Recent applications of microemulsion electrokinetic chromatography

Abstract

Compared to MEKC, the presence of a water-immiscible oil phase in the microemulsion droplets of microemulsion EKC (MEEKC) gives rise to some special properties, such as enhanced solubilization capacity and enlarged migration window, which could allow for the improved separation of various hydrophobic and hydrophilic compounds, with reduced sample pretreatment steps, unique selectivities and/or higher efficiencies. Typically, stable and optically clear oil-in-water microemulsions containing a surfactant (SDS), oil (octane or heptane), and cosurfactant (1-butanol) in phosphate buffer are employed as separation media in conventional MEEKC. However, in recent years, the applicability of reverse MEEKC (water-in-oil microemulsions) has also been demonstrated, such as for the enhanced separation of highly hydrophobic substances. Also, during the past few years, the development and application of MEEKC for the separation of chiral molecules has been expanded, based on the use of enantioselective microemulsions that contained a chiral surfactant or chiral alcohol. On the other hand, the application of MEEKC for the characterization of the lipophilicity of chemical substances remains an active and important area of research, such as the use of multiplex MEEKC for the high-throughput determination of partition coefficients (log P values) of pharmaceutical compounds. In this review, recent applications of MEEKC (covering the period from 2003 to 2005) are reported. Emphases are placed on the discussion of MEEKC in the separation of chiral molecules and highly hydrophobic substances, as well as in the determination of partition coefficients, followed by a survey of recent applications of MEEKC in the analysis of pharmaceuticals, cosmetics and health-care products, biological and environmental compounds, plant materials, and foods.

Review of aqueous chiral electrokinetic chromatography (EKC) with an emphasis on chiral microemulsion EKC

“…Three recent reviews on MEEKC have included chiral applications [55][56][57]. The first separation via chiral MEEKC was reported by Aiken and Huie et al [58] in 1993 and utilized a chiral oil, (2R, 3R)-di-n-butyl tartrate, in conjunction with an achiral surfactant (SDS) and achiral cosurfactant (1-butanol) to achieve a selectivity of 2.6 for the enantiomers of ephedrine.…”

Two‐chiral component microemulsion EKC – chiral surfactant and chiral oil. Part 2: Diethyl tartrate

“…Among the surfactant-based chiral selectors, microemulsions offer greater flexibility in adjusting an enantioseparation, both in terms of selectivity [39] (more PSP components to vary/optimize) and a somewhat larger and more variable elution range [40][41][42] (stronger dependency on ionic surfactant concentration with other variables held constant), allowing the elution range to be fine-tuned if necessary to achieve a better compromise between resolution (higher elution range) and analysis time (lower elution range). Other benefits of microemulsion EKC (MEEKC) are the improved solubility of hydrophobic compounds and a more flexible PSP [40,43].…”

Recent advances in the application of capillary electromigration methods for food analysis

“…In addition, some other published reviews were focused on discussing the different applications of a particular CE technique, including food analysis. This is the case for instance of CE coupled to mass spectrometry (MS) [15][16][17], electrochromatography [18], CE coupled to chemiluminiscence detection [19], microemulsion electrokinetic chromatography (MEEKC) [20], sample treatment prior to CE-MS [21].…”