J. Neurosci. Res. volume 86, issue 13, P2963-2972 2008 DOI: 10.1002/jnr.21740 View full text
Hao-Hsin Yu, Fe-Lin Lin Wu, Shan-Erh Lin, Li-Jiuan Shen

Abstract: Modulation of nitric oxide (NO) production is considered a promising approach to therapy of diseases involving excessive inducible nitric oxide synthase (iNOS) expression, such as certain neuronal diseases. Recombinant arginine deiminase (rADI, EC3.5.3.6) catalyzes the conversion of L-arginine (L-arg), the sole substrate of NOS for NO production, to L-citrulline (L-cit) and ammonia. To understand the effect of the depletion of L-arg by rADI on NO concentration and neuroprotection, a direct coculture of neuron …

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