Rate of Incorporation of R3hlthymidine In Various Tissues of the Mouse A basis for the evaluation of genotoxic effects of chemicals

Abstract

13HlThymidine has been extensively used as a selective pr6cursor to DNA in studies on the kinetics of cell proliferation. We have become interested in measuring early inhibition of the DNA synthesis in various organs of intact animals for detecting genotoxic properties of chemicals. Such experiments should, for convenience and to achieve a large capacity, be performed in the simplest way possible.The present paper deals with some practical aspects on the use of I3H]thymidine in uivo. C6-3HlThymidine was injected intraperitoneally in mice and the uptake of