Recent progress in cartilage tissue engineering

Keeney, Michael; Lai, Janice H.; Yang, Fan

doi:10.1016/j.copbio.2011.04.003

Cited by 100 publications

(75 citation statements)

References 42 publications

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“…Kwon et al, 2013;Li et al, 2006;Pilarek et al, 2014;Wojasiński et al, 2014) as a synthetic polymeric biomaterial for chondrocyte implant development, whose properties, such as mechanical strength, degradation rate and dimensions can all be easily controlled. There is a distinct need to develop alternative techniques of mass transfer intensification within such 3D dense biomaterial-based constructs/implants to prevent the expansion of detrimental effects caused by progressive necrosis of the cells (Keeney et al, 2011;Pilarek et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation

Pilarek

Godlewska

Kuźmińska

et al. 2017

Chemical and Process Engineering

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One of the actual challenges in tissue engineering applications is to efficiently produce as high of number of cells as it is only possible, in the shortest time. In static cultures, the production of animal cell biomass in integrated forms (i.e. aggregates, inoculated scaffolds) is limited due to inefficient diffusion of culture medium components observed in such non-mixed culture systems, especially in the case of cell-inoculated fiber-based dense 3D scaffolds, inside which the intensification of mass transfer is particularly important. The applicability of a prototyped, small-scale, continuously waveinduced agitated system for intensification of anchorage-dependent CP5 chondrocytes proliferation outside and inside three-dimensional poly(lactic acid) (PLA) scaffolds has been discussed. Fibrous PLA-based constructs have been inoculated with CP5 cells and then maintained in two independent incubation systems: (i) non-agitated conditions and (ii) culture with wave-induced agitation. Significantly higher values of the volumetric glucose consumption rate have been noted for the system with the wave-induced agitation. The advantage of the presented wave-induced agitation culture system has been confirmed by lower activity of lactate dehydrogenase (LDH) released from the cells in the samples of culture medium harvested from the agitated cultures, in contrast to rather high values of LDH activity measured for static conditions. Results of the proceeded experiments and their analysis clearly exhibited the feasibility of the culture system supported with continuously wave-induced agitation for robust proliferation of the CP5 chondrocytes on PLA-based structures. Aside from the practicability of the prototyped system, we believe that it could also be applied as a standard method offering advantages for all types of the daily routine laboratory-scale animal cell cultures utilizing various fiber-based biomaterials, with the use of only regular laboratory devices.

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Section: Resultsmentioning

confidence: 99%

Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation

Pilarek

Godlewska

Kuźmińska

et al. 2017

Chemical and Process Engineering

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“…Such technique constituted the early cartilage tissue engineering, however, it is also limited by the source of cells as well as the morbidity left at the donor site. [23,24] The progress of regenerative medicine has provided more alternatives for cartilage tissue engineering. Mesenchymal stem cells isolated from many tissues, including bone marrow, adipose tissue, synovium, and umbilical cord, have all been found to have a chondrogenic potential and can be applied for cartilage tissue engineering.…”

Section: Cartilage Tissue Engineeringmentioning

confidence: 99%

Stem Cell Research: A New Era for Reconstructive Surgery

Li¹,

Yang²

2012

Selected Topics in Plastic Reconstructive Surgery

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“…These are the two major limitations to the cartilage regeneration. Within cartilage ECM, collagen is the most abundant protein component [17], and it has been used as a natural biomaterial for different tissueengineering applications [6,[18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%