Recombinant Fc-IL-18BPc Isoform Inhibits IL-18-Induced Cytokine Production

Hong, Kwang Won; Oh, Kwangjun; Lee, Siyoung; Hong, Jaewoo; Choi, Jida; Kwak, Areum; Kang, Dongjun; Kim, Eunsom; Jo, Seunghyun; Jhun, Hyunjhung; Kim, Soohyun

doi:10.1089/hyb.2011.0085

Cited by 3 publications

(3 citation statements)

References 40 publications

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“…In order to detect the isoforms of giant panda IL-18BP, RACE amplifications of 3′ ends was performed. Human IL-18BP gene encodes for at least four distinct isoforms (IL18BPa, b, c and d) derived from mRNA splice variants isolated from several cDNA libraries (Hong et al, 2012). IL-18BP sequences differed primarily in their carboxyl termini whereas the N-terminal was identical (Kim et al, 2000;Dinarello, 2000).…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

“…IL-18BP sequences differed primarily in their carboxyl termini whereas the N-terminal was identical (Kim et al, 2000;Dinarello, 2000). Different isoforms of the human IL18BP, murine IL-18BP and rhesus IL-18BP shared 100% identity in the 5′-untranslated regions (5′-UTR) (Hong et al, 2012;Dinarello, 2000;Yellayi et al, 2010). So in this study, only 3′ RACE was used to test the polymorphisms of AmIL-18BP in the 3′-UTR.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

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Cloning and characterization of giant panda (Ailuropoda melanoleuca) IL-18 binding protein

Yan

Deng²,

Niu³

et al. 2016

Research in Veterinary Science

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The giant panda (Ailuropoda melanoleuca) is an endangered species. Interleukin-18 (IL-18) plays an important role in the innate and adaptive immune responses by inducing IFN-γ. IL-18 has been implicated in the pathogenesis of various diseases. IL-18 binding protein (IL-18BP) is an intrinsic inhibitor of IL-18 that possesses higher affinity to IL-18. In this study, we cloned and characterized IL-18BP in giant panda (AmIL-18BP) from the spleen. The amino acid sequence of giant panda IL-18BP ORF shared about 65% identities with other species. To evaluate the effects of AmIL-18BP on the immune responses, we expressed the recombinant AmIL-18BP in Escherichia coli BL21 (DE3).The fusing protein PET-AmIL-18BP was purified by nickel affinity column chromatography. The biological function of purified PET-AmIL-18BP was determined on mice splenocyte by qRT-PCR. The results showed that AmIL-18BP was functional and could significantly reduce IFN-γ production in murine splenocytes. These results will facilitate the study of protecting giant panda on etiology and immunology.

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Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

Cloning and characterization of giant panda (Ailuropoda melanoleuca) IL-18 binding protein

Yan

Deng²,

Niu³

et al. 2016

Research in Veterinary Science

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“…In chronic HCV infection, the implication of IL-18 has been repeatedly proven in several studies, first as a marker of inflammation and hepatic injury and, second, as a predictor of antiviral treatment response [54,58,59,62,67,94,[106][107][108]. IL-18 activity is determined in part by the action of an intrinsic inhibitor, an IL-18 binding protein (IL18BP), which has the ability to bind to a receptor site, preventing the action of IL-18 [59,60,[68][69][70].…”

Section: Introductionmentioning

confidence: 99%

Análise de polimorfismos, expressão gênica e níveis séricos de IL-18, IL18BP e IFN-y na infecção crônica pelo HCV e resolução espontânea

Faria¹

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Immune mechanisms in polymyositis and dermatomyositis and potential targets for therapy

Venalis

Lundberg

2013

Rheumatology

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PM and DM are characterized clinically by weakness and low endurance of skeletal muscle. Other organs are frequently involved, suggesting that idiopathic inflammatory myopathies (IIMs) are systemic inflammatory diseases. Involvement of immune mechanisms in IIMs is supported by the presence of T cells, macrophages and dendritic cells in muscle tissue, by the presence of autoantibodies and by HLA-DR being a strong genetic risk factor. T cells may have direct and indirect toxic effects on muscle fibres, causing muscle fibre necrosis and muscle weakness, but the target of the immune reaction is not known. A newly identified T cell subset, CD28(null) T cells, may have cytotoxic effects in the CD4(+) and CD8(+) T cell phenotype. These cells are apoptosis resistant and may contribute to treatment resistance. Several myositis-specific autoantibodies have been identified, but they are all directed against ubiquitously expressed autoantigens and the specificity of the T cell reactivity is not known. These autoantibodies are associated with distinct clinical phenotypes and some with distinct molecular pathways; e.g. sera from patients with anti-Jo-1 autoantibodies may activate the type I IFN system and these sera also contain high levels of B cell activating factor compared with other IIM subsets. The characterization of patients into subgroups based on autoantibody profiles seems to be a promising way to learn more about the specificities of the immune reactions. Careful phenotyping of infiltrating immune cells in muscle tissue before and after specific therapies and relating the molecular findings to clinical outcome measures may be another way to improve knowledge on specific immune mechanism in IIMs. Such information will be important for the development of new therapies.

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Recombinant Fc-IL-18BPc Isoform Inhibits IL-18-Induced Cytokine Production

Cited by 3 publications

References 40 publications

Cloning and characterization of giant panda (Ailuropoda melanoleuca) IL-18 binding protein

Cloning and characterization of giant panda (Ailuropoda melanoleuca) IL-18 binding protein

Análise de polimorfismos, expressão gênica e níveis séricos de IL-18, IL18BP e IFN-y na infecção crônica pelo HCV e resolução espontânea

Immune mechanisms in polymyositis and dermatomyositis and potential targets for therapy

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