Rat Resistance to Schistosomiasis: Platelet-Mediated Cytotoxicity Induced by C-Reactive Protein

Bout, Daniel; Joseph, M.; Pontet, Michel; Vorng, Han; Deslée, Didier; Càpron, André

doi:10.1126/science.3079916

Cited by 86 publications

(40 citation statements)

References 13 publications

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“…In addition, platelets have been shown many years ago to have Gram-positive bactericidal activity (39) and, more recently, to participate in the cytotoxicity against parasites responsible for schistosomiasis (40,41) and toxoplasmosis (42). There is also experimental evidence of cytotoxicity of platelets against tumor cells (43,44), with downmodulation of cell killing when platelets were pretreated with cyclooxygenase or lipoxygenase inhibitors (43) or protease inhibitors (44).…”

Section: Discussionmentioning

confidence: 99%

Platelets roll on stimulated endothelium in vivo: an interaction mediated by endothelial P-selectin.

Frenette

Johnson

Hynes

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

426

289

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P-selectin, found in storage granules ofplatelets and endothelial cells, can be rapidly expressed upon stimulation. Mice lacking this membrane receptor exhibit a severe impairment of leukocyte rolling. We observed that, in addition to leukocytes, platelets were rolling in mesenteric venules of wild-typej mice. To investigate the role of P-selectin in this process, resting or activated platelets from wild-type or P-selectin-deficient mice were fluorescently labeled and transfused into recipients of either genotype. Platelet-endothelial interactions were monitored by intravital microscopy. We observed rolling of either wild-type or P-selectin-deficient resting platelets on wild-type endothelium. Endothelial stimulation with the calcium ionophore A23187 increased the number of platelets rolling 4-fold. Activated P-selectindeficient platelets behaved similarly, whereas activated wildtype platelets bound to leukocytes and were seen rolling together. Platelets of either genotype, resting or activated, interacted minimally with mutant endothelium even after A23187 treatment. The velocity of platelet rolling was 6-to 9-fold greater than that of leukocytes. Our results demonstrate that (i) platelets roll on endothelium in vivo, (ii) this interaction requires endothelial but not platelet P-selectin, and (iii) platelet rolling appears to be independent of platelet activation, indicating constitutive expression of a P-selectin ligand(s) on platelets. We have therefore observed an interesting parallel between platelets and leukocytes in that both of these blood cell types roll on stimulated vessel wall and that this process is dependent on the expression of endothelial P-selectin.Rolling of blood cells along the endothelial surface of venules was first described >150 years ago when leukocytes were shown to adhere to the walls of blood vessels and this interaction increased in inflammation (1, 2). Subsequently, Atherton and Born (3) developed the modern version of intravital microscopy and made the first quantitative observations of leukocyte rolling in the cheek pouch of hamsters and in mouse mesentery. Leukocyte rolling is the initial stage of a multistep process leading to extravasation of white blood cells to sites of inflammation or infection (4, 5). The selectin family has been shown by in vitro experimentation (6) and by intravital microscopy (7-9) to mediate this rolling motion.One member of this family of adhesion molecules, Pselectin, is stored preformed in a granules of platelets (10, 11) and in Weibel-Palade bodies of endothelial cells (12, 13). After stimulated secretion and expression on the cell surface, both platelet and endothelial P-selectin mediate adhesion to leukocytes (14, 15). Beside its role in binding to leukocytes, the biological relevance of platelet P-selectin, a specific marker of platelet activation, is still unknown. In vitro, neutrophils have been shown to roll on P-selectin of immobilized activated platelets (16). Endothelial P-selectin is a key mediator of leukocyte rolling and subseq...

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Section: Discussionmentioning

confidence: 99%

Platelets roll on stimulated endothelium in vivo: an interaction mediated by endothelial P-selectin.

Frenette

Johnson

Hynes

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

426

289

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“…In rats, Schistosoma infection causes an increase in serum levels of CRP, a positive acute-phase protein associated with VLDL and LDL. CRP has been shown to activate platelets and render them cytotoxic to schistosomula in vitro (370). Besides platelets, activated monocytes can kill schistosomula.…”

Section: Lipoproteins and Parasitesmentioning

confidence: 99%

Thematic review series: The Pathogenesis of Atherosclerosis. Effects of infection and inflammation on lipid and lipoprotein metabolism mechanisms and consequences to the host

Khovidhunkit

Kim

Memon

et al. 2004

Journal of Lipid Research

1,289

1,200

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“…Many authors have estimated the effect of IgE, TNF, IFN-γ , IL-6 and C-reactive protein on the cytotoxicity of blood platelets in the course of schistosomosis [6,[28][29][30][31][32]. It has been known that the release of toxic substances by platelets depends on the presence of IgE and specific antigens found in a soluble form or on the parasite [34].…”

Section: Studies Of Other Authors Have Shown That In Gmentioning

confidence: 99%

IFN-gamma, IL-5, IL-6 and IgE in patients infected with Giardia intestinalis.

Matowicka-Karna

Dymicka-Piekarska²,

Kemona³

2009

Folia Histochem Cytobiol.

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Abstract:The immune system, its cellular and humoral response, is engaged by the host organism to fight against parasitic infections. The study group consisted of 90 patients (58 women and 32 men), aged 18-72 years, infected with G. intestinalis. The diagnosis was established based on laboratory investigations (stool examination, choloscopy, GSA-65). Blood for analysis was collected before (G1), and 2 weeks (G2) and 2 months (G3) after antiparasitic treatment. Control group consisted of 40 healthy subjects (22 women and 18 men), aged 20-45 years. The concentrations of IgE were assayed using a set of VIDAS (bioMerieux) and the concentrations of IL-5, IL-6, IFN-γ were determined using a set of Quantikine human (R&D Systems). It was revealed that in giardiosis the concentrations of IgE and IL-5 in blood serum were twice as high, the concentration of IL-6 was two and a half times higher and the concentration of IFN-γ was almost four times higher as compared to healthy controls.

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Rat Resistance to Schistosomiasis: Platelet-Mediated Cytotoxicity Induced by C-Reactive Protein

Cited by 86 publications

References 13 publications

Platelets roll on stimulated endothelium in vivo: an interaction mediated by endothelial P-selectin.

Platelets roll on stimulated endothelium in vivo: an interaction mediated by endothelial P-selectin.

Thematic review series: The Pathogenesis of Atherosclerosis. Effects of infection and inflammation on lipid and lipoprotein metabolism mechanisms and consequences to the host

IFN-gamma, IL-5, IL-6 and IgE in patients infected with Giardia intestinalis.

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