Rat palatal histology related to denture-like appliances

Candida-Associated Denture Stomatitis and Murine Models: What Is the Importance and Scientific Evidence?

“…Despite these favorable results with the adjustment of palatal intraoral devices, it is important to emphasize that CADS induction was not evaluated by Hotta et al [61,62]. On the other hand, there was no standardization of oral devices for such parameters in previous investigations of CADS induction animal models [27,39,[41][42][43][44][45][46][47][48][49].…”

“…The rodent animals of choice for a model murine of CADS induction are rats and not mice, since the former have a cavity compatible with the use of acrylic intraoral devices simulating dentures [27,39,[41][42][43][44][45]. Moreover, the clinical and histologic findings in the rats' palatal tissues of experimental CADS are similar to those of humans [39].…”

Candida-Associated Denture Stomatitis and Murine Models: What Is the Importance and Scientific Evidence?

“…Despite these favorable results with the adjustment of palatal intraoral devices, it is important to emphasize that CADS induction was not evaluated by Hotta et al [61,62]. On the other hand, there was no standardization of oral devices for such parameters in previous investigations of CADS induction animal models [27,39,[41][42][43][44][45][46][47][48][49].…”

“…The rodent animals of choice for a model murine of CADS induction are rats and not mice, since the former have a cavity compatible with the use of acrylic intraoral devices simulating dentures [27,39,[41][42][43][44][45]. Moreover, the clinical and histologic findings in the rats' palatal tissues of experimental CADS are similar to those of humans [39].…”

Experimental animal models for denture stomatitis: A methodological review

“…The first reported devices covered the entire hard palate and were constructed with cold-cured acrylic resin 21,29 or heat-cured acrylic resin. 30,31 To keep devices in position, researchers incorporated a metallic band around the maxillary incisors 29–31 (Figure 1) or cemented them with cold-cure acrylic resin around the maxillary incisor teeth (Figure 2). 21 Another fixation method later described is the incorporation of stainless steel wires in the incisive region.…”

“…Ill fitted devices can either be lost through the course of the experiment or make the palatal tissue more susceptible to fungal infestation, considering it would probably cause more local trauma. 31…”

“…Given that the intraoral device use is essential for the development of denture stomatitis in animals, diet adequacy is important to keep devices in position, avoid debris accumulation and ensure the animals’ well-being during experiments. Some studies used the conventional pellet diet 21,35,43,44 but this approach may result in significant amounts of debris accumulated between devices and the epithelium, 30,31 which can compromise the experiments.…”

Candida-Associated Denture Stomatitis and Murine Models: What Is the Importance and Scientific Evidence?

“…Despite these favorable results with the adjustment of palatal intraoral devices, it is important to emphasize that CADS induction was not evaluated by Hotta et al [61,62]. On the other hand, there was no standardization of oral devices for such parameters in previous investigations of CADS induction animal models [27,39,[41][42][43][44][45][46][47][48][49].…”

“…The rodent animals of choice for a model murine of CADS induction are rats and not mice, since the former have a cavity compatible with the use of acrylic intraoral devices simulating dentures [27,39,[41][42][43][44][45]. Moreover, the clinical and histologic findings in the rats' palatal tissues of experimental CADS are similar to those of humans [39].…”

Experimental animal models for denture stomatitis: A methodological review

“…The first reported devices covered the entire hard palate and were constructed with cold-cured acrylic resin 21,29 or heat-cured acrylic resin. 30,31 To keep devices in position, researchers incorporated a metallic band around the maxillary incisors 29–31 (Figure 1) or cemented them with cold-cure acrylic resin around the maxillary incisor teeth (Figure 2). 21 Another fixation method later described is the incorporation of stainless steel wires in the incisive region.…”

“…Ill fitted devices can either be lost through the course of the experiment or make the palatal tissue more susceptible to fungal infestation, considering it would probably cause more local trauma. 31…”

“…Given that the intraoral device use is essential for the development of denture stomatitis in animals, diet adequacy is important to keep devices in position, avoid debris accumulation and ensure the animals’ well-being during experiments. Some studies used the conventional pellet diet 21,35,43,44 but this approach may result in significant amounts of debris accumulated between devices and the epithelium, 30,31 which can compromise the experiments.…”

Candida-Associated Denture Stomatitis and Murine Models: What Is the Importance and Scientific Evidence?

“…Despite these favorable results with the adjustment of palatal intraoral devices, it is important to emphasize that CADS induction was not evaluated by Hotta et al [61,62]. On the other hand, there was no standardization of oral devices for such parameters in previous investigations of CADS induction animal models [27,39,[41][42][43][44][45][46][47][48][49].…”

“…The rodent animals of choice for a model murine of CADS induction are rats and not mice, since the former have a cavity compatible with the use of acrylic intraoral devices simulating dentures [27,39,[41][42][43][44][45]. Moreover, the clinical and histologic findings in the rats' palatal tissues of experimental CADS are similar to those of humans [39].…”

Experimental animal models for denture stomatitis: A methodological review

“…The first reported devices covered the entire hard palate and were constructed with cold-cured acrylic resin 21,29 or heat-cured acrylic resin. 30,31 To keep devices in position, researchers incorporated a metallic band around the maxillary incisors 29–31 (Figure 1) or cemented them with cold-cure acrylic resin around the maxillary incisor teeth (Figure 2). 21 Another fixation method later described is the incorporation of stainless steel wires in the incisive region.…”

“…Ill fitted devices can either be lost through the course of the experiment or make the palatal tissue more susceptible to fungal infestation, considering it would probably cause more local trauma. 31…”

“…Given that the intraoral device use is essential for the development of denture stomatitis in animals, diet adequacy is important to keep devices in position, avoid debris accumulation and ensure the animals’ well-being during experiments. Some studies used the conventional pellet diet 21,35,43,44 but this approach may result in significant amounts of debris accumulated between devices and the epithelium, 30,31 which can compromise the experiments.…”