Reactive Oxygen Species: Potential Cause for DNA Fragmentation in Human Spermatozoa

Lopes, Stephanie; Jurisicova, Andrea; Sun, Jianguo; Casper, Robert F.

doi:10.1016/s0022-5347(01)62472-8

Cited by 112 publications

(149 citation statements)

References 23 publications

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“…A positive relationship between poor sperm parameters and DNA damage in spermatozoa points to inherent spermatogenesis problems in specific patients [2]. The mechanisms of DNA damage in human spermatozoa include defective sperm chromatin packing, apoptosis and oxidative stress [3][4][5].…”

Section: Resultsmentioning

confidence: 99%

Comparative study of the effects of three semen preparation media on semen analysis, DNA damage and protamine deficiency, and the correlation between DNA integrity and sperm parameters

Chiamchanya¹,

Kaewnoonual²,

Visutakul³

et al. 2009

Asian J Androl

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Semen samples collected from 28 male partners of infertile couples were divided into three equal aliquots and prepared with three selected media, such as PureSperm  (Nidacon, Gothenburg, Sweden), Sil-Select Plus TM (Fertipro, Beernem, Belgium) and SpermGrad TM (Vitrolife, Gothenburg, Sweden). The differences in mean percentages of semen parameters were assessed by repeated measures analysis. Correlations of sperm DNA damage, as measured by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, and of protamine deficiency, as measured by chromomycin A3 (CMA3) staining with sperm parameters, were determined by Pearson's correlation. After preparation with all three media, sperm concentrations decreased (P < 0.05) while percentages of sperm with normal morphology increased (P < 0.05). Percentages of sperm motility, rapid motility and progressive motile concentration (PMC) increased (P < 0.05) for each of these parameters, PureSperm preparation gave the best results (P < 0.05). The percentage of DNA damage decreased in the PureSperm and Sil-Select Plus preparations (17.9% and 31.3%, respectively, P < 0.05) and increased in the SpermGrad preparation (56.3%, P < 0.05). Protamine deficiency also decreased in all three kinds of media, 59.3%, 47.7% and 40.3% for PureSperm, Sil-Select Plus and SpermGrad preparations, respectively (P < 0.05). The percentage of DNA-damaged sperm was negatively correlated with the percentages of sperm motility, rapid motility and PMC, but was positively correlated with static motility (P < 0.05). This comparative study and correlation analysis revealed that PureSperm preparation yielded sperm with the best motility and the lowest percentage of protamine deficiency. The Sil-Select Plus preparation yielded sperm with the lowest amount of DNA damage. The SpermGrad preparation had a high percentage of sperm with normal morphology, but also had the highest percentage of sperm with DNA damage. Sperm DNA damage was correlated with percentages of sperm motility, rapid motility, static motility and PMC. IntroductionIt is well known that semen quality is a significant factor for determining the success of pregnancy, apart from oocyte and endometrial quality. A semen prepa- Comparative study of the effects of three semen preparation media on semen analysis, DNA damage and protamine deficiency, and the correlation between DNA integrity and sperm parameters Comparative study of the effects of three semen preparation media Charoenchai Chiamchanya et al.

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Section: Resultsmentioning

confidence: 99%

Comparative study of the effects of three semen preparation media on semen analysis, DNA damage and protamine deficiency, and the correlation between DNA integrity and sperm parameters

Chiamchanya¹,

Kaewnoonual²,

Visutakul³

et al. 2009

Asian J Androl

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“…The duration of centrifugation was found to be more important than the force of centrifugation for inducing ROS formation by human spermatozoa (44). These data are important because exposing spermatozoa to high levels of ROS may cause DNA fragmentation, which may have adverse consequences if they are used for assisted reproductive techniques (ARTs) (45). This may be true particularly in light of a recent report by Zini and co-workers (46), who found that the improvement in sperm motility after Percoll processing was not associated with a similar improvement in sperm DNA integrity.…”

Section: Reactive Oxygen Species and Male Infertilitymentioning

confidence: 99%

“…Basal ROS ϭ levels of ROS in washed sperm suspensions after simple wash and resuspension in phosphate-buffered saline (containing sperm and leukocytes); pure sperm ROS ϭ levels of ROS in leukocyte-free sperm suspensions after complete removal of leukocytes using anti-CD 45 -coated paramagnetic beads. PMA-induced ROS ϭ levels of ROS in leukocyte-free sperm suspensions after stimulation of phorbol 12-myristate 13-acetate.…”

Section: Oxidative Stress and Sperm Suicide (Apoptosis)mentioning

confidence: 99%

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Role of reactive oxygen species in the pathophysiology of human reproduction

Agarwal

Saleh

Bedaiwy

2003

Fertility and Sterility

1,275

909

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Objective: To summarize the role of reactive oxygen species (ROS) in the pathophysiology of human reproduction. Design: Review of literature. Setting: Fertility research center and obstetrics and gynecology department in a tertiary care facility. Result(s): ROS plays an essential role in the pathogenesis of many reproductive processes. In male-factor infertility, oxidative stress attacks the fluidity of the sperm plasma membrane and the integrity of DNA in the sperm nucleus. Reactive oxygen species induced DNA damage may accelerate the process of germ cell apoptosis, leading to the decline in sperm counts associated with male infertility. ROS mediated female fertility disorders share many pathogenic similarities with the ones on the male side. These similarities include a potential role in the pathophysiology of endometriosis and unexplained infertility. High follicular fluid ROS levels are associated with negative IVF outcomes, particularly in smokers. Moreover, oxidative stress may be responsible in hydrosalpingeal fluid mediated embryotoxicity as well as poor in vitro embryonic development. Conclusion(s):High levels of ROS are detrimental to the fertility potential both in natural and assisted conception states. (Fertil Steril 2003;79:829 -43.

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“…ROS are needed for capacitation, the acrosome reaction and ultimately fertilisation. 4 However, excessive production and/or reduced clearance lead to oxidative stress within sperm, resulting in DNA damage, 5,6 reduced motility 7 and defective membrane integrity. 8,9 Antioxidants may help maintain the balance between ROS production and clearance and could thus improve sperm quality.…”

Section: Introductionmentioning

confidence: 99%

The role of antioxidant therapy in the treatment of male infertility: an overview

Lombardo¹,

Sansone²,

Romanelli³

et al. 2011

Asian J Androl

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In recent years, many studies have focused on the effect of oxidative stress, reactive oxygen species (ROS) and antioxidants on the male eproductive system. Under physiological conditions, sperm produces small amounts of ROS, which are needed for fertilisation, acrosome reaction and capacitation. However, if an increased production of ROS is not associated with a similar increase in scavenging systems, peroxidative damage of the sperm plasma membrane and loss of DNA integrity typically occur, which leads to cell death and reduced fertility. Furthermore, since there is no linear correlation between sperm quality and pregnancy rates, an improvement in semen parameters should not be the sole outcome considered in studies of antioxidant therapies. A definitive conclusion regarding the benefit of these therapies is difficult to obtain, as most of the previous studies lacked control groups, considered different antioxidants in different combinations and doses, or did not evaluate pregnancy rates in previously infertile couples. Even if beneficial effects were reported in a few cases of male infertility, more multicentre, double-blind studies performed with the same criteria are necessary for an increased understanding of the effects of various antioxidants on fertility.

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Reactive Oxygen Species: Potential Cause for DNA Fragmentation in Human Spermatozoa

Cited by 112 publications

References 23 publications

Comparative study of the effects of three semen preparation media on semen analysis, DNA damage and protamine deficiency, and the correlation between DNA integrity and sperm parameters

Comparative study of the effects of three semen preparation media on semen analysis, DNA damage and protamine deficiency, and the correlation between DNA integrity and sperm parameters

Role of reactive oxygen species in the pathophysiology of human reproduction

The role of antioxidant therapy in the treatment of male infertility: an overview

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