Rational design of a self-assembled surfactant film in nanopipettes: combined fluorescence and electrochemical sensing

Liu, Qi; Ding, Shushu; Shi, Guoyue; Zhu, Anwei

doi:10.1039/d1cc06667f

Cited by 5 publications

(6 citation statements)

References 29 publications

(30 reference statements)

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“…[35] As an illustration, Zhu et al have presented an innovative approach to nanopipette functionalization. [36] They have utilized surfactant architectures to construct a nanopipette sensor on alkyl chain-modified nanopipettes. In their initial report, they described a label-free method for creating a nanopipette dual-signal sensing platform.…”

Section: Non-covalent Self-assemblymentioning

confidence: 99%

Solid‐State Glass Nanopipettes: Functionalization and Applications

Wang,

Tang,

Qiu

et al. 2024

Chemistry A European J

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Solid‐state glass nanopipettes provide a promising confined space that offers several advantages such as controllable size, simple preparation, low cost, good mechanical stability, and good thermal stability. These advantages make them an ideal choice for various applications such as biosensors, DNA sequencing, and drug delivery. In this review, we first delve into the functionalized nanopipettes for sensing various analytes and the methods used to develop detection means with them. Next, we provide an in‐depth overview of the advanced functionalization methodologies of nanopipettes based on diversified chemical kinetics. After that, we present the latest state‐of‐the‐art achievements and potential applications in detecting a wide range of targets, including ions, molecules, biological macromolecules, and single cells. We examine the various challenges that arise when working with these targets, as well as the innovative solutions developed to overcome them. The final section offers an in‐depth overview of the current development status, newest trends, and application prospects of sensors. Overall, this review provides a comprehensive and detailed analysis of the current state‐of‐the‐art functionalized nanopipette perception sensing and development of detection means and offers valuable insights into the prospects for this exciting field.

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Section: Non-covalent Self-assemblymentioning

confidence: 99%

Solid‐State Glass Nanopipettes: Functionalization and Applications

Wang,

Tang,

Qiu

et al. 2024

Chemistry A European J

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“…In addition to the high sensitivity to subtle changes in ionic flow, 7−11 nanopore analysis combined with fluorescence has been reported. 12,13 Typically, multiple dyes (e.g., quantum dots, chemical fluorophores, or fluorescent silica nanoparticles) were coated on the outer surface of pipet tips. 14−17 However, these materials often suffer from rapid photobleaching.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Up to now, single glass nanopores have been proven as a powerful technique for biosensing. In addition to the high sensitivity to subtle changes in ionic flow, − nanopore analysis combined with fluorescence has been reported. , Typically, multiple dyes (e.g., quantum dots, chemical fluorophores, or fluorescent silica nanoparticles) were coated on the outer surface of pipet tips. − However, these materials often suffer from rapid photobleaching. Recently, micropipettes made of terbium(III) (Tb 3+ )-doped borosilicate glass materials have been developed and were applied for the optical targeting of cells .…”

Section: Introductionmentioning

confidence: 99%

Rare Earth-Carbon Dots Nanocomposite-Modified Glass Nanopipettes: Electro-Optical Detection of Bacterial ppGpp

Ding,

Liu,

Zhu

et al. 2024

Anal. Chem.

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As an important alarmone nucleotide, guanosine 3′-diphosphate-5′-diphosphate (ppGpp) can regulate the survival of bacteria under strict environmental conditions. Direct detection of ppGpp in bacteria with high sensitivity and selectivity is crucial for elucidating the role of ppGpp in bacterial stringent response. Herein, the terbium−carbon dots nanocomposite (CDs-Tb) modified glass nanopipet was developed for the recognition of ppGpp. The CDs-Tb in glass nanopipette preserved their fluorescence properties as well as the coordination capacity of Tb 3+ toward ppGpp. The addition of ppGpp not only led to the fluorescence response of CDs-Tb but also triggered variations of surface charge inside the glass nanopipet, resulting in the ionic current response. Compared with nucleotides with similar structures, this method displayed good selectivity toward ppGpp. Moreover, the dual signals (fluorescence and ionic current) offered a built-in correction for potential interference. Apart from the high selectivity, the proposed method can determine the concentration of ppGpp from 10 −13 to 10 −7 M. Taking advantage of the significant analytical performance, we monitored ppGpp in Escherichia coli under different nutritional conditions and studied the relationship between ppGpp and DNA repair, which is helpful for overcoming antibiotic resistance and promoting the development of potential drugs for antibacterial treatment.

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“…Understanding disease states and developing potential drugs require precise recognition and measurement of key biomolecules in cells, such as ROS and phosphorylated proteins/peptides. Tremendous efforts have been made to the discover ROS sensors using fluorescence sensing, including a copper nanocluster–based nanoprobe for •OH and O 2 •– , MoO 3 NPs-G-CDs toward ClO – , a ratiometric fluorescence biosensor toward •OH, a glutathione (GSH)-modified fluorescent Au nanoclusters (GSH-AuNCs) toward •OH, ClO – , ONOO – , ROS-sensitive neural microelectrodes − are also essential in biological research. In terms of phosphoproteomics analysis, a mainstream strategy combines phosphopeptide enrichment and high-resolution mass spectrometry analysis to explore phosphorylation–related signaling pathways. , Although detection of ROS and phosphorylated proteins/peptides are often considered independent, the integration of both into one system could facilitate exploring the interplay between oxidation and phosphorylation events.…”

mentioning

confidence: 99%

H₂O₂ and Phosphorylated Peptide Dual-Responsive Nanochannel Device

Peng,

Wang,

Lin

et al. 2023

Anal. Chem.

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Oxidation and protein phosphorylation are critical mechanisms involved in regulating various cellular activities. Increasing research has suggested that oxidative stress could affect the activities of specific kinases or phosphatases, leading to alterations in the phosphorylation status of certain proteins. Ultimately, these alterations can affect cellular signaling pathways and gene expression patterns. However, the relationship between oxidation and protein phosphorylation remains complex and not yet fully understood. Therefore, the development of effective sensors capable of detecting both oxidation and protein phosphorylation simultaneously presents an ongoing challenge. To address this need, we introduce a proof-of-concept nanochannel device that is dual-responsive to both H2O2 and phosphorylated peptide (PP). Specifically, we design a peptide GGGCEG(GPGGA)4CEGRRRR, which contains an H2O2-sensitive unit CEG, an elastic peptide fragment (GPGGA)4, and a phosphorylation site recognition fragment RRRR. When the peptides are immobilized on the inner walls of conical nanochannels in a polyethylene terephthalate membrane, this peptide-modified nanochannel device exhibits a sensitive response to both H2O2 and PPs. The peptide chains undergo a random coil-to-α-helix transition in response to H2O2, which leads to a close-to-open transition of the nanochannel, accompanied with a remarkable increase in the transmembrane ionic current. In contrast, binding of the peptides with PPs shields the positive charge of the RRRR fragments, causing a decrease of the transmembrane ionic current. These unique features enable the sensitive detection of reactive oxygen species released by 3T3-L1 cells stimulated by platelet-derived growth factor (PDGF) as well as PDGF-induced change in the PP level. Real-time kinase activity monitoring further confirms the device’s potential utility for kinase inhibitor screening.

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Rational design of a self-assembled surfactant film in nanopipettes: combined fluorescence and electrochemical sensing

Abstract: Herein, a generalizable method based on the formation of self-assembled surfactant film was reported to build a nanopipette system, which was applicable for both ionic current and fluorescent analysis of...

Cited by 5 publications

References 29 publications

Solid‐State Glass Nanopipettes: Functionalization and Applications

Solid‐State Glass Nanopipettes: Functionalization and Applications

Rare Earth-Carbon Dots Nanocomposite-Modified Glass Nanopipettes: Electro-Optical Detection of Bacterial ppGpp

H₂O₂ and Phosphorylated Peptide Dual-Responsive Nanochannel Device

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Rational design of a self-assembled surfactant film in nanopipettes: combined fluorescence and electrochemical sensing

Abstract: Herein, a generalizable method based on the formation of self-assembled surfactant film was reported to build a nanopipette system, which was applicable for both ionic current and fluorescent analysis of...

Cited by 5 publications

References 29 publications

Solid‐State Glass Nanopipettes: Functionalization and Applications

Solid‐State Glass Nanopipettes: Functionalization and Applications

Rare Earth-Carbon Dots Nanocomposite-Modified Glass Nanopipettes: Electro-Optical Detection of Bacterial ppGpp

H2O2 and Phosphorylated Peptide Dual-Responsive Nanochannel Device

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Product

Resources

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H₂O₂ and Phosphorylated Peptide Dual-Responsive Nanochannel Device