Reclassification of the genus Leuconostoc and proposals of Fructobacillus fructosus gen. nov., comb. nov., Fructobacillus durionis comb. nov., Fructobacillus ficulneus comb. nov. and Fructobacillus pseudoficulneus comb. nov.

Endo, Akihito; Okada, Sanae

doi:10.1099/ijs.0.65609-0

Cited by 144 publications

(120 citation statements)

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“…Recently, all of the rod-shaped species, i.e. Leuconostoc fructosum, Leuconostoc durionis, Leuconostoc ficulneum and Leuconostoc pseudoficulneum, were reclassified on the basis of phylogenetic and morphological differences into the genus Fructobacillus (Endo & Okada, 2008). The majority of members of both genera were isolated from sugar-rich substrates, e.g.…”

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Leuconostoc palmae sp. nov., a novel lactic acid bacterium isolated from palm wine

Ehrmann

Freiding

Vogel

2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A novel lactic acid bacterium, strain TMW 2.694 T , was isolated among other lactic acid bacteria from palm wine, an alcoholic beverage produced from the sap of various palm tree species. Strain TMW 2.694 T is a Gram-positive, facultatively anaerobic, catalase-negative, non-spore-forming coccus, occurring in long chains. Phylogenetic analysis based on 16S rRNA gene sequencing positioned strain TMW 2.694 T in a distinct line of descent within the genus Leuconostoc, with the closest neighbours being Leuconostoc lactis JCM 6123 T (98.7 % sequence similarity) and Leuconostoc citreum DSM 5577 T (98.8 % sequence similarity). Comparative sequencing of the additional phylogenetic markers dnaK and recA confirmed the 16S rRNA gene tree topology. Genomic DNA-DNA similarities of strain TMW 2.694 T to L. lactis DSM 20202 T and L. citreum DSM 5577 T were 45.1 and 17.7 %, respectively. The DNA G+C content is 36.4 mol%. Thus, we propose a novel species within the genus Leuconostoc, with the name Leuconostoc palmae sp. nov. and the type strain TMW 2.694 T (5DSM 21144 T 5LMG 24510 T ).

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Leuconostoc palmae sp. nov., a novel lactic acid bacterium isolated from palm wine

Ehrmann

Freiding

Vogel

2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…Pyruvate and oxygen are used as electron acceptors. At the time of writing, only five species with these characteristics have been described, Fructobacillus fructosus, Fructobacillus durionis, Fructobacillus ficulneus and Fructobacillus pseudoficulneus (Endo & Okada, 2008) and Lactobacillus kunkeei (Endo et al, 2009).During a study of fructophilic LAB, three strains were isolated from flowers in South Africa (Endo et al, 2009). The isolates shared several common characteristics with known fructophilic LAB, but also had a few different characteristics.…”

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“…Fructophilic lactic acid bacteria (LAB) are a group of LAB described recently (Endo & Okada, 2008). These organisms are known to have unique characteristics, i.e.…”

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confidence: 99%

“…These organisms are known to have unique characteristics, i.e. they grow well on D-fructose or D-glucose in the presence of external electron acceptors, but grow poorly on D-glucose without electron acceptors (Endo & Okada, 2008). Pyruvate and oxygen are used as electron acceptors.…”

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“…The 30 % FYP broth was used for selection of fructophilic LAB. Species of the genus Fructobacillus are known to resist high osmotic pressure (Endo & Okada, 2008) and F. ficulneus was originally isolated using broth containing 30 % glucose (Antunes et al, 2002). After visible growth was observed, cultures from 30 % FYP broth were streaked onto FYP agar containing (l 21 ) 5 g CaCO 3 and 12 g agar.…”

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Lactobacillus florum sp. nov., a fructophilic species isolated from flowers

Endo

Futagawa-Endo

Sakamoto

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Three strains of fructophilic lactic acid bacteria were isolated from flowers in South Africa. The isolates formed a subcluster in the Lactobacillus buchneri phylogenetic group, closely related to Lactobacillus fructivorans, Lactobacillus homohiochii, Lactobacillus lindneri and Lactobacillus sanfranciscensis according to phylogenetic analysis based on the 16S rRNA gene sequences. Levels of DNA-DNA relatedness indicated that the three strains belonged to the same taxon and formed a genetically distinct group, well separated from their phylogenetic relatives. The three strains produced acids from only two of the 49 carbohydrates tested, i.e. D-glucose and Dfructose. D-Fructose was more rapidly fermented than D-glucose. Good growth was recorded on D-fructose or D-glucose in the presence of external electron acceptors. However, delayed growth was recorded on D-glucose without electron acceptors. The novel strains produced lactic acid, ethanol and acetic acid from D-glucose at a ratio of 1 : 0.8 : 0.2. These characteristics were distinct from other species of the genus Lactobacillus. Based on the data provided, the three isolates represent a fructophilic and novel species of the genus Lactobacillus, for which the name Lactobacillus florum sp. nov. is proposed. The type strain is F9-1 T (5JCM 16035 T 5DSM 22689 T 5NRIC 0771 T ).Fructophilic lactic acid bacteria (LAB) are a group of LAB described recently (Endo & Okada, 2008). These organisms are known to have unique characteristics, i.e. they grow well on D-fructose or D-glucose in the presence of external electron acceptors, but grow poorly on D-glucose without electron acceptors (Endo & Okada, 2008). Pyruvate and oxygen are used as electron acceptors. At the time of writing, only five species with these characteristics have been described, Fructobacillus fructosus, Fructobacillus durionis, Fructobacillus ficulneus and Fructobacillus pseudoficulneus (Endo & Okada, 2008) and Lactobacillus kunkeei (Endo et al., 2009).During a study of fructophilic LAB, three strains were isolated from flowers in South Africa (Endo et al., 2009). The isolates shared several common characteristics with known fructophilic LAB, but also had a few different characteristics. They formed a subcluster within the Lactobacillus buchneri cluster on phylogenetic analysis based on their 16S rRNA gene sequences and were closely related to Lactobacillus lindneri. Levels of DNA2DNA similarity and biochemical characteristics showed clear separation of the isolates from L. lindneri. This paper describes the taxonomic study of the three novel isolates and their classification as a novel species of the genus Lactobacillus. The three novel strains, F9-1 T , F9-2 and F17, grew well in FYP broth, but poorly in GYP broth and were thus regarded as fructophilic LAB. They were preserved at 280 u C in nutrient broth containing 20 % (v/v) glycerol.The 16S rRNA gene sequences of the three isolates were determined according to a previously described method (Endo & Okada, 2005). The closest recognized relatives of t...

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