Recombinant human collagen/chitosan-based soft hydrogels as biomaterials for soft tissue engineering

Yang, Yang; Ritchie, Alastair Campbell; Everitt, Nicola M.

doi:10.1016/j.msec.2020.111846

Cited by 52 publications

(29 citation statements)

References 49 publications

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“…Several kinds of hydrogel are used as wound dressings to maintain wound moisture and remove necrotic tissue. Among hydrogel materials, chitosan is biodegradable, biocompatible, and bio-adhesive, making it suitable for preparations aimed at healing HF burn wounds [17,18]. This study applied deacetylated chitosan-based hydrogels for the treatment HF.…”

Section: Discussionmentioning

confidence: 99%

Chitosan-based hydrogels to treat hydrofluoric acid burns and prevent infection

Huang

Yeh

et al. 2021

Drug Deliv. and Transl. Res.

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There is an urgent need for treatments for hydrofluoric acid (HF) burns and their derivative problems that prevent hydrogen ion dissociation and fluoride ion binding to tissues. This study evaluated the ability of chitosan-based hydrogels combined with a buffer solution containing either boric acid or Tris and calcium gluconate (CHS-BA-CG and CHS-Tris-CG) to repair HF burn wounds and prevent wound infections. We assessed calcium release rates and biocompatability and constructed a mouse HF burn model to assess the tissue repair effects of the hydrogels. Finally, we performed disc diffusion tests from burn tissue and quantified the bacterial counts to assess the anti-infection properties of the hydrogels. Calcium was gradually released in the CHS-BA-CG and CHS-Tris-CG groups (73% and 43%, respectively, after 48 h). The cell viabilities at 48 h after HF burn in these groups were significantly higher than those in the phosphate-buffered saline (PBS) and CG-treated groups. Histopathological evaluation showed a clear boundary between the epidermal and dermal layers in both CHS-BA-CG and CHS-Tris-CG-treated groups, indicating their effectiveness in tissue repair. In the disc diffusion test, CHS-BA-CG and CHS-Tris-CG exhibited larger inhibition zones against Acinetobacter baumannii than those for PBS and CG. The bacterial counts on HF burn wounds were significantly lower in the CHS-BA-CG and CHS-Tris-CG-treated groups than those in the PBS and CG-treated groups. The in vitro studies demonstrated the biocompatibility and antimicrobial effects of the CHS-BA-CG and CHS-Tris-CG hydrogels. Both gels also demonstrated tissue repair and anti-infection effects. Thus, chitosan-based hydrogels may be candidates for HF burn therapy.

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Section: Discussionmentioning

confidence: 99%

Chitosan-based hydrogels to treat hydrofluoric acid burns and prevent infection

Huang

Yeh

et al. 2021

Drug Deliv. and Transl. Res.

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“…Interestingly, both the cell proliferation rate and cell morphologies were found to depend on the hydrogel composition. Increasing the RHC fraction of the hydrogels gradually enhanced the cell spreading and proliferation rate [ 53 ].…”

Section: Preparation Methods Of Hydrogels With a Promising Treatment For Wound Healingmentioning

confidence: 99%

Hydrogel Preparation Methods and Biomaterials for Wound Dressing

Liang

et al. 2021

Life

134

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Wounds have become one of the causes of death worldwide. The metabolic disorder of the wound microenvironment can lead to a series of serious symptoms, especially chronic wounds that bring great pain to patients, and there is currently no effective and widely used wound dressing. Therefore, it is important to develop new multifunctional wound dressings. Hydrogel is an ideal dressing candidate because of its 3D structure, good permeability, excellent biocompatibility, and ability to provide a moist environment for wound repair, which overcomes the shortcomings of traditional dressings. This article first briefly introduces the skin wound healing process, then the preparation methods of hydrogel dressings and the characteristics of hydrogel wound dressings made of natural biomaterials and synthetic materials are introduced. Finally, the development prospects and challenges of hydrogel wound dressings are discussed.

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“…Recently the novel designs using two or three biomaterials like chitosan, hyaluronic acid, alginate, heparin, and collagen are being explored, as the single material cannot meet all the requirements ideal for biological applications. For example, biodegradable scaffolds of collagen and sodium alginate [77] and recombinant human collagen/chitosan hydrogels [78] are reported for soft tissue engineering applications, collagensilk composites have been used to regenerate the cartilage [79]. To overcome the traditional tissue or bone grafting method the chitosan-collagen composites are used for the development of synthetic graft to enhance the mechanical strength of the graft, as well as healing rate and bone adhesion rate [80].…”

Section: Novel Biomaterialsmentioning

confidence: 99%

A Guide to Collagen Sources, Applications and Current Advancements

Bhadra

Sakpal

Patil

et al. 2021

Systematic Bioscience and Engineering

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The increase in use of collagen products in almost every sector of utility has stipulated the rising demand for collagen. It is an essential fibrous glycoprotein, which is abundantly present in the extracellular matrix and connective tissues in many living organisms. Owing to its unique characteristics such as biocompatibility, bioavailability and weak antigenicity it has gained major interest in the food, pharmaceutical, cosmetic, biomedical and leather industries. The collagen-based composites possess an enhanced capacity to dissipate mechanical energy, strength and stiffness making it the most promising biomolecule for its multifaceted applications. Recently, synthetic biology platforms are getting wide attention for the production of non-native collagen alternatives to meet the rising demand for collagen worldwide. In the initial part, this review aims to explain different sources, structures, biosynthesis of collagen and its types followed by the emerging applications of collagen as a next-generation biomaterial in the later part. The article emphasises the synthesis of non-native collagen using novel expression systems like plant and algae in addition to bacteria and yeast with industrial consideration. In the conclusion section, challenges and opportunities of using synthetic biology tools are described.

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Recombinant human collagen/chitosan-based soft hydrogels as biomaterials for soft tissue engineering

Cited by 52 publications

References 49 publications

Chitosan-based hydrogels to treat hydrofluoric acid burns and prevent infection

Chitosan-based hydrogels to treat hydrofluoric acid burns and prevent infection

Hydrogel Preparation Methods and Biomaterials for Wound Dressing

A Guide to Collagen Sources, Applications and Current Advancements

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