Recent advances in low‐cost microfluidic platforms for diagnostic applications

Coltro, Wendell K. T.; Cheng, Chao‐Min; Carrilho, Emanuel; Jesus, Dosil Pereira de

doi:10.1002/elps.201400006

Cited by 129 publications

(58 citation statements)

References 141 publications

(169 reference statements)

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“…Other examples include the mChip, a device that tests whole blood for HIV using a combined microfluidic and protein-based immunoassay approach [58], and the Ativa Micro Lab (Ativa Medical, St. Paul, MN, USA), which was recently shown to execute a number of distinct blood tests with the use of a single device [59][60][61]. One upcoming technology is Ativa's portable Micro Lab, which has the potential to perform up to 25 distinct blood tests utilizing its various disposable test cards for each test.…”

Section: Current Commercial Poc Technologiesmentioning

confidence: 99%

“…Thus, the common limitations of commercially available POC devices are accuracy, variations of cost per test, and the inability to simultaneously monitor multiple analytes. All of these factors are important and often required to make definitive diagnoses [61,[64][65][66][67]. As a consequence, research and engineering efforts are still required to address these factors to improve POC technology.…”

Section: Current Commercial Poc Technologiesmentioning

confidence: 99%

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Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

et al. 2017

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Point-of-care (POC) device development is a growing field that aims to develop low-cost, rapid, sensitive in-vitro diagnostic testing platforms that are portable, selfcontained, and can be used anywhere -from modern clinics to remote and low resource areas. In this review, surface enhanced Raman spectroscopy (SERS) is discussed as a solution to facilitating the translation of bioanalytical sensing to the POC. The potential for SERS to meet the widely accepted "ASSURED" (Affordable, Sensitive, Specific, Userfriendly, Rapid, Equipment-free, and Deliverable) criterion provided by the World Health Organization is discussed based on recent advances in SERS in vitro assay development. As SERS provides attractive characteristics for multiplexed sensing at low concentration limits with a high degree of specificity, it holds great promise for enhancing current efforts in rapid diagnostic testing. In outlining the progression of SERS techniques over the past years combined with recent developments in smart nanomaterials, high-throughput microfluidics, and low-cost paper diagnostics, an extensive number of new possibilities show potential for translating SERS biosensors to the POC.

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Section: Current Commercial Poc Technologiesmentioning

confidence: 99%

Section: Current Commercial Poc Technologiesmentioning

confidence: 99%

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

et al. 2017

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“…To validate the capability of fabricated channels as microfluidic devices, a "Y" design was used for fluid flow and theoretically compared with the literature (Figures 3(a)-3(c)). 5,8,10,11,14,16,18,20,[37][38][39][40][41][42][43] Other geometries were also fabricated (Figures 3(d) and 3(e)) to show the versatility of the targeted asymmetric calendaring and hydrophobization (TACH) method. The microfluidic devices were manufactured using paper and tested with dyed water (food color) for visualization.…”

Section: B Channel Fabricationmentioning

confidence: 99%

Paper-based microfluidic devices by asymmetric calendaring

et al. 2017

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We report a simple, efficient, one-step, affordable method to produce open-channel paper-based microfluidic channels. One surface of a sheet of paper is selectively calendared, with concomitant hydrophobization, to create the microfluidic channel. Our method involves asymmetric mechanical modification of a paper surface using a rolling ball (ball-point pen) under a controlled amount of applied stress (r z ) to ascertain that only one side is modified. A lubricating solvent (hexane) aids in the selective deformation. The lubricant also serves as a carrier for a perfluoroalkyl trichlorosilane allowing the channel to be made hydrophobic as it is formed. For brevity and clarity, we abbreviated this method as TACH (Targeted Asymmetric Calendaring and Hydrophobization). We demonstrate that TACH can be used to reliably produce channels of variable widths (size of the ball) and depths (number of passes), without affecting the nonworking surface of the paper. Using tomography, we demonstrate that these channels can vary from 10s to 100s of microns in diameter. The created hydrophobic barrier extends around the channel through wicking to ensure no leakages. We demonstrate, through modeling and fabrication, that flow properties of the resulting channels are analogous to conventional devices and are tunable based on associated dimensionless numbers. Published by AIP Publishing. [http://dx

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“…10 In the current study we have used cyclic polyolefins (COPs) which belong to a class of polymers employed due to their compatibility with biological materials, low autofluorescence, long shelf life and suitability for surface modification amongst others. 11,12 Multilayer alignment fabrication methodologies are commonly used to develop functional microdevices 13,14 , as lamination of polymer layers offers greater versatility and low cost. Zeonor® was chosen for this study, in conjunction with PSA (pressure sensitive adhesive), as both are suitable materials for simple fabrication and lamination methodologies.…”

Section: Introductionmentioning

confidence: 99%