Real-time detection of DNA during gel electrophoresis using a Zeeman refractive index detector

Johnston, Roger G.; Grace, W. Kevin; Lemanski, Cheryl L.; Mallawaaratchy, A. Rajika

doi:10.1016/0165-022x(94)90019-1

Cited by 5 publications

(6 citation statements)

References 21 publications

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“…Similarly, DNA sequencing can be implemented by detection of sub-nanometer level optical phase perturbations, as first studied by a Los Alamos Lab. group [13] and also suggested by N.A. Riza [14][15].…”

Section: Introductionmentioning

confidence: 77%

Ultrafast optical tomography systems using coherence agility

2004

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Described are three types of optical imaging systems based on source coherence agility. On axis ultrafast microsecond sub-surface imaging is achieved by use of a broadband low coherence optical source to implement fast Doppler time domain optical coherence tomography (OCT). This system is formed as a combination of a fast scan acousto-optically implemented variable optical delay line with a single acousto-optic (AO) Bragg cell optical heterodyne interferometer. A second imaging system is introduced with a no-moving parts probe design and fast microsecond speed optical spatial scanning along one dimension implemented using a fixed wavelength high coherence source with a single Bragg cell AO interferometer. The third design involves realization of a spectral domain OCT system implemented via the use of a tunable laser in the proposed single AO cell heterodyne interferometer.

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Section: Introductionmentioning

confidence: 77%

Ultrafast optical tomography systems using coherence agility

2004

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“…Applications include biological and biomedical research, polymer science, forensics, law enforcement, anthropology, biometrics, genetic counseling, botany, counter-tenorism, agriculture, animal husbandry, and biotechnology. In a paper published in the Journal of Biochemical & Biophysical Methods (JBBM), we reported RI measurements during DNA gel electrophoresis which had unprecedented resolution, stability, and linearity [9]. The measurements consistently showed a significant decrease in the refractive index (RI) as a DNA band appeared at a given location in the gel.…”

Section: Background and Research Objectivesmentioning

confidence: 99%

“…This anomalous decrease in RI hasn't been observed previously, apparently due to insufficient RI detector resolution and stability, insufficient temperature control, and problems with absolute quantification. In the JBBM paper, we speculated on 3 possible mechanisms that would cause a decrease in RI when a DNA band entered the probe region of the gel [9]. These possible mechanisms are:…”

Section: Background and Research Objectivesmentioning

confidence: 99%

“…Postulated mechanism (1) seemed the most likely, especially given the time-lag seen in the RI change when a DNA band entered the monitored region of the gel [9]. Mechanisms…”

Section: Background and Research Objectivesmentioning

confidence: 99%

“…] and refractive index (RI) measurements[5][6][7][8][9] have also been used for the real-time detection of DNA or proteins bands during electrophoresis.…”

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confidence: 99%

See 2 more Smart Citations

Thermal Detection of Dna and Proteins During Gel Electrophoresis

Johnston¹

2000

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Sensing and characterization technologies at Los Alamos National Laboratory

Eiden¹,

Hemberger²,

Johnston³

et al. 1996

Environ Monit Assess

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We describe four sensing and characterization technologies recently developed at Los Alamos National Laboratory; a select set of mass spectral and optical techniques is emphasized. This work describes new, or newly developed, technologies which can be used for on-site, at-line and laboratory analyses. These include two each of optical-and mass spectrometric-based systems. We describe first a field deployable mass spectrometer, based on an ion trap analyzer, and variants of that system. We then describe a hand-held, battery-operated optical spectrometer, usable in either absorption, or fluorescence excitation mode. A laser-based mass spectrometer is also described, which used a minimal tunable laser system, and a time-of-flight mass spectrometer. Finally, a Zeeman effect optical diffractomer is described.

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Real-time detection of DNA during gel electrophoresis using a Zeeman refractive index detector

Cited by 5 publications

References 21 publications

Ultrafast optical tomography systems using coherence agility

Ultrafast optical tomography systems using coherence agility

Thermal Detection of Dna and Proteins During Gel Electrophoresis

Sensing and characterization technologies at Los Alamos National Laboratory

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