Reactive oxygen species and seed germination

Gomes, Marcelo Pedrosa; Garcia, Queila Souza

doi:10.2478/s11756-013-0161-y

Cited by 152 publications

(97 citation statements)

References 62 publications

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“…Reactive oxygen species (ROS) are considered toxic molecules formed during normal metabolic functions and induced when plants are exposed to environmental stimuli (Éaux and Toledano, 2007). However, they also act as signaling molecules in response to different stresses during germination (Gomes and Garcia, 2013;Tenhaken, 2015) or even germination under normal conditions, as occurs in Dalbergia nigra seeds (Matos et al, 2014). Reactions catalyzed by peroxidase also result in stimulation of sprouting, especially in the weakening of the cell wall, since they produce the hydroxyl radical (Richards et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Micropilar and embryonic events during hydration of Melanoxylon brauna Schott seeds

2015

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-Germination is a complex process that involves molecules properties that make up the cell walls, hydrolytic enzymes that break the bonds between the polymers and action of reactive oxygen substance. Melanoxylon brauna is a forest species of high economic value. In order to evaluate the physiological and biochemical changes that occur in the embryonic axis during germination, fresh matter, length, activities of the enzymes pectin methylesterase, polygalacturonase, superoxide dismutase, catalase, peroxidase and hydrogen peroxide levels were quantified in the embryonic axis. Furthermore, in the micropyle area the composition of carbohydrates and micropyle physical resistance were evaluated with and without drying. During soaking, if there are increases in fresh matter and length of the embryonic axis, there is the same trend of polygalacturonase and pectin methylesterase enzymes. The hydrogen peroxide content was reduced during the soaking, as well as the puncture force of the micropylar area. It is concluded that the seed coat and the cotyledons are responsible for 90% of the water soaked by the seeds. The events in the micropyle and embryonic axis occur independently in the first 16 hours. The weakening of the micropyle features an elastic step and a plastic one. Enzymes pectin methylesterase and polygalacturonase act in cellular expansion of the embryonic axis.Index terms: puncture force, hydrogen peroxide, cell wall, germination.Eventos micropilar e embrionário na hidratação de sementes de Melanoxylon brauna Schott RESUMO -A germinação é um processo complexo que envolve propriedades das moléculas que compõem a parede celular, enzimas hidrolíticas que quebram ligações entre polímeros da pectina e da hemicelulose e ação das substâncias reativas de oxigênio. Melanoxylon brauna é uma espécie florestal de alto valor econômico. Com o objetivo de avaliar alterações fisiológicas e bioquímicas no eixo embrionário durante a germinação, foram quantificados a massa fresca, o comprimento, as atividades das enzimas pectina metilesterase, poligalacturonase, superóxido dismutase, catalase, peroxidase e os teores de peróxido de hidrogênio. Foram avaliados na região micropilar a composição dos carboidratos e a resistência da micrópila seca e sem secagem. Durante a embebição, ocorrem aumentos na massa fresca e comprimento dos eixos embrionários, mesma tendência das enzimas poligalacturonase e pectina metilesterase, que participam da expansão celular. O conteúdo de peróxido de hidrogênio reduziu durante a embebição, assim como a força de ruptura da região micropilar. Conclui-se que o tegumento e os cotilédones são responsáveis por 90% da água embebida pelas sementes. Os eventos na micrópila e eixo ocorrem independentes nas primeiras 16 horas. O enfraquecimento da micrópila apresenta uma etapa elástica e outra plástica.As enzimas pectina metilesterase e a poligalacturonase atuam na expansão celular do eixo embrionário.Termos para indexação: força de ruptura, peróxido hidrogênio, parede celular, germinação.

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Section: Introductionmentioning

confidence: 99%

Micropilar and embryonic events during hydration of Melanoxylon brauna Schott seeds

2015

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“…In the last decade, it was suggested that reactive oxygen species ROS are also related to various aspects of plant physiology, such as dormancy and germination 1,2 . It is, thus, possible that ROS, along with plant hormones, constitutes an alternative form of regulation of plant physiology.…”

Section: Introductionmentioning

confidence: 99%

X-band Electron Paramagnetic Resonance Investigation of Stable Organic Radicals Present under Cold Stratification in ‘Fuji’ Apple Seeds

et al. 2017

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“…Superoxide dismutase, catalase, and peroxidase are enzymes that eliminate free radicals and peroxides, as well as malate dehydrogenase, acid phosphatase, and glutamate dehydrogenase, which are indicative of deterioration, since they are involved in cell metabolism (Taveira et al, 2012). Thus, information on the activity of certain enzymes can be used in studies on seed deterioration (Gomes;Garcia, 2013). Taveira et al (2012), studying the protein profile of coffee seeds dried under different methods, observed greater activity of the catalase enzyme in seeds of worse physiological performance.…”

Section: Resultsmentioning

confidence: 99%

“…Consequently, membrane function is changed, which provokes lipid peroxidation, degradation of nucleic acids, and inactivation of enzymes (Garg;Manchanda, 2009). Reduction in the activity of scavenger enzymes increases the sensitivity of seeds to oxidative stress (Gomes;Garcia, 2013). Superoxide dismutase, catalase, and peroxidase are enzymes that eliminate free radicals and peroxides, as well as malate dehydrogenase, acid phosphatase, and glutamate dehydrogenase, which are indicative of deterioration, since they are involved in cell metabolism (Taveira et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Tolerance of Coffea arabica L. seeds to sub zero temperatures

et al. 2017

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Preservation of the quality of coffee seeds is hindered by their intermediate behavior in storage. However, long-term storage at sub zero temperatures may be achieved by adjusting the water content of the seeds. The aim of this study was to evaluate the tolerance of coffee seeds to freezing, in relation to physiological and enzymatic modifications. Coffee seeds were dried in two manners, rapid and slow, to water contents of interest, 0.67, 0.43, 0.25, 0.18, 0.11, and 0.05 g H 2 O g -¹ dw (dry basis). After drying, the seeds were stored at a temperature of -20 ºC and of 86 ºC for 24 hours and for 12 months, and then compared to seeds in cold storage at 10 ºC. The seeds were evaluated through calculation of percentage of normal seedlings, percentage of seedlings with expanded cotyledonary leaves, dry matter of roots and of hypocotyls, and viability of embryos in the tetrazolium test. Expression of the enzymes superoxide dismutase, catalase, and peroxidase were evaluated by means of electrophoretic analysis. Only seeds dried more slowly to 0.18 g H 2 O g -1 dw present relative tolerance to storing at -20 °C for 12 months. Coffee seeds do not tolerate storage at a temperature of -86 ºC for 12 months. Water contents below 0.11g H 2 O g -¹ dw and above 0.43 g H 2 O g -¹ dw hurt the physiological quality of coffee seeds, regardless of the type of drying, temperature, and storage period. Coffee seed embryos are more tolerant to desiccation and to freezing compared to whole seeds, especially when the seeds are dried to 0.05 g H 2 O g -¹ dw. The catalase enzyme can be used as a biochemical marker to study tolerance to freezing in coffee seeds.Index terms: Drying; silica gel; saturated saline solutions; freezing; antioxidant enzymes. RESUMOA preservação da qualidade de sementes de café é dificultada pelo comportamento intermediário no armazenamento. Porém, a conservação a longo prazo em temperaturas subzero pode ser conseguido com o ajuste do teor de água das sementes. Objetivou-se neste trabalho avaliar a tolerância de sementes de café ao congelamento, com relação às modificações fisiológicas e enzimáticas. As sementes foram submetidas a dois tipos de secagem, rápida e lenta, até os teores de água de interesse, de 0,67, 0,43, 0,25, 0,18, 0,11, 0,05 g H 2 O g -¹ dw (base seca). Após secagem, as sementes foram armazenadas em temperatura de -20 e de -86 ºC, por 24 horas e por 12 meses, sendo comparadas às sementes armazenadas em câmara fria a 10 ºC. As sementes foram avaliadas pela porcentagem de plântulas normais, plântulas com folhas cotiledonares expandidas, matéria seca de raízes e de hipocótilos e viabilidade dos embriões no teste de tetrazólio. A expressão das enzimas superóxido dismutase, catalase e peroxidase foi avaliada por meio de análise eletroforética. Apenas as sementes secadas lentamente até 0,18 g H 2 O g -¹ dw apresentam relativa tolerância ao armazenamento a -20 °C por 12 meses. Sementes de café não toleram o armazenamento à temperatura de -86 ºC por 12 meses. Umidades abaixo de 0,11g H 2 O g -¹ dw ...

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Reactive oxygen species and seed germination

Cited by 152 publications

References 62 publications

Micropilar and embryonic events during hydration of Melanoxylon brauna Schott seeds

Micropilar and embryonic events during hydration of Melanoxylon brauna Schott seeds

X-band Electron Paramagnetic Resonance Investigation of Stable Organic Radicals Present under Cold Stratification in ‘Fuji’ Apple Seeds

Tolerance of Coffea arabica L. seeds to sub zero temperatures

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