Re-assembled casein micelles and casein nanoparticles as nano-vehicles for ω-3 polyunsaturated fatty acids

Zimet, Patricia; Rosenberg, Dina; Livney, Yoav D.

doi:10.1016/j.foodhyd.2010.11.025

Cited by 233 publications

(120 citation statements)

References 28 publications

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“…Finally, the last procedure and arguably the most popular, consists of a hardening step or cross-linking process of the newly formed casein nanoparticles. Amongst others, this stabilization of casein nanoparticles can be obtained by thermal treatment (Zimet, Rosenberg, & Livney, 2011) or enzymatic cross-linkage with genipin (Elzoghby, Helmy, Samy, & Elgindy, 2013) or transglutaminase (Huppertz & de Kruif, 2008). In this work we describe the preparation of casein nanoparticles under mild conditions in an aqueous environment using a basic amino acid (eg.…”

Section: Discussionmentioning

confidence: 99%

Casein nanoparticles as carriers for the oral delivery of folic acid

et al. 2015

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Alimentary proteins can be viewed as an adequate material for the preparation of nanoparticles and microparticles. They offer several advantages such as their digestibility, price and a good capability to interact with a wide variety of compounds and nutrients. The aim of this work was to prepare and characterize casein nanoparticles for the oral delivery of folic acid. These nanoparticles were prepared by a coacervation process, stabilized with either lysine or arginine and, finally, dried by spray-drying. For some batches, the effect of a supplementary treatment of nanoparticles (before drying) with hydrodynamic high pressure on the properties of the resulting carriers was also evaluated. The resulting nanoparticles displayed a mean size close to 150 nm and a folic acid content of around 25 mg per mg nanoparticle. From the in vitro release studies, it was observed that casein nanoparticles acted as gastro-resistant devices and, thus, folic acid was only released under simulated intestinal conditions. For the pharmacokinetic study, folic acid was orally administered to laboratory animals as a single dose of 1 mg/kg. Animals treated with folic acidloaded casein nanoparticles displayed significantly higher serum levels than those observed in animals receiving an aqueous solution of the vitamin. As a consequence the oral bioavailability of folic acid when administered in casein nanoparticles was calculated to be around 52%, a 50% higher than with the traditional aqueous solution. Unfortunately, the treatment of casein nanoparticles by hydrodynamic high pressure modified neither the release profile of the vitamin nor its oral bioavailability.

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Section: Discussionmentioning

confidence: 99%

Casein nanoparticles as carriers for the oral delivery of folic acid

et al. 2015

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“…CAS micelles effectively protected vitamin D 2 and docosahexaenoic acid against ultraviolet (UV) light-induced degradation and oxidation, respectively. 5,6 In another study, the complexation of curcumin with β-CAS micelles increased the solubility of curcumin at least 2500-fold with enhanced curcumin cytotoxicity to a human leukemia cell line. 7 Shapira et al 8,9 showed that β-CAS micelles could entrap and deliver hydrophobic chemotherapeutics such as mitoxantrone and paclitaxel, allowing them to be thermodynamically stable in aqueous solutions for oral delivery applications.…”

Section: Introductionmentioning

confidence: 98%

Novel ionically crosslinked casein nanoparticles for flutamide delivery: formulation, characterization, and in vivo pharmacokinetics

Elzoghby¹,

Helmy²,

Samy³

et al. 2013

IJN

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A novel particulate delivery matrix based on ionically crosslinked casein (CAS) nanoparticles was developed for controlled release of the poorly soluble anticancer drug flutamide (FLT). Nanoparticles were fabricated via oil-in-water emulsification then stabilized by ionic crosslinking of the positively charged CAS molecules below their isoelectric point, with the polyanionic crosslinker sodium tripolyphosphate. With the optimal preparation conditions, the drug loading and incorporation efficiency achieved were 8.73% and 64.55%, respectively. The nanoparticles exhibited a spherical shape with a size below 100 nm and a positive zeta potential (+7.54 to +17.3 mV). FLT was molecularly dispersed inside the nanoparticle protein matrix, as revealed by thermal analysis. The biodegradability of CAS nanoparticles in trypsin solution could be easily modulated by varying the sodium tripolyphosphate crosslinking density. A sustained release of FLT from CAS nanoparticles for up to 4 days was observed, depending on the crosslinking density. After intravenous administration of FLT-CAS nanoparticles into rats, CAS nanoparticles exhibited a longer circulation time and a markedly delayed blood clearance of FLT, with the half-life of FLT extended from 0.88 hours to 14.64 hours, compared with drug cosolvent. The results offer a promising method for tailoring biodegradable, drug-loaded CAS nanoparticles as controlled, long-circulating drug delivery systems of hydrophobic anticancer drugs in aqueous vehicles.

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“…BLG, the natural molecular nanocarrier for hydrophobic molecules, and its nanocomplexes with pectin can be used as vehicles for omega-3 polyunsaturated fatty acids for enrichment of clear acid drinks [341]. Zimet et al [342] prepared re-assembled casein micelles and casein NPs as nanovehicles for omega-3 polyunsaturated fatty acids such as docosahexaenoic acid (DHA) showing a remarkable protective effect against DHA oxidation and demonstrating good colloidal stability and bioactive conservation throughout shelf life at 4°C. Lipid NPs based on omega-3 fatty acids were also found to be effective carriers for lutein delivery and they showed excellent in vitro antioxidant activity by scavenging up to 98% oxygen free radicals.…”

Section: Nanotechnologies In Food Industrymentioning

confidence: 99%

Application Of Nanotechnology In Agriculture And Food Industry, Its Prospects And Risks

Jampílek

Kráľová

2015

Ecological Chemistry and Engineering S

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Nanoagrochemicals, such as nanopesticides, nanofertilizers or plant growth stimulating nanosystems, were primarily designed to increase solubility, enhance bioavailability, targeted delivery, controlled release and/or protection against degradation resulting in the reduced amount of applied active ingredients and finally in a decrease of dose-dependent toxicity/burden. This paper is a comprehensive up-to-date review related to the preparation and the biological activity of nanoformulations enabling gradual release of active ingredient into weeds and the body of pests and controlled release of nutrients to plants. The attention is also devoted to the decrease of direct environmental burden and economic benefits due to application of nanoformulations, where less amount of active ingredient is needed to achieve the same biological effect in comparison with bulk. The application of nanotechnology in the areas such as food packaging, food security, encapsulation of nutrients and development of new functional products is analysed. The use of nanoparticles in biosensors for detection of pathogens and contaminants as well as in DNA and gene delivery is discussed as well. Benefits and health risks of nanoagrochemicals are highlighted, and special attention is given to nanoecotoxicology and guidelines and regulatory documents related to the use of nanoformulations in agriculture and food industry.

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Re-assembled casein micelles and casein nanoparticles as nano-vehicles for ω-3 polyunsaturated fatty acids

Cited by 233 publications

References 28 publications

Casein nanoparticles as carriers for the oral delivery of folic acid

Casein nanoparticles as carriers for the oral delivery of folic acid

Novel ionically crosslinked casein nanoparticles for flutamide delivery: formulation, characterization, and in vivo pharmacokinetics

Application Of Nanotechnology In Agriculture And Food Industry, Its Prospects And Risks

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