additionally, skin thermal burns also have been reported to respond in a beneficial way to EPO (18).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nonerythropoietic Tissue Protective Compounds Are Highly Effective Facilitators of Wound Healing

Erbayraktar

Yılmaz

et al. 2009

Mol Med

View full text Add to dashboard Cite

Erythropoietin (EPO) is a type I cytokine that utilizes different receptor isoforms either to maintain hematopoiesis or protect against injuries that arise from widely diverse etiologies. EPO also facilitates healing by reducing inflammation and mobilizing endothelial progenitor cells to participate in restorative neoangiogenesis, but it is unclear which EPO receptor isoform is responsible for healing and whether this receptor use varies according to the type of wound. In the present studies carried out in the rat, we have utilized receptor-selective derivatives of EPO to determine which receptor type operates in (i) a nonischemic wound (skin punch biopsy), (ii) a permanently ischemic wound (raised musculocutaneous flap), (iii) an intermittent ischemic reperfusion wound (pressure or decubitus ulcer), or (iv) wounds complicated by infection (cecal ligation and perforation). Using these models, we demonstrate that nonerythropoietic tissue protective compounds administered immediately following injury limit wound size and accelerate eschar closure independent of wound type. Moreover, in a model of peritonitis-induced adhesions, daily administration of the nonerythropoietic derivative carbamyl-EPO (10 μg/kg-bw) was associated with significantly lower serum TNFα concentration, illness scores, increased survival, as well as decreased adhesion formation. These results confirm that wound healing is mediated by the tissue protective receptor isoform and argue that nonerythropoietic tissue protective molecules constitute promising new therapeutics for treatment of a wide variety of surgical wounds.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nonerythropoietic Tissue Protective Compounds Are Highly Effective Facilitators of Wound Healing

Erbayraktar

Yılmaz

et al. 2009

Mol Med

View full text Add to dashboard Cite

show abstract

“…EPO is able to trigger healing by increasing angiogenesis, by the formation of granulation tissue, by the production of collagen as well as by re-epithelialisation [9,19,23]. Especially in complicated wounds like burns and under ischemic conditions, EPO seems to promote healing [8,20]. The prior aim was thus to verify whether there is a fundamental cellular response to EPO in vitro, which might serve to explain-at least in part-the healing improvement achieved by the application of EPO in vivo.…”

Section: Discussionmentioning

confidence: 97%

Erythropoietin ameliorates the reduced migration of human fibroblasts during in vitro hypoxia

et al. 2010

View full text Add to dashboard Cite

Erythropoietin promotes the formation of granulation tissue when administered to soft tissue wounds and it was shown to be most effective under tissue hypoxia. However, the action of erythropoietin on the cellular level is not well understood. In order to get a better insight into these processes, an in vitro wound healing assay was applied. Two main players of soft tissue healing-fibroblasts and microvascular endothelial cells-were used as mono- and co-cultures, subsequently inflicting in vitro wounds. Cell migration, proliferation, the differentiation of fibroblasts to myofibroblasts, and the release of vascular endothelial cell growth factor A and angiogenin were quantified in response to hypoxia and erythropoietin (5 IU/ml). Erythropoietin supplementation did neither affect proliferation nor migration of endothelial cells and fibroblasts under normoxia. Under hypoxia, the reduced fibroblast migration was ameliorated by erythropoietin. This effect coincided with an attenuated release of vascular endothelial growth factor A, whereas angiogenin release was unaffected by erythropoietin. The in vitro model applied in this study may represent an adequate approximation to certain aspects of the in vivo status of soft tissue regeneration and the results might serve to interpret the in vivo efficiency of erythropoietin at the cellular level: Erythropoietin has different impacts on the cells in normoxia and hypoxia. Its positive influence on fibroblast migration during hypoxia seems to support the strategies of applying erythropoietin in those chronic wounds, which exhibit fibroblastic dysfunction although good vascularisation is present.

show abstract

“…Supplying of oxygen and nutrients for collagen synthesis completed in the process of angiogenesis. 46 L. camara Linn. leaf extract ointment 5% more better than 10% for wound healing.…”

Section: Discussionmentioning

confidence: 99%

Efficcacy of Lantana camara Linn. leaf extracts ointment on dermal wound healing were infected with Staphylococcus epidermidis

Parwanto

2017

Int J Basic Clin Pharmacol

View full text Add to dashboard Cite

Background: Dermal infections by Staphylococcus epidermidis can cause problems for patients. Antibiotic resistance have been reported to treat the bacteria, therefore need to develop new drugs, among others sourced from herbs. For this purpose, study is needed to find an efficacy of Lantana camara Linn. leaf extract ointment on dermal wound healing were infected with S. epidermidis.Methods: Wistar rats divided into 4 groups randomly. Group I=dermal wounded, infected with S. epidermidis, untreated. Group II=dermal wounded, infected with S. epidermidis, treated with L. camara Linn. leaf extract ointment 5%. Group III=dermal wounded, infected with S. epidermidis, treated with L. camara Linn. leaf extract ointment 10%. Group IV=dermal wounded, infected with S. epidermidis, treated with sodium fusidate 2%. Bacterial colonies number observed on 6 days, while the quality of wound healing, measurement of DNA and protein levels on 6, 14 and 18 days. Mean ± SD between groups were analyzed by ANOVA.Results: Wound healing in group II qualitatively better than group I, III and IV. Bacterial colonies number in group II was lower than groups I, III and IV (p<0.05), whereas DNA and protein levels was higher in group II compare to groups I, III and IV (p<0.05). On 6, 14 and 18 days observations there are trend similarly ie DNA levels decreased, conversely protein levels increased.Conclusions: L. camara Linn. leaf extract ointment 5% more effective than 10% on dermal wound healing.

show abstract

Recombinant Human Erythropoietin Stimulates Angiogenesis and Healing of Ischemic Skin Wounds

Cited by 92 publications

References 32 publications

Nonerythropoietic Tissue Protective Compounds Are Highly Effective Facilitators of Wound Healing

Nonerythropoietic Tissue Protective Compounds Are Highly Effective Facilitators of Wound Healing

Erythropoietin ameliorates the reduced migration of human fibroblasts during in vitro hypoxia

Efficcacy of Lantana camara Linn. leaf extracts ointment on dermal wound healing were infected with Staphylococcus epidermidis

Contact Info

Product

Resources

About