Reactive oxygen species: potential cause for DNA fragmentation in human spermatozoa

Lopes, Stephanie; Jurisicova, Andrea; Sun, Jianguo; Casper, Robert F.

doi:10.1093/humrep/13.4.896

Cited by 465 publications

(185 citation statements)

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“…VEGF imunopositivity in testes from non-smokers and smokers. a 8-hydroxydeoxyguanosine in sperm DNA, supports such a theory [17,18]. Third, observed sperm DNA fragmentation is believed to be caused by apoptosis [19].…”

Section: Discussionmentioning

confidence: 52%

Caspase-3 and VEGF immunopositivity in seminiferous tubule germ cells in cases of obstructive and non-obstructive azoospermia in smokers versus non-smokers

Kılıç¹,

Lortlar²,

Bardakçı³

et al. 2008

J Assist Reprod Genet

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Purpose To investigate immunostaining pattern of caspase-3, an apoptosis marker, and vascular endothelial growth factor (VEGF), an hypoxia marker in testis biopsy specimens collected either from smoking or non-smoking patients with azoospermia. Methods Testis biopsy specimens were obtained from thirty seven non-smoker and thirty eight smoker patients. Using immunochemistry technique, caspase-3 and VEGF were evaluated in all intratubular spermatogenic and interstitial Leydig cells.Result(s) Caspase-3 expression was significantly increased in germ cells in maturation arrest specimens in smoker azoospermic patients. No statistically significant difference was present between smokers and non-smokers for caspase-3 expression in Sertoli cell. However, the VEGF immunopositive Leydig cells were statistically higher in smokers. There were no differences between groups in terms of germ cell immunopositivity. Conclusion Our results support the hypothesis that increased apoptosis contributes significantly to impaired spermatogenesis. We conjecture that germ cell apoptosis

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Section: Discussionmentioning

confidence: 52%

Caspase-3 and VEGF immunopositivity in seminiferous tubule germ cells in cases of obstructive and non-obstructive azoospermia in smokers versus non-smokers

Kılıç¹,

Lortlar²,

Bardakçı³

et al. 2008

J Assist Reprod Genet

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show abstract

“…Although the exact mechanisms that underlie the process of cryoinduced DNA damage are not fully understood, reactive oxygen species (ROS) formed during cryopreservation have been widely implicated in inducing DNA damage, both in fish (Li et al 2010) and in mammalian spermatozoa (Lopes et al 1998;Bennetts and Aitken 2005;Gosálvez et al 2014). An alternative explanation to the oxidative stress mechanism is the enhancement of pre-existing DNA damage, defects in DNA repair enzymes (Zribi et al 2010) or activation of caspase and apoptosis (Paasch et al 2004;Said et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Effects of freezing and activation on membrane quality and DNA damage in Xenopus tropicalis and Xenopus laevis spermatozoa

Morrow

Gosálvez

López‐Fernández

et al. 2017

Reprod. Fertil. Dev.

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Abstract. There is growing concern over the effect of sperm cryopreservation on DNA integrity and the subsequent development of offspring generated from this cryopreserved material. In the present study, membrane integrity and DNA stability of Xenopus laevis and Xenopus tropicalis spermatozoa were evaluated in response to cryopreservation with or without activation, a process that happens upon exposure to water to spermatozoa of some aquatic species. A dye exclusion assay revealed that sperm plasma membrane integrity in both species decreased after freezing, more so for X. laevis than X. tropicalis spermatozoa. The sperm chromatin dispersion (SCD) test showed that for both X. tropicalis and X. laevis, activated frozen spermatozoa produced the highest levels of DNA fragmentation compared with all fresh samples and frozen non-activated samples (P , 0.05). Understanding the nature of DNA and membrane damage that occurs in cryopreserved spermatozoa from Xenopus species represents the first step in exploiting these powerful model organisms to understand the developmental consequences of fertilising with cryopreservation-damaged spermatozoa.

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“…Probably the most important is the production of reactive oxygen species (ROS) which is excited, for example, by excessive stress, competitive sports, alcohol and drug abuse or nicotine. If produced in abundance, ROS can enter the cell nucleus, bind to the DNA and cause its fragmentation [15][16][17][18][19][20]. However, DNA fragmentation is also a feature of physiological processes like apoptosis and necrosis.…”

Section: Discussionmentioning

confidence: 99%

The correlation between male age, sperm quality and sperm DNA fragmentation in 320 men attending a fertility center

Winkle¹,

Rosenbusch

Gagsteiger³

et al. 2008

J Assist Reprod Genet

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Purpose To investigate the effects of male aging on sperm quality and sperm DNA fragmentation. Methods The ejaculates of 320 unselected men attending a fertility clinic and, as a control, 84 normozoospermic men without any history of ART were analyzed according to WHO guidelines. Sperm DNA fragmentation was measured by flow cytometry after staining with propidiumiodide. Results The patients were divided into four groups: <30 years, 30-35 years, 36-39 years and ≥40 years. Sperm motility decreased with increasing age whereas sperm concentration, morphology, and DNA fragmentation fluctuated throughout the four groups both among patients and among controls. However, we could not detect any significant correlation between male age and conventional semen parameters or sperm DNA fragmentation, respectively, neither in the patients' group nor among the controls. This also applies to a classification of patients and controls into only two age groups with a cut-off point at 35 years. Conclusions Our findings suggest that neither the routinely assessed semen parameters nor the amount of spermatozoa with fragmented DNA are affected by male age.

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Reactive oxygen species: potential cause for DNA fragmentation in human spermatozoa

Cited by 465 publications

References 0 publications

Caspase-3 and VEGF immunopositivity in seminiferous tubule germ cells in cases of obstructive and non-obstructive azoospermia in smokers versus non-smokers

Caspase-3 and VEGF immunopositivity in seminiferous tubule germ cells in cases of obstructive and non-obstructive azoospermia in smokers versus non-smokers

Effects of freezing and activation on membrane quality and DNA damage in Xenopus tropicalis and Xenopus laevis spermatozoa

The correlation between male age, sperm quality and sperm DNA fragmentation in 320 men attending a fertility center

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