Rational design of affinity ligands for bioseparation

Matos, Manuel J.B.; Pina, Ana Sofia; Roque, Ana C. A.

doi:10.1016/j.chroma.2020.460871

Cited by 19 publications

(19 citation statements)

References 195 publications

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“…Improved engineered protein A variants with excellent properties, such as higher binding capacity and stability, have been used in commercial resins. With the advancement of rational design methods and the screening technology of combination libraries, novel and effective affinity ligands with desired properties have been successfully developed for application to antibody purification [ 77 ]. These ligands mainly contain alternative scaffold proteins, peptides, non-peptidyl ligands, and aptamers.…”

Section: Discussionmentioning

confidence: 99%

“…Generally, synthetic ligands are a type of low-molecular-weight compounds with properties of high chemical stability, robustness to sterilization, low cost, high versatility, and environmental friendliness [ 77 ]. Synthetic ligands mainly include three broad categories: peptidyl ligands, non-peptidyl ligands, and aptamers.…”

Section: Affinity Ligands and Corresponding Applications In Radiotherapymentioning

confidence: 99%

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Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy

Xue

Fan

2022

Biomolecules

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Antibodies have become an important class of biological products in cancer treatments such as radiotherapy. The growing therapeutic applications have driven a demand for high-purity antibodies. Affinity chromatography with a high affinity and specificity has always been utilized to separate antibodies from complex mixtures. Quality chromatographic components (matrices and affinity ligands) have either been found or generated to increase the purity and yield of antibodies. More importantly, some matrices (mainly particles) and affinity ligands (including design protocols) for antibody purification can act as radiosensitizers or carriers for therapeutic radionuclides (or for radiosensitizers) either directly or indirectly to improve the therapeutic efficiency of radiotherapy. This paper provides a brief overview on the matrices and ligands used in affinity chromatography that are involved in antibody purification and emphasizes their applications in radiotherapy to enrich potential approaches for improving the efficacy of radiotherapy.

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Section: Discussionmentioning

confidence: 99%

Section: Affinity Ligands and Corresponding Applications In Radiotherapymentioning

confidence: 99%

Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy

Xue

Fan

2022

Biomolecules

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“…Biomimetic molecular recognition has been applied in chromatographic systems by coupling ligands onto supports through capturing, separating, and determining template molecules. The use of ligands is based on highly specific and reversible interactions that create a selective and effective way [65]. Many different chromatographic systems have been described for determining the desired template molecules.…”

Section: Chromatographic Systemsmentioning

confidence: 99%

Advances in Biomimetic Systems for Molecular Recognition and Biosensing

et al. 2020

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Understanding the fundamentals of natural design, structure, and function has pushed the limits of current knowledge and has enabled us to transfer knowledge from the bench to the market as a product. In particular, biomimicry―one of the crucial strategies in this respect―has allowed researchers to tackle major challenges in the disciplines of engineering, biology, physics, materials science, and medicine. It has an enormous impact on these fields with pivotal applications, which are not limited to the applications of biocompatible tooth implants, programmable drug delivery systems, biocompatible tissue scaffolds, organ-on-a-chip systems, wearable platforms, molecularly imprinted polymers (MIPs), and smart biosensors. Among them, MIPs provide a versatile strategy to imitate the procedure of molecular recognition precisely, creating structural fingerprint replicas of molecules for biorecognition studies. Owing to their affordability, easy-to-fabricate/use features, stability, specificity, and multiplexing capabilities, host-guest recognition systems have largely benefitted from the MIP strategy. This review article is structured with four major points: (i) determining the requirement of biomimetic systems and denoting multiple examples in this manner; (ii) introducing the molecular imprinting method and reviewing recent literature to elaborate the power and impact of MIPs on a variety of scientific and industrial fields; (iii) exemplifying the MIP-integrated systems, i.e., chromatographic systems, lab-on-a-chip systems, and sensor systems; and (iv) closing remarks.

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“…One technology in particular is the combination of solid-phase extraction methods, using affinity-based ligands, with CE. Highly selective affinity ligands or adsorbents, such as antibodies, antibody fragments, lectins, aptamers, enzymes, metal-organic framework-based affinity sorbents, and other specially designed ligands are the cornerstone for the isolation and purification of many substances and cellular-subcellular entities found in complex matrices [ 25 , 47 , 54 , 55 , 56 , 57 , 58 , 75 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 ].…”

Section: Role Of Capillary Electrophoresis In Point-of-care Diagnomentioning

confidence: 99%

A Two-Dimensional Affinity Capture and Separation Mini-Platform for the Isolation, Enrichment, and Quantification of Biomarkers and Its Potential Use for Liquid Biopsy

Guzman¹,

Guzman

2020

Biomedicines

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Biomarker detection for disease diagnosis, prognosis, and therapeutic response is becoming increasingly reliable and accessible. Particularly, the identification of circulating cell-free chemical and biochemical substances, cellular and subcellular entities, and extracellular vesicles has demonstrated promising applications in understanding the physiologic and pathologic conditions of an individual. Traditionally, tissue biopsy has been the gold standard for the diagnosis of many diseases, especially cancer. More recently, liquid biopsy for biomarker detection has emerged as a non-invasive or minimally invasive and less costly method for diagnosis of both cancerous and non-cancerous diseases, while also offering information on the progression or improvement of disease. Unfortunately, the standardization of analytical methods to isolate and quantify circulating cells and extracellular vesicles, as well as their extracted biochemical constituents, is still cumbersome, time-consuming, and expensive. To address these limitations, we have developed a prototype of a portable, miniaturized instrument that uses immunoaffinity capillary electrophoresis (IACE) to isolate, concentrate, and analyze cell-free biomarkers and/or tissue or cell extracts present in biological fluids. Isolation and concentration of analytes is accomplished through binding to one or more biorecognition affinity ligands immobilized to a solid support, while separation and analysis are achieved by high-resolution capillary electrophoresis (CE) coupled to one or more detectors. When compared to other existing methods, the process of this affinity capture, enrichment, release, and separation of one or a panel of biomarkers can be carried out on-line with the advantages of being rapid, automated, and cost-effective. Additionally, it has the potential to demonstrate high analytical sensitivity, specificity, and selectivity. As the potential of liquid biopsy grows, so too does the demand for technical advances. In this review, we therefore discuss applications and limitations of liquid biopsy and hope to introduce the idea that our affinity capture-separation device could be used as a form of point-of-care (POC) diagnostic technology to isolate, concentrate, and analyze circulating cells, extracellular vesicles, and viruses.

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Rational design of affinity ligands for bioseparation

Cited by 19 publications

References 195 publications

Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy

Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy

Advances in Biomimetic Systems for Molecular Recognition and Biosensing

A Two-Dimensional Affinity Capture and Separation Mini-Platform for the Isolation, Enrichment, and Quantification of Biomarkers and Its Potential Use for Liquid Biopsy

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