Recent trends in electrochemical microRNA biosensors for early detection of cancer

Keshavarz, Mahin; Behpour, Mohsen; Rafiee‐Pour, Hossain‐Ali

doi:10.1039/c5ra01726b

Cited by 45 publications

(27 citation statements)

References 87 publications

(79 reference statements)

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“…what induces specific upregulation and down regulation patterns), they can then be used as potential therapeutic targets as well as refined into improved diagnostic biomarker panels. 13 …”

Section: Introductionmentioning

confidence: 99%

Emerging Biosensing Approaches for microRNA Analysis

2015

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“…what induces specific upregulation and down regulation patterns), they can then be used as potential therapeutic targets as well as refined into improved diagnostic biomarker panels. 13 …”

Section: Introductionmentioning

confidence: 99%

Emerging Biosensing Approaches for microRNA Analysis

2015

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“…miRNA is considered as an ideal biomarker for cancer detection in early stages. Electrochemical biosensors have been used widely due to the fact that they can provide simple, rapid, and reliable detection [101].…”

Section: Role Of Nanoparticles In Biosenseor Technologymentioning

confidence: 99%

“…Other miRNAs associated with cancer MDR include miR-27a and miR-451. These miRNAs have been detected in resistant breast cancer and leukemia cells as well as in their MP cargo [101] with the former possibly playing a role in the upregulation of ABCB1 transcript in recipient breast cancer cells. miR-345 and miR-7 have also been previously shown to target ABCC1 in MDR breast cancer cells relative to parental cells [118].…”

Section: Mirnas Are Important Regulators Of Cancer Mdr and Metastaticmentioning

confidence: 99%

Therapeutic prospects of microRNAs in cancer treatment through nanotechnology

Awasthi¹,

Rathbone

Hansbro

et al. 2017

Drug Deliv. and Transl. Res.

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MicroRNAs (miRNAs) represent a new class of diagnostic and prognostic biomarker as well as new therapeutic targets in cancer therapy. miRNAs are gaining significant interest due to extensive advancements in knowledge since their discovery and, more recently, their translational application as therapeutic moieties and targets in the management of disease. miRNAs used in the treatment of cancer would position them as a new class of emerging therapeutic agents. Indeed, numerous candidate miRNAs have been identified as having therapeutic application in the treatment of cancer, but there is still much to learn about how to transform these into effective, patient-compliant, and targeted drug delivery systems. In this mini review, we discuss the utility and potential of nanotechnology in miRNA formulation and delivery with particular emphasis on cancer, including their role in conferring multidrug resistance and metastatic capacity. This review benefits both the formulation and biological scientists in understanding and exploring the new vistas of miRNA delivery using nanotechnology in the cancer clinically.

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“…Moreover, it has been demonstrated that the circulatory miRNAs are noticeably resistant to RNase digestion, non-physiologic pH values, and high temperature. Hence, these miRNAs have been considered as promising biomarker for early cancer detection [8–9]. …”

Section: Introductionmentioning

confidence: 99%

“…Although there have been advantages to each of these methods, none of them meets the high standards for clinical evaluation of miRNAs. Furthermore, almost all these techniques suffer from some known disadvantages including long analysis time, expensive reagents, high requirements to technical equipment, lack of adequate specificity, and poor reproducibility [9]. On the other hand, the technical challenges for the quantitative analysis of miRNA become more complex due to their small size, low abundance, and high sequence similarity.…”

Section: Introductionmentioning

confidence: 99%

A novel electrochemical nanobiosensor for the ultrasensitive and specific detection of femtomolar-level gastric cancer biomarker miRNA-106a

Daneshpour¹,

Omidfar²,

Ghanbarian³

2016

Beilstein J. Nanotechnol.

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Gastric cancer (GC) is the second leading cause of cancer-related deaths all over the world. miR-106a is a circulatory oncogenic microRNA (miRNA), which overexpresses in various malignancies, especially in GC. In this study, an ultrasensitive electrochemical nanobiosensor was developed for the detection of miR-106a using a double-specific probe methodology and a gold–magnetic nanocomposite as tracing tag. The successful modification of the electrode and hybridization with the target miRNA were confirmed by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) methods. Differential pulse voltammetry (DPV) was used for quantitative evaluation of miR-106a via recording the reduction peak current of gold nanoparticles. The electrochemical signal had a linear relationship with the concentration of the target miRNA ranging from 1 × 10−3 pM to 1 × 103 pM, and the detection limit was 3 × 10−4 pM. The proposed miRNA-nanobiosensor showed remarkable selectivity, high specificity, agreeable storage stability, and great performance in real sample investigation with no pretreatment or amplification. Consequently, our biosensing strategy offers such a promising application to be used for clinical early detection of GC and additionally the screen of any miRNA sequence.

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Recent trends in electrochemical microRNA biosensors for early detection of cancer

Cited by 45 publications

References 87 publications

Emerging Biosensing Approaches for microRNA Analysis

Emerging Biosensing Approaches for microRNA Analysis

Therapeutic prospects of microRNAs in cancer treatment through nanotechnology

A novel electrochemical nanobiosensor for the ultrasensitive and specific detection of femtomolar-level gastric cancer biomarker miRNA-106a

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