Rat Intestinal Acetic Acid and Butyric acid and Effects of Age, Sex, and High-fat Diet on the Intestinal Levels in Rats

Choi, Soo In; Kim, Nayoung; Lee, Sun Min; Nam, Ryoung Hee; Kang, So Ra; Song, Chin Hee; Park, Young Tae; Min, Huitae; Kim, Yeon Ran; Seok, Yeong‐Jae

doi:10.15430/jcp.2019.24.1.20

Cited by 7 publications

(4 citation statements)

References 24 publications

(23 reference statements)

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“…The daily calorie intake and the gained body weight during the 8-week HFD feeding period were reported in our previous study [25]. The weight gain was decreased with age both in male and female rats.…”

Section: Compositional Changes Depend On Sex Age and Dietsupporting

confidence: 61%

Fecal Microbial Enterotypes Differentially Respond to a High-fat Diet Based on Sex in Fischer-344 Rats

et al. 2021

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The gut microbiota interacts with the host gut environment, which is influenced by such factors as sex, age, and host diet. These factors induce changes in the microbial composition. The aim of this study was to identify differences in the gut microbiome of Fisher-344 (F344) rats fed a high-fat diet (HFD), depending on their age and sex. Fecal microbiomes from 6-, 31-, and 74-week-old, and 2-year-old both male and female rats (corresponding to 5-, 30-, 60-, and 80-year-old humans) were analyzed using 16S rRNA gene sequencing, phylogenetic investigation of communities by reconstruction of unobserved states, and enterotype (E) assessment. Moreover, the effect of an HFD on colonic epithelial cells was measured using real-time quantitative PCR. Alpha diversity decreased in the HFD group regardless of age and sex. Based on the enterotype clustering of the whole fecal microbiome, clusters from male rats were divided into E1 and E2 enterotypes, while clusters from female rats were divided into E1, E2, and E3 enterotypes. The female E3 group showed a significantly high abundance in the Ruminococcus genus and expression of Tlr2 mRNA, which may reflect compensation to the HFD. Moreover, the female E3 group showed a lower ratio of opportunistic pathogenic strains to commensal strains compared to the female E2 group. Administration of an HFD influenced the rat fecal microbiota in all assessed age groups, which could be further differentiated by sex. In particular, female rats showed a compensatory enterotype response to an HFD compared to male rats.

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Section: Compositional Changes Depend On Sex Age and Dietsupporting

confidence: 61%

Fecal Microbial Enterotypes Differentially Respond to a High-fat Diet Based on Sex in Fischer-344 Rats

et al. 2021

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“…Butyric acid, a short‐chain fatty acid, induces cell morphology changes and cell differentiation in different cell types 22 . However, the direct effects of this short‐chain fatty acid on bone cells have not been well studied.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the levels of osteoblast stimulation were positively related to the metabolite concentration and the duration of incubation. In contrast, no significant increase in osteodifferentiation in different cell types 22. However, the direct effects of this short-chain fatty acid on bone cells have not been well studied.…”

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confidence: 89%

The role ofBacillus acidophilusin osteoporosis and its roles in proliferation and differentiation

Chen

Dong

Wang

et al. 2020

Clinical Laboratory Analysis

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“…Butyric acid, a short-chain fatty acid, induces cell morphology changes and cell differentiation in different cell types (22).However, the direct effects of this short-chain fatty acid on bone cells have not been well studied. In recent years, studies have shown that ALP and OCN are expressed throughout the processes of proliferation and differentiation in MC3T3-E1 cells.…”

Section: Discussionmentioning

confidence: 99%

The role of Bacillus acidophilus in osteoporosis and its roles in osteocyte proliferation and differentiation

Chen

Dong

Wang

et al. 2019

Preprint

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The microbes play an important role to physiological activities of human. Osteoporosis is one of the most closely relationship diseases with the elderly. In recent years, the studies found that gut microbiota can cause osteoporosis. These microbes may affect activity of osteoclasts and osteoblasts to affect bone metabolism. We selected 5 health people and 10 osteoporosis patients and found that the diversity of gut microbiota in osteoporosis patients are decreased and imbalance with lower abundance of lactobacillus and butylic acid bacteria, meanwhile, 25-hydroxyitamin D and procollagen type I N-terminal peptide (PINP) of osteoporosis patient was significantly lower than the normal group. The proliferation, differentiation and maturity of osteoblasts MC3T3-E1 cells was stimulated, the activity of alkaline phosphatase, concentration of osteocalcin and the expression of RUNX2, WNT2 and CTNNB1 was improved by supernatant of lactobacillus acidophilus and butanoic acids, however, the proliferation, differentiation and maturity, the expression of RANK,WNT2 and CTNNB1 in osteoclasts RAW 264.7 cells was suppressed. There is not a similar significant effect by lactobacillus rhamnosus treatment.

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Rat Intestinal Acetic Acid and Butyric acid and Effects of Age, Sex, and High-fat Diet on the Intestinal Levels in Rats

Cited by 7 publications

References 24 publications

Fecal Microbial Enterotypes Differentially Respond to a High-fat Diet Based on Sex in Fischer-344 Rats

Fecal Microbial Enterotypes Differentially Respond to a High-fat Diet Based on Sex in Fischer-344 Rats

The role ofBacillus acidophilusin osteoporosis and its roles in proliferation and differentiation

The role of Bacillus acidophilus in osteoporosis and its roles in osteocyte proliferation and differentiation

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