Reactive oxygen species and anti-oxidant defences in swine follicular fluids

Abstract

A growing body of evidence indicates that the pro-oxidant/anti-oxidant balance inside the ovarian follicle plays an important role in folliculogenesis. Therefore, the aim of the present study was to assess the redox status of follicular fluids collected from different-sized swine follicles. We quantified the most important reactive oxygen species (ROS), namely superoxide anion (O(2)(-)), hydrogen peroxide and hydroperoxides (ROOH); in addition, we examined the activity of the detoxifying enzymes superoxide dismutase, catalase (CAT) and glutathione peroxidase and the total non-enzymatic antioxidant capacity as determined by the ferric-reducing anti-oxidant power assay. Our data demonstrate that oxidative stress does not affect follicle growth because O(2)(-) levels do not change during follicle development, whereas concentrations of H2O2 and ROOH are reduced (P < 0.05). Surprisingly, all non-enzymatic and enzymatic scavengers examined in the present study, except for CAT, demonstrated reduced activity during follicle development (P < 0.05). Taken together, these results suggest that other factors could be involved in ROS detoxification during follicle development.

Relationship Between Follicular Fluid and Serum Levels of Vitamin C and Oocyte Morphology and Embryo Quality in Patients Undergoing In Vitro Fertilization

“…1) arresting nuclear maturation in vitro allowing optimal capacitation of cytoplasm, 2) growing oocytes inside the follicle, and 3) identification of biomarkers of oocyte competence in granulosa and cumulus cells and metabolites in the FF (32). The study of Basini et al in 2016 was different with our study in type of sample but was similar to in rreactive oxygen and anti-oxidant defenses in FFs (33).…”

Reactive oxygen and nitrogen species during meiotic resumption from diplotene arrest in mammalian oocytes

“…The Cu-Zn SOD and Mn SOD are important primary intracellular ROS scavenger enzymes [Yu, 1994;Matos et al, 2009] and their activities have been reported in preantral, antral, and dominant follicles [Laloraya et al, 1989[Laloraya et al, , 1998Suzuki et al, 1999;Fujii et al, 2005;Matos et al, 2009]. The higher levels of ROS have been reported in the follicular fluids of Swine and Cow [Basini et al, 2008;Rizzo et al, 2009].…”

Oxygen tension affects histone remodeling of in vitro–produced embryos in a bovine model

“…Although O 2 concentration in the oviduct of mammals varies between 2% and 9% [11], several culture systems [12][13][14] find success culturing at atmospheric condition (20%). However, this supraphysiological concentration has been associated with increased concentrations of reactive oxygen species, which alters the culture environment, thus hindering embryo development [15,16]. However, some laboratories find increased development when embryos are cultured in 20% O 2 and FBS augments blastocyst rates [17].…”