Rational Design of Cytophilic and Cytophobic Polyelectrolyte Multilayer Thin Films

Mendelsohn, Jonas D.; Yang, Sung Yun; Hiller, Jeri'Ann; Hochbaum, Allon I.; Rubner, Michael F.

doi:10.1021/bm0256101

Cited by 436 publications

(575 citation statements)

References 55 publications

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“…A simple and versatile method for producing these architectures is the sequential build up of layers of functional materials by the layer-by-layer (LBL) technique [12,13]. This technique, initially applied to the production of polyelectrolyte thin films, has also been found to be suitable for the production of functionalized biomolecular architectures [14,15,16,17,18] and is therefore a relevant methodology for producing biological mimics to address radiation damage studies. However, it is fundamental to characterize the radiation degradation of the biological macromolecules in vacuum.…”

Section: A C C E P T E D Article In Pressmentioning

confidence: 99%

UV degradation of deoxyribonucleic acid

Gomes

Ribeiro

Shaw

et al. 2009

Polymer Degradation and Stability

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Section: A C C E P T E D Article In Pressmentioning

confidence: 99%

UV degradation of deoxyribonucleic acid

Gomes

Ribeiro

Shaw

et al. 2009

Polymer Degradation and Stability

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“…They 1) typically require adhesive proteins for cell attachment, [25] 2) are limited to specific parallel geometries defined by laminar flow patterns, [26] or 3) have been used predominantly with cell lines rather than primary cells due to the difficulties involved in attaching primary cells onto synthetic surfaces. [27][28][29][30][31] PEMs have been shown to be excellent candidates for biomaterial applications [31][32][33][34][35][36][37][38][39] and provide flexibility in building complex three-dimensional architectures. [40] The PEM surfaces also provide an ability to control the arrangement of multiple cell types with subcellular resolution.…”

Section: Introductionmentioning

confidence: 99%

Primary Neuron/Astrocyte Co‐Culture on Polyelectrolyte Multilayer Films: A Template for Studying Astrocyte‐Mediated Oxidative Stress in Neurons

Kidambi

Lee

Chan

2008

Adv Funct Materials

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We engineered patterned co-cultures of primary neurons and astrocytes on polyelectrolyte multilayer (PEM) films without the aid of adhesive proteins/ligands to study the oxidative stress mediated by astrocytes on neuronal cells. A number of studies have explored engineering coculture of neurons and astrocytes predominantly using cell lines rather than primary cells owing to the difficulties involved in attaching primary cells onto synthetic surfaces. To our knowledge this is the first demonstration of patterned co-culture of primary neurons and astrocytes for studying neuronal metabolism. In our study, we used synthetic polymers, namely poly(diallyldimethylammoniumchloride) (PDAC) and sulfonated poly(styrene) (SPS) as the polycation and polyanion, respectively, to build the multilayers. Primary neurons attached and spread preferentially on SPS surfaces, while primary astrocytes attached to both SPS and PDAC surfaces. SPS patterns were formed on PEM surfaces, either by microcontact printing SPS onto PDAC surfaces or vice-versa, to obtain patterns of primary neurons and patterned co-cultures of primary neurons and astrocytes. We further used the patterned co-culture system to study the neuronal response to elevated levels of free fatty acids as compared to the response in separated monoculture by measuring the level of reactive oxygen species (ROS; a widely accepted marker of oxidative stress). The elevation in the ROS levels was observed to occur earlier in the patterned co-culture system than in the separated monoculture system. The results suggest that this technique may provide a useful tool for engineering neuronal co-culture systems, that may more accurately capture neuronal function and metabolism, and thus could be used to obtain valuable

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“…18,19 Increasingly, polyelectrolyte multilayers (PEMs) are used as bioactive substrata for the study of cell adhesion or phenotype. 14,[20][21][22][23][24][25][26] PEMs are polyelectrolyte complexes fabricated via a layer-by-layer (LbL) assembly process with dilute solutions of positively and negatively charged polymers or by the LbL assembly of weakly interacting hydrogen bond acceptors/donors with polyelectrolyte polymers of complementary polarity. Because the physical properties and film thickness of weak (pHsensitive) PEMs can be controlled with high precision through assembly conditions such as solution pH, these materials find utility in a range of applications including but not limited to cytophilic substrata and cytophobic coatings.…”

Section: Introductionmentioning

confidence: 99%

Biochemical Functionalization of Polymeric Cell Substrata Can Alter Mechanical Compliance

et al. 2006

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Biochemical functionalization of surfaces is an increasingly utilized mechanism to promote or inhibit adhesion of cells. To promote mammalian cell adhesion, one common functionalization approach is surface conjugation of adhesion peptide sequences such as Arg-Gly-Asp (RGD), a ligand of transmembrane integrin molecules. It is generally assumed that such functionalization does not alter the local mechanical properties of the functionalized surface, as is important to interpretations of macromolecular mechanotransduction in cells. Here, we examine this assumption systematically, through nanomechanical measurement of the nominal elastic modulus of polymer multilayer films of nanoscale thickness, functionalized with RGD through different processing routes. We find that the method of biochemical functionalization can significantly alter mechanical compliance of polymeric substrata such as weak polyelectrolyte multilayers (PEMs), increasingly utilized materials for such studies. In particular, immersed adsorption of intermediate functionalization reagents significantly decreases compliance of the PEMs considered herein, whereas polymer-on-polymer stamping of these same reagents does not alter compliance of weak PEMs. This finding points to the potential unintended alteration of mechanical properties via surface functionalization and also suggests functionalization methods by which chemical and mechanical properties of cell substrata can be controlled independently.

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Rational Design of Cytophilic and Cytophobic Polyelectrolyte Multilayer Thin Films

Cited by 436 publications

References 55 publications

UV degradation of deoxyribonucleic acid

UV degradation of deoxyribonucleic acid

Primary Neuron/Astrocyte Co‐Culture on Polyelectrolyte Multilayer Films: A Template for Studying Astrocyte‐Mediated Oxidative Stress in Neurons

Biochemical Functionalization of Polymeric Cell Substrata Can Alter Mechanical Compliance

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