Recent Advances in Pestivirus Research

Baculovirus expression of pestivirus non-structural proteins

“…The VN assay is probably measuring antibodies against the viral (structural) glycoproteins only. Of the many monoclonal antibodies recognizing pestivirus antigens, VN activity has been found only for those reactive with the envelope glycoproteins (Collett et al, 1989). The present ELISA involves detection of antibodies to viral non-structural proteins which, in cattle, are directed principally against the p80 (87K) antigen (Donis & Dubovi, 1987;Collett et al, 1988a).…”

“…The p80 protein is highly conserved among pestiviruses (Collett et al, 1989;Meyers et al, 1989;Moormann et al, 1990) and is an immunodominant antigen, convalescent bovine sera containing high levels of antibodies to p80 (Donis & Dubovi, 1987). Given the level of expression of this antigen and its antigenicity, we have in preliminary work investigated the utility of bacD2-133 antigens produced in Sf9 cells for the detection of anti-BVDV antibodies in bovine sera.…”

“…The pestivirus genome is a positive-sense ssRNA of about 12 to 13 kb and consists of a single large open reading frame (ORF) encoding approximately 4000 amino acids (Collett et al, 1989;Collett, 1992). However larger genomic sizes have recently been observed for some bovine viral diarrhoea virus (BVDV) isolates .…”

Congenital Pestivirus Infection in Sheep, Ewes, Heifers and Calves with Bovine Pestivirus Strains and Detection of Anigenic Differences between Them

“…At necropsy. 21 days after challenge, groups I. II and III the ewes of groups had antibody titer in the ELISA and ewes were seropositive in the SNT (FiJ:s. 5,6,7,8). Virus isolation In experiment I all fetal organs of group B.…”

Development and Application of an Enzyme-Linked Immunosorbent Assay for Detection of Bovine Viral Diarrhea Antibody Based on Erns Glycoprotein Expressed in a Baculovirus System

“…Members of this genus include classical swine fever virus (CSFV) and border disease virus (BDV). 11 BVDV is responsible for reproductive, enteric, and respiratory syndromes and causes considerable economic loss to cattle farms throughout the world. 8,26,27 The infection is sustained in the population by persistently infected (PI) animals 14,18 that are immunotolerant to the persisting virus strain due to initiation of infection early in their intrauterine development.…”