Real-time PCR strategy for rapid discrimination among main lymnaeid species from Argentina

Duffy, Tomás; Kleiman, Florencia; Pietrokovsky, Silvia; Issia, Laura; Schijman, Alejandro G.; Wisnivesky‐Colli, Cristina

doi:10.1016/j.actatropica.2008.08.003

Cited by 14 publications

(10 citation statements)

References 25 publications

(28 reference statements)

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“…from Laguna Mucubaji (Venezuela) belong to the same evolutionary lineage and which is different of the one of L. ubaquensis from Laguna Ubaque (Colombia) and P. columella from Puerto Rico. The surprisingly high number of nucleotide differences in conserved positions of the 18S between our P. columella from Puerto Rico and the same species in Argentina [67] indicate that possibly another unknown lymnaeid species was involved in that study carried out in Argentina or, most probably, the Argentinean sequence was not sufficiently "clean". In one or another case, results showing a real-time, 18S-based PCR strategy to be useful for rapid discrimination among main lymnaeid species from Argentina [67] should be reassessed, even if the target used was the sequence fragment corresponding to the helix E10-1 of the variable area V2 highlighted by Bargues and Mas-Coma [46] and not the entire gene.…”

Section: Resultsmentioning

confidence: 99%

“…The surprisingly high number of nucleotide differences in conserved positions of the 18S between our P. columella from Puerto Rico and the same species in Argentina [67] indicate that possibly another unknown lymnaeid species was involved in that study carried out in Argentina or, most probably, the Argentinean sequence was not sufficiently "clean". In one or another case, results showing a real-time, 18S-based PCR strategy to be useful for rapid discrimination among main lymnaeid species from Argentina [67] should be reassessed, even if the target used was the sequence fragment corresponding to the helix E10-1 of the variable area V2 highlighted by Bargues and Mas-Coma [46] and not the entire gene. Moreover, this gene has recently proved to be useless for the discrimination of other lymnaeid species present in this country, as L. viatrix and L. neotropica which present identical 18S sequence [62].…”

Section: Resultsmentioning

confidence: 99%

“…- 18S rRNA gene: complete sequences of Lymnaea (Lymnaea) stagnalis [EMBL: Z73984], Lymnaea (Stagnicola) palustris [EMBL: Z73983], Omphiscola glabra [EMBL: Z73982], Galba truncatula [EMBL: Z73985] [46]; L. cubensis [EMBL: Z83831] [42,47]; L. viatrix and L. neotropica both species with the same sequence [EMBL: AM412222] [42]; Pseudosuccinea columella [GenBank: EU241866] [67]; Radix auricularia [EMBL: Z73980] and R. balthica [EMBL: Z73981] [46]. Other incomplete sequences available in the GenBank have not been used to avoid problems in comparative sequence analyses.…”

Section: Methodsmentioning

confidence: 99%

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DNA sequence characterisation and phylogeography of Lymnaea cousini and related species, vectors of fascioliasis in northern Andean countries, with description of L. meridensis n. sp. (Gastropoda: Lymnaeidae)

et al. 2011

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BackgroundLivestock fascioliasis is a problem throughout Ecuador, Colombia and Venezuela, mainly in Andean areas where the disease also appears to affect humans. Transmission patterns and epidemiological scenarios of liver fluke infection have shown to differ according to the lymnaeid vector snail species involved. These Andean countries present the vectors Lymnaea cousini, L. bogotensis and L. ubaquensis, unknown in the rest of Latin America. An exhaustive combined haplotype study of these species is performed by means of DNA sequencing of the nuclear ribosomal 18S RNA gene, ITS-2 and ITS-1, and mitochondrial DNA cox1 gene.ResultsThe conserved 5.8S rDNA sequence corroborated that no pseudogenes are involved in the numerous non-microsatellite/minisatellite-related indels appearing between the ITS-2 and ITS-1 sequences when comparing different L. cousini - L. bogotensis populations. Sequence analyses and phylogenetic reconstruction methods including other lymnaeid vector species show that (i) L. bogotensis is a synonym of L. cousini, (ii) L. ubaquensis is a synonym of Pseudosuccinea columella, and (iii) populations of L. cousini hitherto known from Venezuelan highlands indeed belong to a new species for which the name L. meridensis n. sp. is proposed. This new species is described and a complete phenotypic differentiation provided.ConclusionsITS-2, ITS-1 and cox1 prove to be good markers for specimen classification and haplotype characterisation of these morphologically similar lymnaeids in endemic areas. Analysis of the 18S gene and phylogenetic reconstructions indicate that L. cousini and L. meridensis n. sp. cluster in an evolutionary line different from the one of P. columella, despite their external resemblance. This suggests an evolutionary phenotypic convergence related to similar environments and which has given rise to frequent specimen misclassification. Body size and phylogenetic relationships of L. meridensis n. sp. with well-known vectors as Lymnaea cousini and P. columella, as well as with Galba/Fossaria species, suggest that the new species may participate in disease transmission to both animals and humans in altitude areas during the yearly window in which temperatures are higher than the F. hepatica minimum development threshold. The involvement of L. cousini and P. columella in the transmission and geographical/altitudinal distribution of fascioliasis in these Andean countries is analysed.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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DNA sequence characterisation and phylogeography of Lymnaea cousini and related species, vectors of fascioliasis in northern Andean countries, with description of L. meridensis n. sp. (Gastropoda: Lymnaeidae)

et al. 2011

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“…Sources: Bargues and Mas-Coma 1997 (Z73980-5); Bargues et al 1997 (Z83831); Bargues et al 2007 (AM412222); Bargues et al 2009 (FN182190); Bargues et al 2011a (FR772291); Bargues et al 2011b (FN598151-2); Bargues et al 2012 (JF909497); Correa et al 2011 (JN614326-68); Dayrat et al 2011 (HQ659965-6); Duffy et al 2009 (AF239912, AY057088-9, EU241865-6, EU728668); Jorgensen et al 2004 (AY577484); Klamath River at Collier Rest Area, California (HM230307*); Klussmann-Kolb et al 2008 (EF489345) (Note: their analyses excluded variable regions, so perhaps the region was excised from the published sequence rather than originally absent); Marquez, unpublished (Y09015-9); Owyhee River, Whistling Bird Rapids, Oregon (HM230306*); Stothard et al 2000 (AF192272-4); Vinarski et al 2011 (FR797815-29) Vonnemann et al 2005 (AY427525); Walker et al 2008 (EU152269, EU152270). Only the E10 region is considered above, so there may be differences in other parts of the sequence for ones that are grouped in the table.…”

Section: Resultsmentioning

confidence: 99%

Phylogenetic analysis of the Lancinae (Gastropoda, Lymnaeidae) with a description of the U.S. federally endangered Banbury Springs lanx

Campbell¹,

Clark²,

Lydeard³

2017

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We examined the patelliform snails of the subfamily Lancinae, endemic to northwestern North America, to test whether morphological variation correlated with genetic and anatomical differences. Molecular analyses using cox1, 16S, calmodulin intron, and 28S rDNA partial sequences and anatomical data supported recognition of four species in three genera. The relationships of lancines within Lymnaeidae are not yet well-resolved. The federally endangered Banbury Springs lanx is described as a new genus and species, Idaholanx fresti, confirming its distinctiveness and narrow endemicity.

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“…The first species came from a population living in San Pedro, Buenos Aires, Argentina (33° 40’ S, 59° 39’ W), while L. v. ventricosa originated from Paysandú, Uruguay (36° 00’ S, 57° 30’ W). As the systematics of South American lymnaeids is controversial and cannot be done with the use of single morphological criteria (Duffy et al ., 2009; Mera y Sierra et al ., 2009), the identification of these two species was performed using PCR-RFLP and sequencing of the ITS-1 segment of their nuclear rDNA (Sanabria et al ., 2012). The French population of G. truncatula was collected from a road ditch (45° 55’ 33’’ N, 2° 2’ 33’’ E) in the commune of Saint-Michel-de-Veisse, department of Creuse.…”

Section: Methodsmentioning

confidence: 99%

Paramphistomum daubneyi: characteristics of infection in three lymnaeid species

et al. 2012

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Experimental infections of two South American lymnaeids (Lymnaea neotropica and L. viatrix var. ventricosa) with Paramphistomum daubneyi were carried out to determine if these snail species could sustain larval development of this digenean and, if so, to specify their potential for cercarial production. A French population of Galba truncatula infected and raised according to the same protocol served as controls. In both experiments, prevalence of P. daubneyi infections in snails did not significantly differ from each other. In snail groups evaluated for cercarial shedding (first experiment), a significantly lower number of shed cercariae was noted for L. neotropica, while those from G. truncatula and L. v. ventricosa did not differ significantly from each other. Dissection of infected snails at day 65 post-exposure at 20 °C (second experiment) found significantly lower burdens of P. daubneyi rediae and cercariae in the bodies of L. neotropica than in those of G. truncatula and L. v. ventricosa. Compared to total cercarial production observed in dissected snails, the percentage of cercariae which exited from snails was 75.6% for G. truncatula, 21.6% for L. neotropica, and 91.4% for L. v. ventricosa. This last species seems to be a good candidate for metacercarial production of P. daubneyi.

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Real-time PCR strategy for rapid discrimination among main lymnaeid species from Argentina

Cited by 14 publications

References 25 publications

DNA sequence characterisation and phylogeography of Lymnaea cousini and related species, vectors of fascioliasis in northern Andean countries, with description of L. meridensis n. sp. (Gastropoda: Lymnaeidae)

DNA sequence characterisation and phylogeography of Lymnaea cousini and related species, vectors of fascioliasis in northern Andean countries, with description of L. meridensis n. sp. (Gastropoda: Lymnaeidae)

Phylogenetic analysis of the Lancinae (Gastropoda, Lymnaeidae) with a description of the U.S. federally endangered Banbury Springs lanx

Paramphistomum daubneyi: characteristics of infection in three lymnaeid species

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