Recent trends in research on congenital toxoplasmosis

Petersen, Eskild; Pollak, Arnold; Reiter‐Owona, Ingrid

doi:10.1016/s0020-7519(00)00140-5

Cited by 67 publications

(41 citation statements)

References 65 publications

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“…Currently, T. gondii tachyzoites are produced by serial passages in the peritoneal cavity of mice or cotton rats [1,3,35,43]. Although convenient and reliable, this technique is ethically undesirable.…”

Section: Discussionmentioning

confidence: 99%

Use of a serum-free medium to produce in vitro Neospora caninum and Toxoplasma gondii tachyzoites on Vero cells

et al. 2002

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-Neospora caninum and Toxoplasma gondii are cyst-forming coccidian parasites of human and veterinary clinical relevance. In vitro cultivation of the protozoans using Vero cells is usually performed in order to produce antigenic materials. Quantitative and qualitative comparisons of Vero cells grown in RPMI medium supplemented either with foetal calf serum (FCS), horse serum (HS) or a specific serum-free additive (DefCell) were performed. A serum-free cell culture system used to propagate N. caninum (NC-1 isolate) and T. gondii tachyzoites (Rh stain) were compared with the other two cell culture systems. FCS supplemented media was found to be more effective than the others in promoting Vero cells and N. caninum tachyzoites. However, it was found unable to support adequate T. gondii tachyzoite proliferation. Vero cells, T. gondii and N. caninum tachyzoite production gave similar growth patterns with either HS or DefCell supplemented media. Defcell was considered as a good alternative to supplement culture medium.Neospora caninum / Toxoplasma gondii / cell culture / serum-free medium Résumé -Utilisation d'un milieu sans sérum pour la production in vitro de tachyzoites de Neospora caninum et Toxoplasma gondii sur cellules Vero. Neospora caninum et Toxoplasma gondii sont deux sporozoaires présentant un intérêt en médecine humaine et vétérinaire. La culture in vitro utilisant, entre autres, les cellules Vero comme support de la multiplication des deux parasites, est généralement employée en vue de l'obtention de tachyzoïtes. Au cours de cette étude, une évaluation quantitative et 159 Vet. Res. 33 (2002) 159-168

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Section: Discussionmentioning

confidence: 99%

Use of a serum-free medium to produce in vitro Neospora caninum and Toxoplasma gondii tachyzoites on Vero cells

et al. 2002

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“…Primary infection during pregnancy might also result in congenital transmission and disease in newborns (Petersen et al, 2001). Infection with T. gondii initiates after ingestion of oocysts that originate from definitive feline hosts or tissue cysts present in undercooked infected meat (Tenter et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Activation of NF-κB byToxoplasma gondiicorrelates with increased expression of antiapoptotic genes and localization of phosphorylated IκB to the parasitophorous vacuole membrane

Molestina

Payne

Coppens

et al. 2003

Journal of Cell Science

156

174

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“…In human, it is a dangerous disease in immunocompromised and immunosuppressed patients and congenitally infected children (Tenter et al, 2000;Montoya and Liesenfeld, 2004). Primary infection in pregnant seronegative women can cause neonatal death, abortion and severe congenital abnormalities, such as hydrocephalus, blindness, chorioretinitis and mental retardation (Petersen et al, 2001). On the other side, toxoplasmosis in animals has great economic importance worldwide because it can cause stillbirth, abortions and neonatal loss especially in sheep and goats (Buxton, 1998;Buxton et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Development of Improved Preventive and Treatment Strategies for Controlling of Toxoplasmosis in Saudi Arabia

Ismael¹,

Swelum²,

Nassan³

2016

American Journal of Infectious Diseases

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Toxoplasmosis causes severe public health problems and has great veterinary and economic importance worldwide. The present study aims mainly to develop an improved preventive and treatment program for controlling toxoplasmosis in sheep and goats. This study was performed on sheep and goats farm containing 357 animals (300 sheep and 57 goats). This farm had a large numbers of barren ewes, history of abortions (below 4 months), stillbirths, malformations and neonatal losses. Blood samples were collected to determine humeral antibody responses and prevalence of toxoplasmosis. Sections of normal and abnormal tissues were fixed using 10% buffered formalin for histopathological examination. Different and new drug regimens and strategies were used for controlling toxoplasmosis. Sixty-four out of 357 sheep and goats were clinically affected with 17.9% overall morbidity rate. These animals showed certain clinical symptoms of toxoplasmosis, such as abortions and stillbirth. The seroprevalence rate was 15.4%. The titers of ELISA were high before treatment in positive cases while after treatment the titers were decreased for certain level that continued to the end of study. The field treatment revealed that the affected sheep and goats treated with camel lactoferrin (cLf) and chemotherapy combination regimen significantly reduced the overall severity of toxoplasmosis with reduction in IgG titers better than those treated with chemotherapy alone (Pyrimethamine and sulfamethazine) or cLf alone. In conclusion, cLf and chemotherapy combination regimen was success in control of toxoplasmosis in small ruminants.

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Recent trends in research on congenital toxoplasmosis

Cited by 67 publications

References 65 publications

Use of a serum-free medium to produce in vitro Neospora caninum and Toxoplasma gondii tachyzoites on Vero cells

Use of a serum-free medium to produce in vitro Neospora caninum and Toxoplasma gondii tachyzoites on Vero cells

Activation of NF-κB byToxoplasma gondiicorrelates with increased expression of antiapoptotic genes and localization of phosphorylated IκB to the parasitophorous vacuole membrane

Development of Improved Preventive and Treatment Strategies for Controlling of Toxoplasmosis in Saudi Arabia

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