Recent Advancements in Developments of Novel Fluorescent Probes: In Cellulo Recognitions of Alkaline Phosphatases

Nanjappa, Madhusudan Dasnur; Pandith, Anup; Sankaran, Svetlana; Dorairaj, Dorothy Priyanka; Reddy, Anusha Anjaneya; Ramesh, Hari Prasad Badubanahalli

doi:10.3390/sym14081634

Cited by 4 publications

(1 citation statement)

References 98 publications

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“…At the same time, fluorescence imaging has also attracted tremendous attention for biological application due to its salient merits of high sensitivity, real-time and non-invasive manner, and facile equipment [ 5 , 6 , 7 , 8 , 9 , 10 ]. Surgical resection of tumors can sometimes lead to unclean or excessive resection, resulting in a high possibility of tumor recurrence and seriously affecting the therapeutic effect; therefore, methods of differentiating cancerous tissues from normal tissues will help surgeons to operate more smoothly and accurately [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Self-Assembly of Small Organic Molecules into Luminophores for Cancer Theranostic Applications

Wang

Yang

et al. 2022

Biosensors

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Self-assembled biomaterials have been widely explored for real-time fluorescence imaging, imaging-guided surgery, and targeted therapy for tumors, etc. In particular, small molecule-based self-assembly has been established as a reliable strategy for cancer theranostics due to the merits of small-sized molecules, multiple functions, and ease of synthesis and modification. In this review, we first briefly introduce the supramolecular chemistry of small organic molecules in cancer theranostics. Then, we summarize and discuss advanced small molecule-based self-assembly for cancer theranostics based on three types, including peptides, amphiphilic molecules, and aggregation-induced emission luminogens. Finally, we conclude with a perspective on future developments of small molecule-based self-assembled biomaterials integrating diagnosis and therapy for biomedical applications. These applications highlight the opportunities arising from the rational design of small organic molecules with self-assembly properties for precision medicine.

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Section: Introductionmentioning

confidence: 99%

Self-Assembly of Small Organic Molecules into Luminophores for Cancer Theranostic Applications

Wang

Yang

et al. 2022

Biosensors

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Methods to Characterise Enzyme Kinetics with Biological and Medicinal Substrates: The Case of Alkaline Phosphatase

Harroun

Vallée‐Bélisle

2023

Chemistry Methods

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Alkaline phosphatase (AP) enzymes are of broad interest in fields ranging from biochemistry and medicine to biotechnology and nanotechnology. Characterising the catalytic activity of AP is typically realised by either employing non‐natural signal‐generating substrates that are detectable by absorbance and fluorescence spectroscopy or by quantifying the release of inorganic phosphate by the classic malachite green assay. The latter method is often required for studying “spectroscopically silent” biomolecular substrates, but it does not enable continuous monitoring of kinetics in real‐time. In recent years, newer techniques for studying AP function have been developed to circumvent this limitation, including fluorescent and colourimetric substrate‐specific assays based on supramolecular chemistry, organic probes and nanomaterials, as well as other assays based on isothermal titration calorimetry, direct detection with infrared spectroscopy and mass spectrometry, and monitoring conformational change by fluorescent nanoantennas. Here, we review these strategies and comment on their strengths and weaknesses in the context of AP.

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Perspective on Aggregation-induced Emission (AIE) Materials for Pathogen Detection

Guan

Zhou

Zhu

2023

CAC

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The rapid and accurate identification of pathogens plays a crucial role in clinical practice, which helps to prevent, control, and treat pathogenic infections at the initial stage. The current available technologies for pathogen detection appear to be inadequate in dealing with cases such as COVID-19. More importantly, the frequent emergence of drug-resistant bacteria is gradually rendering the existing therapeutic options ineffective. Efforts are urgently required to focus on the development of diagnostic systems for point-of-care (POC) detection and high-throughput pathogen identification. Since 2001, a new class of aggregation-induced emission luminogen (AIEgen) with good photostability, high sensitivity, and improved signal-to-noise ratio has emerged as powerful fluorescent tools for various biosensing and cell imaging. Based on the unique fluorescence of AIEgens that becomes stronger upon aggregation, naked-eye detection in turn-on mode has gained a speedy development. A timely overview can not only provide a summary of the advances and challenges of AIEgens in pathogen detection but also offers systematic ideas for future developments. There are also expectations for in-depth interdisciplinary research in the field of analytical chemistry and microbiology.

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Recent Advancements in Developments of Novel Fluorescent Probes: In Cellulo Recognitions of Alkaline Phosphatases

Cited by 4 publications

References 98 publications

Self-Assembly of Small Organic Molecules into Luminophores for Cancer Theranostic Applications

Self-Assembly of Small Organic Molecules into Luminophores for Cancer Theranostic Applications

Methods to Characterise Enzyme Kinetics with Biological and Medicinal Substrates: The Case of Alkaline Phosphatase

Perspective on Aggregation-induced Emission (AIE) Materials for Pathogen Detection

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