RD26 mediates crosstalk between drought and brassinosteroid signalling pathways

Ye, Huaxun; Liu, Sanzhen; Tang, Buyun; Chen, Jiani; Xie, Zhouli; Nolan, Trevor M.; Jiang, Hao; Guo, Hongqing; Lin, Hung‐Ying; Li, Lei; Wang, Yanqun; Tong, Hongning; Zhang, Mingcai; Chu, Chengcai; Li, Zhaohu; Aluru, Maneesha; Aluru, Srinivas; Schnable, Patrick S.

doi:10.1038/ncomms14573

Cited by 209 publications

(223 citation statements)

References 71 publications

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“…However, transcript induction of other genes (such as SlRD29B, SlAOX1a, SlAOX2, SlSDR1C, SlDREB2A1 and SlDREB2A2) by SlTAF1 required salt, suggesting an involvement of a yet unknown salt activated factor(s) in the regulation of those genes by SlTAF1. RD26 was reported to regulate salt and drought stress Li et al, 2014;Ye et al, 2017). For example, JUB1 appears to enhance the tolerance towards drought and salt stress in part via an accumulation of proline in Arabidopsis, banana and tomato (A.…”

Section: Researchmentioning

confidence: 99%

Multifaceted regulatory function of tomato SlTAF1 in the response to salinity stress

et al. 2019

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Salinity stress limits plant growth and has a major impact on agricultural productivity. Here, we identify NAC transcription factor SlTAF1 as a regulator of salt tolerance in cultivated tomato (Solanum lycopersicum).While overexpression of SlTAF1 improves salinity tolerance compared with wild-type, lowering SlTAF1 expression causes stronger salinity-induced damage. Under salt stress, shoots of SlTAF1 knockdown plants accumulate more toxic Na + ions, while SlTAF1 overexpressors accumulate less ions, in accordance with an altered expression of the Na + transporter genes SlHKT1;1 and SlHKT1;2. Furthermore, stomatal conductance and pore area are increased in SlTAF1 knockdown plants during salinity stress, but decreased in SlTAF1 overexpressors.We identified stress-related transcription factor, abscisic acid metabolism and defence-related genes as potential direct targets of SlTAF1, correlating it with reactive oxygen species scavenging capacity and changes in hormonal response. Salinity-induced changes in tricarboxylic acid cycle intermediates and amino acids are more pronounced in SlTAF1 knockdown than wild-type plants, but less so in SlTAF1 overexpressors. The osmoprotectant proline accumulates more in SlTAF1 overexpressors than knockdown plants.In summary, SlTAF1 controls the tomato's response to salinity stress by combating both osmotic stress and ion toxicity, highlighting this gene as a promising candidate for the future breeding of stress-tolerant crops.

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Section: Researchmentioning

confidence: 99%

Multifaceted regulatory function of tomato SlTAF1 in the response to salinity stress

et al. 2019

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“…RD26, a member of the ATAF subfamily of TFs in A. thaliana (Jensen et al ., ), has previously been identified as a positive regulator of developmental and dark‐induced senescence (Li et al ., ) and jasmonic acid‐induced Chl degradation (Zhu et al ., ), while it is also involved in abiotic and biotic stress signaling (Fujita et al ., ; Tran et al ., ; Zheng et al ., ; Ye et al ., ). Here, we report RD26 as an important regulator of metabolic reprogramming during senescence by controlling the expression of genes across the entire spectrum of the cellular degradation hierarchy.…”

Section: Introductionmentioning

confidence: 97%

Transcription factor RD26 is a key regulator of metabolic reprogramming during dark‐induced senescence

et al. 2018

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SummaryLeaf senescence is a key process in plants that culminates in the degradation of cellular constituents and massive reprogramming of metabolism for the recovery of nutrients from aged leaves for their reuse in newly developing sinks.We used molecular-biological and metabolomics approaches to identify NAC transcription factor (TF) RD26 as an important regulator of metabolic reprogramming in Arabidopsis thaliana.RD26 directly activates CHLOROPLAST VESICULATION (CV), encoding a protein crucial for chloroplast protein degradation, concomitant with an enhanced protein loss in RD26 overexpressors during senescence, but a reduced decline of protein in rd26 knockout mutants. RD26 also directly activates LKR/SDH involved in lysine catabolism, and PES1 important for phytol degradation. Metabolic profiling revealed reduced c-aminobutyric acid (GABA) in RD26 overexpressors, accompanied by the induction of respective catabolic genes. Degradation of lysine, phytol and GABA is instrumental for maintaining mitochondrial respiration in carbon-limiting conditions during senescence.RD26 also supports the degradation of starch and the accumulation of mono-and disaccharides during senescence by directly enhancing the expression of AMY1, SFP1 and SWEET15 involved in carbohydrate metabolism and transport. Collectively, during senescence RD26 acts by controlling the expression of genes across the entire spectrum of the cellular degradation hierarchy.

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“…This approach enables various comparative physiological analyses (Salvi et al ). Interestingly, recent reports indicate that reduction of BR responses improves plant tolerance to drought (Feng et al , Northey et al , Ye et al ). Our recent results also indicated that under drought conditions the semi‐dwarf NILs exhibit delayed wilting when compared with a wild‐type cultivar (Gruszka et al ).…”

Section: Introductionmentioning

confidence: 99%

Non‐enzymatic antioxidant accumulations in BR‐deficient and BR‐insensitive barley mutants under control and drought conditions

Gruszka

Janeczko

Dziurka

et al. 2018

Physiologia Plantarum

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Drought is one of the most adverse stresses that affect plant growth and yield. Disturbances in metabolic activity resulting from drought cause overproduction of reactive oxygen species. It is postulated that brassinosteroids (BRs) regulate plant tolerance to the stress conditions, but the underlying mechanisms remain largely unknown. An involvement of endogenous BRs in regulation of the antioxidant homeostasis is not fully clarified either. Therefore, the aim of this study was to elucidate the role of endogenous BRs in regulation of non-enzymatic antioxidants in barley (Hordeum vulgare) under control and drought conditions. The plant material included the 'Bowman' cultivar and a group of semi-dwarf near-isogenic lines (NILs), representing mutants deficient in BR biosynthesis or signaling. In general, accumulations of 11 compounds representing various types of non-enzymatic antioxidants were analyzed under both conditions. The analyses of accumulations of reduced and oxidized forms of ascorbate indicated that the BR mutants contain significantly higher contents of dehydroascorbic acid under drought conditions when compared with the 'Bowman' cultivar. The analysis of glutathione accumulation indicated that under the control conditions the BR-insensitive NILs contained significantly lower concentrations of this antioxidant when compared with the rest of genotypes. Therefore, we postulate that BR sensitivity is required for normal accumulation of glutathione. A complete accumulation profile of various tocopherols indicated that functional BR biosynthesis and signaling are required for their normal accumulation under both conditions. Results of this study provided an insight into the role of endogenous BRs in regulation of the non-enzymatic antioxidant homeostasis.

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RD26 mediates crosstalk between drought and brassinosteroid signalling pathways

Cited by 209 publications

References 71 publications

Multifaceted regulatory function of tomato SlTAF1 in the response to salinity stress

Multifaceted regulatory function of tomato SlTAF1 in the response to salinity stress

Transcription factor RD26 is a key regulator of metabolic reprogramming during dark‐induced senescence

Non‐enzymatic antioxidant accumulations in BR‐deficient and BR‐insensitive barley mutants under control and drought conditions

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