Reagents for the Preparation of Chromophorically Labeled Polyethylene Glycol-Protein Conjugates

Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis

“…separation of proteins, it is not accepted as a routine But apparent overestimation and drawback might be solvent although some researchers use it as an given and the heterogeneity information of the additive. Our results indicate that large proportional PEGylated protein could not be obtained by these acetonitrile is efficient to enhance the resolution of methods [8][9][10]. Sodium dodecyl sulfate-polyPEGylated proteins, while the total analysis time is acrylamide gel electrophoresis (SDS-PAGE) [11], reduced compared to the reported methods.…”

Solubilization of Hydrophobic Peptides by Reversible Cysteine PEGylation

“…A more elegant if leu sensitive method of quantitation involves the use of chromophores. The derivatization of PEG with a chromophore allows quantitation of the derivative and of the amount of PEG attached to the target, in a nondestructive manner (Ladd & Snow, 1993). Nitrobenzoic acids contain a convenient carboxylic acid group for the attachent of PEG, while the chromophonc properties arr provided by the nitrobenzene ring.…”

“…Nitrobenzoic acids contain a convenient carboxylic acid group for the attachent of PEG, while the chromophonc properties arr provided by the nitrobenzene ring. MPEG has been esterified to lefluoronitrobenzoic aciâ, resulting in a chromophoric Lys-reactive PEG derivative (Ladd & Snow, 1993). However, no PEG derivatives have previously been synthesized that combine the advantages of Cys-reactivity with the chromophoric activity of the nitrobenzoic acids.…”

“…The procedure was broadly based on an analogous synthesis of an MPEGyl ester of 4-fluoro-3-nitrobenzoic acid (Ladd & Snow, 1993). Dithionitrobenzoic acid (1.0 rnmol, 0.40 g) and dicyclohexylcarbodiirnide (2.00 mmol, 2.00 mL of a 1.00 M solution in dichloromethane) were dissolved in 20 rnL of dichloromethane.…”

Preparation and characterization of poly(amidoamine) dendrimers functionalized with a rhenium carbonyl complex and PEG as new IR probes for carbonyl metallo immunoassay

“…doi:10.1016/j.jorganchem.2004.09.035 described the preparation of the 4th generation poly(amidoamine) (PAMAM-G4) dendrimer tethered with 45 CpFe(CO) 2 -succinimidato (Fp) units and four biotin entities [18]. More recently, we have reported the conjugation of such PAMAM-G4 bearing [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] Fp units with secondary antibodies, following a sitedirected multi-labeling procedure involving their glycosylated residues [19]. It is noteworthy that the use of dendrimers to achieve an amplification of the signal level in non-isotopic immunoassays is a relatively new concept [20,21].…”