Recapitulation of previous genome-wide association studies with two distinct pathophysiological entities of gastric cancer in the Korean population

Abstract

Gastric cancer (GC) is the most common malignancy. The incidence rates remain remarkably high in East Asians. Although genome-wide association studies in the Han Chinese and Japanese populations have so far yielded susceptibility loci for GC, these findings need to be validated in an independent ethnic group. To identify the potential heterogeneity by histological classified subtypes (intestinal and diffuse), we examined the previously reported associations in the Korean population. PRKAA1 at 5p13.1 was found to be more strongly associated with intestinal type (odds ratio, OR=1.39, 95% CI (confidence interval) =1.22-1.58, P=3.77 × 10(-7)) than diffuse type. In addition, PSCA at 8q23.3 was significantly replicated in diffuse type (OR=1.49, 95% CI=1.32-1.67, P=2.43 × 10(-11)) but far less significant in intestinal type. In conclusion, these findings could bring additional insights into the etiologic heterogeneity in gastric carcinogenesis mechanisms.

Additive interactions between PRKAA1 polymorphisms and Helicobacter pylori CagA infection associated with gastric cancer risk in Koreans

“…A comparison based on histological type showed significant additive interactions only in intestinal‐type cancer; consistent with our results, Hwang et al. also indicated that the PRKAA1 SNP was more strongly associated with intestinal‐type gastric cancer than diffuse‐type gastric cancer . In addition, intestinal‐type gastric cancer is associated more with HP infection when compared to diffuse‐type gastric cancer , since stratified analysis for interaction between the PRKAA1 rs13361707 SNP and HP infection according to the anatomic location of cancer and histological type of cancer was considerably low.…”

“…Quality and quantity requirements for genotyping DNA samples were determined by identifying intact DNA bands after 2% agarose gel electrophoresis, and their concentrations were measured at >20 ng/ μ L with a degree of purity >1.7. Genotype analysis was performed for the five single‐nucleotide polymorphisms (SNPs; rs10074991, rs13361707, rs154268, rs3805486, and rs6882903) associated with PRKAA1 using a GoldenGate assay (Illumina, San Diego, CA, USA). The call rates for the examined samples and SNPs were 100%, with all five SNPs meeting the Hardy–Weinberg equilibrium in the control group.…”

Genome-wide association study of gastric adenocarcinoma in Asia: a comparison of associations between cardia and non-cardia tumours

“…Shi et al 9 reported a genome-wide significant association for this SNP in a scan of non-cardia cancer and also tested the association using DNA samples from 905 cardia cancer patients. Other reports relating this SNP to gastric cancer from Korea have also confirmed an association, 12 with one study reporting that the association did not differ by location in the stomach. 13 Overall, there appears to be consistent evidence for an association with gastric non-cardia cancer, but the results for cardia are mixed.…”

PSCA s2294008 C>T and rs2976392 G>A polymorphisms contribute to cancer susceptibility: evidence from published studies

“…Of them, two studies written in Chinese were excluded because of the overlap of study subjects [22, 32]. Moreover, one investigation was excluded for only focused on gastric survival not case-control study [51], three publications were excluded due to lack of genotyping data [52-54], one was not focused on cancer [55], and another two that did not pertain to either of these two polymorphism were also excluded [56, 57]. Eventually, 32 publications were included in the final meta-analysis.…”