Rapidly achieving partial nitrification of municipal wastewater in enhanced biological phosphorus removal (EBPR) reactor: Effect of heterotrophs proliferation and microbial interactions

Sun, Tao; Du, Rui; Dan, Qiongpeng; Liu, Ying; Peng, Yongzhen

doi:10.1016/j.biortech.2021.125712

Cited by 32 publications

(6 citation statements)

References 41 publications

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“…Most previous studies that achieved the simultaneous EBPR and nitritation targeting the low-strength municipal wastewaters, low DO control (<1 mg/L), FA, and FNA inhibition were regarded as the commonly used effective strategies to suppress NOB. ,− In this study, DO at the aerobic phase was not controlled and could reach the saturation level (8 mg/L); besides, the FA (∼1 mg/L) and FNA ((0.2–0.7) × 10 –3 mg/L) were below the typical concentration range that was reported to cause the inhibition of NOB (1–10 mg/L FA and 0.2–2.8 mg/L FNA) . Overall, these commonly used NOB suppression strategies do not apply to our system.…”

Section: Discussionmentioning

confidence: 73%

Toward Integrating EBPR and the Short-Cut Nitrogen Removal Process in a One-Stage System for Treating High-Strength Wastewater

Kang,

Yuan,

et al. 2023

Environ. Sci. Technol.

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Enhanced biological phosphorus removal (EBPR) is an economical and sustainable process for phosphorus removal from wastewater. Despite the widespread application of EBPR for low-strength domestic wastewater treatment, limited investigations have been conducted to apply EBPR to the high-strength wastewaters, particularly, the integration of EBPR and the short-cut nitrogen removal process in the one-stage system remains challenging. Herein, we reported a novel proof-of-concept demonstration of integrating EBPR and nitritation (oxidation of ammonium to nitrite) in a one-stage sequencing batch reactor to achieve simultaneous high-strength phosphorus and short-cut nitrogen removal. Excellent EBPR performance of effluent 0.8 ± 1.0 mg P/L and >99% removal efficiency was achieved fed with synthetic high-strength phosphorus wastewater. Long-term sludge acclimation proved that the dominant polyphosphate accumulating organisms (PAOs), Candidatus Accumulibacter, could evolve to a specific subtype that can tolerate the nitrite inhibition as revealed by operational taxonomic unit (OTU)-based oligotyping analysis. The EBPR kinetic and stoichiometric evaluations combined with the amplicon sequencing proved that the Candidatus Competibacter, as the dominant glycogen accumulating organisms (GAOs), could well coexist with PAOs (15.3−24.9% and 14.2−33.1%, respectively) and did not deteriorate the EBPR performance. The nitrification activity assessment, amplicon sequencing, and functional-based gene marker quantification verified that the unexpected nitrite accumulation (10.7−21.0 mg N/L) in the high-strength EBPR system was likely caused by the nitritation process, in which the nitrite-oxidizing bacteria (NOB) were successfully out-selected (<0.1% relative abundance). We hypothesized that the introduction of the anaerobic phase with high VFA concentrations could be the potential selection force for achieving nitritation based on the literature review and our preliminary batch tests. This study sheds light on developing a new feasible technical route for integrating EBPR with short-cut nitrogen removal for efficient high-strength wastewater treatment.

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Section: Discussionmentioning

confidence: 73%

Toward Integrating EBPR and the Short-Cut Nitrogen Removal Process in a One-Stage System for Treating High-Strength Wastewater

Kang,

Yuan,

et al. 2023

Environ. Sci. Technol.

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“…Previous studies have shown that Proteobacteria are the main dominant phylum commonly found in activated sludge systems, containing a variety of micro-organisms that degrade organic pollutants and remove nutrients such as nitrogen (e.g., DNB, etc.) [61,62]. Therefore, the decrease in the TN removal capacity with the addition of PLA MPs may be related to the decrease in the relative abundance of Proteobacteria.…”

Section: Microbial Compositionmentioning

confidence: 99%

Impacts of Polylactic Acid Microplastics on Performance and Microbial Dynamics in Activated Sludge System

Huang,

Wang,

Zhang

et al. 2023

Sustainability

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A large number of microplastics (MPs) have been found in various stages of wastewater treatment plants, which may affect the functional microbial activity in activated sludge and lead to unstable pollutant removal performance. In this study, the effects of different concentrations of polylactic acid microplastics (PLA MPs) on system performance, nitrification and phosphorus (P) removal activities, and extracellular polymeric substances (EPS) were evaluated. The results showed that under the same influent conditions, low concentrations (50 particles/(g TS)) of PLA MPs had no significant effect on effluent quality. The average removal efficiencies of chemical oxygen demand, phosphate, and ammonia were all above 80%, and the average removal efficiencies of total nitrogen remained above 70%. High concentrations (200 particles/(g TS)) of PLA MPs inhibited the activities of polyphosphate-accumulating organisms (PAOs) and nitrifying bacteria. The specific anaerobic P release rate decreased from 37.7 to 23.1 mg P/(g VSS·h), and the specific aerobic P uptake rate also significantly decreased. The specific ammonia oxidation rate decreased from 0.67 to 0.34 mg N/(g VSS·h), while the change in the specific nitrite oxidation rate was not significant. The dosing of PLA MPs decreased the total EPS and humic acid content. As the concentration of PLA MPs increased, microbial community diversity increased. The relative abundance of potential PAOs (i.e., Acinetobacter) increased from 0.08 to 12.57%, while the relative abundance of glycogen-accumulating organisms (i.e., Competibacter and Defluviicoccus) showed no significant changes, which would lead to improved P removal performance. The relative abundance of denitrifying bacteria (i.e., Pseudomonas) decreased from 95.43 to 58.98%, potentially contributing to the decline in denitrification performance.

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“…Co-inoculation of more than one AOB at high concentrations ensures a shorter adaptation time of the bacteria to WW, ammonium oxidation from the beginning of the process and survival of the same AOB for extensive times (Zhou et al 2020 ; Moloantoa et al 2022 ). Also, AOBs develop cooperative interactions with WW heterotrophic and photosynthetic microorganisms to receive mutual benefit and improve NH 4 + removal efficiency (Zhou et al 2020 ; Sun et al 2021 ). In this interaction, the heterotrophic microorganisms produce the CO 2 necessary for the AOBs to obtain their inorganic carbon source, and after oxidising NH 4 + , they decrease the toxic effect of this compound for the heterotrophic microorganisms (Zhou et al 2020 ; Fan et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…In this interaction, the heterotrophic microorganisms produce the CO 2 necessary for the AOBs to obtain their inorganic carbon source, and after oxidising NH 4 + , they decrease the toxic effect of this compound for the heterotrophic microorganisms (Zhou et al 2020 ; Fan et al 2021 ). Finally, WW microorganisms may also include bacteria that oxidise NO 2 − to NO 3 − ; the NO 3 − is reduced by the microorganisms from the WW by assimilative pathway or can be recovered through different physical or chemical processes as a strategy for recycling nutrients from wastewater (Cruz et al 2018 ; Spasov et al 2020 ; Li et al 2021 ; Sun et al 2021 ; Moloantoa et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Wastewater treatment from a science faculty during the COVID-19 pandemic by using ammonium-oxidising and heterotrophic bacteria

Pedroza-Camacho,

Ospina-Sánchez,

Romero-Perdomo

et al. 2024

3 Biotech

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During and after the pandemic caused by the SARS-CoV-2 virus, the use of personal care products and disinfectants increased in universities worldwide. Among these, quaternary ammonium-based products stand out; these compounds and their intermediates caused substantial changes in the chemical composition of the wastewater produced by these institutions. For this reason, improvements and environmentally sustainable biological alternatives were introduced in the existing treatment systems so that these institutions could continue their research and teaching activities. For this reason, the objective of this study was to develop an improved culture medium to cultivate ammonium oxidising bacteria (AOB) to increase the biomass and use them in the treatment of wastewater produced in a faculty of sciences in Bogotá, D.C., Colombia. A Plackett Burman Experimental Design (PBED) and growth curves served for oligotrophic culture medium, and production conditions improved for the AOB. Finally, these bacteria were used with total heterotrophic bacteria (THB) for wastewater treatment in a pilot plant. Modification of base ammonium broth and culture conditions (6607 mg L−1 of (NH4)2SO4, 84 mg L−1 CaCO3, 40 mg L−1 MgSO4·7H2O, 40 mg L−1 CaCl2·2H2O and 200 mg L−1 KH2PO4, 10% (w/v) inoculum, no copper addition, pH 7.0 ± 0.2, 200 r.p.m., 30 days) favoured the growth of Nitrosomonas europea, Nitrosococcus oceani, and Nitrosospira multiformis with values of 8.23 ± 1.9, 7.56 ± 0.7 and 4.2 ± 0.4 Log10 CFU mL−1, respectively. NO2− production was 0.396 ± 0.0264, 0.247 ± 0.013 and 0.185 ± 0.003 mg L−1 for Nitrosomonas europea, Nitrosococcus oceani and Nitrosospira multiformis. After the 5-day wastewater treatment (WW) by co-inoculating the three studied bacteria in the wastewater (with their self-microorganisms), the concentrations of AOB and THB were 5.92 and 9.3 Log10 CFU mL−1, respectively. These values were related to the oxidative decrease of Chemical Oxygen Demand (COD), (39.5 mg L−1), Ammonium ion (NH4+), (6.5 mg L−1) Nitrite (NO2−), (2.0 mg L−1) and Nitrate (NO3−), (1.5 mg L−1), respectively in the five days of treatment. It was concluded, with the improvement of a culture medium and production conditions for three AOB through biotechnological strategies at the laboratory scale, being a promising alternative to bio-augment of the biomass of the studied bacteria under controlled conditions that allow the aerobic removal of COD and nitrogen cycle intermediates present in the studied wastewater.

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Rapidly achieving partial nitrification of municipal wastewater in enhanced biological phosphorus removal (EBPR) reactor: Effect of heterotrophs proliferation and microbial interactions

Cited by 32 publications

References 41 publications

Toward Integrating EBPR and the Short-Cut Nitrogen Removal Process in a One-Stage System for Treating High-Strength Wastewater

Toward Integrating EBPR and the Short-Cut Nitrogen Removal Process in a One-Stage System for Treating High-Strength Wastewater

Impacts of Polylactic Acid Microplastics on Performance and Microbial Dynamics in Activated Sludge System

Wastewater treatment from a science faculty during the COVID-19 pandemic by using ammonium-oxidising and heterotrophic bacteria

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