Recent advances in micromechanical characterization of polymer, biomaterial, and cell surfaces with atomic force microscopy

Chyasnavichyus, Marius; Young, Seth L.; Tsukruk, Vladimir V.

doi:10.7567/jjap.54.08la02

Cited by 43 publications

(33 citation statements)

References 129 publications

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“…This fact enables a high speed and simultaneous capture of force−distance curves and topographic images. 28 The maximum force (peak force) is controlled at each pixel to obtain force−distance curves which are then used as feedback signal. Figure 1 shows an example of the PF-QNM force−distance curve corresponding to the sample (Alg2.5/Chi) 10 .…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Quantitative Nanomechanical Properties of Multilayer Films Made of Polysaccharides through Spray Assisted Layer-by-Layer Assembly

et al. 2016

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Nanomechanical properties of alginate/chitosan (Alg/Chi) multilayer films, obtained through spray assisted layer-by-layer assembly, were studied by means of PeakForce quantitative nanomechanical mapping atomic force microscopy (PF-QNM AFM). Prepared at two different alginate concentrations (1.0 and 2.5 mg/mL) and a fixed chitosan concentration (1.0 mg/mL), Alg/Chi films have an exponential growth in thickness with a transition to a linear growth toward a plateau by increasing the number of deposited bilayers. Height, elastic modulus, deformation, and adhesion maps were simultaneously recorded depending on the number of deposited bilayers. The elastic modulus of Alg/Chi films was found to be related to the mechanism of growth in contrast to the adhesion and deformation. A comparison of the nanomechanical properties obtained for non-cross-linked and thermally cross-linked Alg/Chi films revealed an increase of the elastic modulus after cross-linking regardless alginate concentration. The incorporation of iron oxide nanoparticles (NPs), during the spray preparation of the films, gave rise to nanocomposite Alg/Chi films with increased elastic moduli with the number of incorporated NPs layers. Deformation maps of the films strongly suggested the presence of empty spaces associated with the method of preparation. Finally, adhesion measurements point out to a significant role of NPs on the increase of the adhesion values found for nanocomposite films.

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Section: ■ Experimental Sectionmentioning

confidence: 99%

Quantitative Nanomechanical Properties of Multilayer Films Made of Polysaccharides through Spray Assisted Layer-by-Layer Assembly

et al. 2016

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“…This has already been achieved in several ways. One straightforward possibility is to extract the time-dependent elastic modulus from a viscoelastic model and insert it in an arbitrary contact mechanics theory [26,27]. Another approach is based on the simulation of the tip-sample contact [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Transverse viscoelastic properties of pulp fibers investigated with an atomic force microscopy method

et al. 2019

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Changes in moisture content of single pulp fibers have an immense influence on the behavior of paper and paper products. Here, an atomic force microscopy (AFM)-based method is applied to investigate the viscoelastic properties of pulp fibers at varying relative humidity (RH) in the transverse direction. Pulp fibers have not only anisotropic properties, but also a very rough surface due to their hierarchical structure. For this reason, we have developed a specific load schedule for the AFM-based test method to overcome uncertainties and limitations due to surface roughness of the pulp fibers. The evaluation of the experimental data combines contact mechanics and viscoelastic models which consist of springs and dashpots in series or parallel describing elastic and viscous behavior. Here, it will be demonstrated that the so-called Generalized Maxwell (GM) model yields comparable results for single pulp fibers at five different RH values and in water. The moisture changes lead to a decrease in the elastic modulus but increase in the relaxation effects with increasing RH. All the determined parameters for the elastic and viscous behavior exhibit a gradual decrease with increasing RH from 10 to 75% RH. The elastic moduli decrease by a factor of 10 and the viscosities are decreasing by a factor of 10-20. In water, there is an even more pronounced decrease of the elastic moduli by a factor 100, and the viscosities decrease by at least three orders of magnitude compared to 10% RH. This indicates that the mechanical response of pulp fibers in water is significantly different than in humid air. This is also illustrated by the fact that a GM model of order two suffices to describe the material behavior in humid air but a GM model of order three is necessary to fit the material behavior in water. A possible interpretation is an additional relaxation effect of the pulp fiber wall in water.

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“…[33][34][35][36][37][38] In addition, there are several competing theoretical models to interpret the data. [40][41][42][43] Let's summarize the AFM studies devoted to measure the viscoelastic properties of cells. These contributions can be broadly classified into two main categories, force oscillation and time response methods.…”

Section: Introductionmentioning

confidence: 99%

Time-resolved nanomechanics of a single cell under the depolymerization of the cytoskeleton

2017

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Single cell stiffness measurements consider cells as passive and elastic materials which react instantaneously to an external force. This approximation is at odds with the complex structure of the cell which includes solid and liquid components. Here we develop a force microscopy method to measure the time and frequency dependencies of the elastic modulus, the viscosity coefficient, the loss modulus and the relaxation time of a single live cell. These parameters have different time and frequency dependencies. At low modulation frequencies (0.2-4 Hz), the elastic modulus remains unchanged; the loss modulus increases while the viscosity and the relaxation time decrease. We have followed the evolution of a fibroblast cell subjected to the depolymerization of its F-actin cytoskeleton. The elastic modulus, the loss modulus and the viscous coefficient decrease with the exposure time to the depolymerization drug while the relaxation time increases. The latter effect reflects that the changes in the elastic response happen at a higher rate than those affecting the viscous flow. The observed behavior is compatible with a cell mechanical response described by the poroelastic model.

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Recent advances in micromechanical characterization of polymer, biomaterial, and cell surfaces with atomic force microscopy

Cited by 43 publications

References 129 publications

Quantitative Nanomechanical Properties of Multilayer Films Made of Polysaccharides through Spray Assisted Layer-by-Layer Assembly

Quantitative Nanomechanical Properties of Multilayer Films Made of Polysaccharides through Spray Assisted Layer-by-Layer Assembly

Transverse viscoelastic properties of pulp fibers investigated with an atomic force microscopy method

Time-resolved nanomechanics of a single cell under the depolymerization of the cytoskeleton

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