Recent advances in enzymatic biosensors for point‐of‐care detection of biomolecules

Abstract

Late state‐of‐the‐art analytical methodologies in chromatography, spectroscopy, and electroanalysis have been developed to meet the challenges of changing environmental and health issues. The modern trends in developing new protocols emphasize economic, portable, nano, or even smaller sample sizes and less time‐consuming processes. This has led to the development of technology‐based biosensors which meet most of the above requirements. The lab‐on‐chip technology exploiting enzyme‐based biosensors has made the analytical processes very efficient, accurate, affordable, and requiring nano‐scale sample sizes. In this review, an attempt is being made to review the literature based on state‐of‐the‐art technology of enzyme‐based biosensors for the detection of biomolecules.

Layer-By-Layer Films of Silsesquioxane and Nickel(II) Tetrasulphophthalocyanine as Glucose Oxidase Platform Immobilization: Amperometric Determination of Glucose in Kombucha Beverages

“…Enzyme immobilization on the electrode surface is one of the most important factors to be considered in the construction of amperometric biosensors [44][45][46][47]. Different immobilization methods of the GOx enzyme on the electrode surface were studied: the conven-…”

“…Enzyme immobilization on the electrode surface is one of the most important factors to be considered in the construction of amperometric biosensors [44][45][46][47]. Different immobilization methods of the GOx enzyme on the electrode surface were studied: the conventional dip and drop-coating methods and using glutaraldehyde as a cross-linking agent, which are based on electrostatic interaction and covalent bonding, respectively.…”

Integrated microfluidic devices for point-of-care detection of bio-analytes and disease

“…Researchers have explored various enzyme-based biosensor technologies for biomolecule detection, motivated by advancements in analytical methodologies and the need for efficient, accurate, and economically viable alternatives. 73 This means that multiple tests can be performed simultaneously, with each test providing reliable and accurate results. In addition, the LOC platform is designed to function as its full-scale counterpart, making it a highly efficient and cost-effective solution for various laboratory needs.…”

Research progress in the detection of common foodborne hazardous substances based on functional nucleic acids biosensors

“…However, traditional methods have complex sample processing processes, expensive instruments, and defects for fast and sensitive on‐site inspection requirements. For pathogenic bacteria detection in food, it is necessary to overcome the limitations of the testing environment and adopt low‐cost and highly sensitive testing technologies (Gupta et al, 2022).…”